NFYC

Nuclear transcription factor Y, gamma
PDB rendering based on 1n1j.
Available structures PDB 1n1j
Identifiers
Symbols	NFYC; CBF-C; CBFC; DKFZp667G242; FLJ45775; H1TF2A; HAP5; HSM; NF-YC
External IDs	OMIM: 605344 MGI: 107901 HomoloGene: 7440 GeneCards: NFYC Gene
Gene Ontology Molecular function • DNA binding • sequence-specific DNA binding transcription factor activity • transcription coactivator activity • protein binding • sequence-specific DNA binding Cellular component • intracellular • nucleus • CCAAT-binding factor complex Biological process • transcription, DNA-dependent • regulation of transcription, DNA-dependent • regulation of transcription from RNA polymerase II promoter • protein folding Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4802	18046
Ensembl	ENSG00000066136	ENSMUSG00000032897
UniProt	Q13952	Q7TPS7
RefSeq (mRNA)	NM_001142587.1	NM_001048168
RefSeq (protein)	NP_001136059.1	NP_001041633
Location (UCSC)	Chr 1: 41.16 – 41.24 Mb	Chr 4: 120.43 – 120.5 Mb
PubMed search	[1]	[2]

Nuclear transcription factor Y subunit gamma is a protein that in humans is encoded by the NFYC gene.^[1][2][3]

Function

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit (NFYB), a prerequisite for subunit A (NFYA) association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.^[3]

Two microRNAs; miR-30c and miR-30e are located within introns of the nfyc gene. These microRNAs are actively transcribed in human insulin-producing beta cells in the pancreatic islets that also show high expression of nfyc and CDH1 genes. The expression of these intronic microRNAs is essential for maintaining the differentiated phenotype of human islet beta cells. Inhibition of miR-30 family microRNAs induces epithelial-mesenchymal transition of human pancreatic islet cells.^[4]

Interactions

NFYC has been shown to interact with Myc.^[5]

References

1. ^ Sinha S, Maity SN, Seldin MF, de Crombrugghe B (Feb 1997). "Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor". Genomics 37 (2): 260–3. doi:10.1006/geno.1996.0555. PMID 8921405. 
2. ^ Bellorini M, Zemzoumi K, Farina A, Berthelsen J, Piaggio G, Mantovani R (Aug 1997). "Cloning and expression of human NF-YC". Gene 193 (1): 119–25. doi:10.1016/S0378-1119(97)00109-1. PMID 9249075. 
3. ^ ^{a b} "Entrez Gene: NFYC nuclear transcription factor Y, gamma". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4802. 
4. ^ Joglekar MV, Patil D, Joglekar VM, Rao GV, Reddy DN, Mitnala S, Shouche Y, Hardikar AA (September/October 2009). "The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells". Islets 1 (2): 137–147. doi:10.4161/isl.1.2.9578. PMID 21099261. 
5. ^ Taira T, Sawai M, Ikeda M, Tamai K, Iguchi-Ariga SM, Ariga H (August 1999). "Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y". J. Biol. Chem. 274 (34): 24270–9. doi:10.1074/jbc.274.34.24270. PMID 10446203. 

Further reading

• Mantovani R (1999). "The molecular biology of the CCAAT-binding factor NF-Y.". Gene 239 (1): 15–27. doi:10.1016/S0378-1119(99)00368-6. PMID 10571030. 
• Martinelli R, Heintz N (1994). "H1TF2A, the large subunit of a heterodimeric, glutamine-rich CCAAT-binding transcription factor involved in histone H1 cell cycle regulation.". Mol. Cell. Biol. 14 (12): 8322–32. PMC 359371. PMID 7969168. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=359371. 
• Nakshatri H, Bhat-Nakshatri P, Currie RA (1997). "Subunit association and DNA binding activity of the heterotrimeric transcription factor NF-Y is regulated by cellular redox.". J. Biol. Chem. 271 (46): 28784–91. doi:10.1074/jbc.271.46.28784. PMID 8910521. 
• Dmitrenko V, Garifulin O, Kavsan V (1997). "Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor.". Gene 197 (1-2): 161–3. doi:10.1016/S0378-1119(97)00255-2. PMID 9332362. 
• Currie RA (1998). "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y).". J. Biol. Chem. 272 (49): 30880–8. doi:10.1074/jbc.272.49.30880. PMID 9388234. 
• Taira T, Sawai M, Ikeda M, et al. (1999). "Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y.". J. Biol. Chem. 274 (34): 24270–9. doi:10.1074/jbc.274.34.24270. PMID 10446203. 
• Yoshida H, Okada T, Haze K, et al. (2001). "Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response.". Mol. Cell. Biol. 21 (4): 1239–48. doi:10.1128/MCB.21.4.1239-1248.2001. PMC 99577. PMID 11158310. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99577. 
• Izumi H, Molander C, Penn LZ, et al. (2001). "Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor.". J. Cell. Sci. 114 (Pt 8): 1533–44. PMID 11282029. 
• Chen F, Ogawa K, Liu X, et al. (2002). "Repression of Smad2 and Smad3 transactivating activity by association with a novel splice variant of CCAAT-binding factor C subunit.". Biochem. J. 364 (Pt 2): 571–7. doi:10.1042/BJ20011703. PMC 1222603. PMID 12023901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1222603. 
• Romier C, Cocchiarella F, Mantovani R, Moras D (2003). "The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y.". J. Biol. Chem. 278 (2): 1336–45. doi:10.1074/jbc.M209635200. PMID 12401788. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Salsi V, Caretti G, Wasner M, et al. (2003). "Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function.". J. Biol. Chem. 278 (9): 6642–50. doi:10.1074/jbc.M210065200. PMID 12482752. 
• Hirose T, Sowa Y, Takahashi S, et al. (2003). "p53-independent induction of Gadd45 by histone deacetylase inhibitor: coordinate regulation by transcription factors Oct-1 and NF-Y.". Oncogene 22 (49): 7762–73. doi:10.1038/sj.onc.1207091. PMID 14586402. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Chattopadhyay C, Hawke D, Kobayashi R, Maity SN (2004). "Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro.". Nucleic Acids Res. 32 (12): 3632–41. doi:10.1093/nar/gkh692. PMC 484179. PMID 15243141. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=484179. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Wang B, Gao C, Ponder KP (2005). "C/EBPbeta contributes to hepatocyte growth factor-induced replication of rodent hepatocytes.". J. Hepatol. 43 (2): 294–302. doi:10.1016/j.jhep.2005.02.029. PMID 15922473. 

External links

• MeSH NFYC+protein,+human

This article incorporates text from the United States National Library of Medicine, which is in the public domain.