Methylene blue

Methylene blue
Methylene blue
Identifiers
CAS number 61-73-4 YesY
PubChem 6099
ChemSpider 5874 YesY
UNII 8NAP7826UB YesY
ChEBI CHEBI:6872 YesY
ChEMBL CHEMBL405110 YesY
Jmol-3D images Image 1
Properties
Molecular formula C16H18N3SCl
Molar mass 319.85 g/mol
Melting point

100-110 °C (with decomposition)

Boiling point

Decomposes

 N (verify) (what is: YesY/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Methylene blue (CI 52015) is a heterocyclic aromatic chemical compound with the molecular formula C16H18N3SCl. It has many uses in a range of different fields, such as biology and chemistry. At room temperature it appears as a solid, odorless, dark green powder, that yields a blue solution when dissolved in water. The hydrated form has 3 molecules of water per molecule of methylene blue.[1] Methylene blue should not be confused with methyl blue, another histology stain, new methylene blue, nor with the methyl violets often used as pH indicators.

The International Nonproprietary Name (INN) of methylene blue is methylthioninium chloride.[2][3]

Contents

Uses

Chemistry

A volumetric flask of a methylene blue solution

Redox indicator

Methylene blue is widely used as a redox indicator in analytical chemistry. Solutions of this substance are blue when in an oxidizing environment, but will turn colorless if exposed to a reducing agent. The redox properties can be seen in a classical demonstration of chemical kinetics in general chemistry, the "blue bottle" experiment. Typically, a solution is made of glucose (dextrose), methylene blue, and sodium hydroxide. Upon shaking the bottle, oxygen oxidizes methylene blue, and the solution turns blue. The dextrose will gradually reduce the methylene blue to its colorless, reduced form. Hence, when the dissolved dextrose is entirely consumed, the solution will turn blue again.

Peroxide generator

Methylene blue is also a photosensitizer used to create singlet oxygen when exposed to both oxygen and light. It is used in this regard to make organic peroxides by a Diels-Alder reaction which is spin forbidden with normal atmospheric triplet oxygen.

Sulfide analysis

The formation of methylene blue after the reaction of hydrogen sulfide with dimethyl-p-phenylenediamine and iron(III) at pH 0.4 – 0.7 is used to determine by photometric measurements sulfide concentration in the range 0.020 to 1.50 mg/L (20 ppb to 1.5 ppm). The test is very sensitive and the blue coloration developing upon contact of the reagents with dissolved H2S is stable for 60 min. Ready-to-use kits such as the Spectroquant sulfide test[4] facilitate routine analyses. The methylene blue sulfide test is a convenient method often used in soil microbiology to quickly detect in water the metabolic activity of sulfate reducing bacteria (SRB). It should be observed that in this test, methylene blue is a product of reaction and not a reagent.

The addition of a strong reducing agent, such as ascorbic acid, to a sulfide-containing solution is sometimes used to prevent sulfide oxidation from atmospheric oxygen. Although it is certainly a sound precaution for the determination of sulfide with an ion selective electrode, it might however hamper the development of the blue color if the freshly formed methylene blue is also reduced, as described here above in the paragraph on redox indicator.

Water testing

A color reaction in an acidified, aqueous methylene blue solution containing chloroform can detect anionic surfactants in a water sample. Such a test is known as an MBAS assay (methylene blue active substances assay).

The MBAS assay cannot distinguish between specific surfactants, however. Some examples of anionic surfactants are carboxylates, phosphates, sulfates, and sulfonates.

Biology

In biology methylene blue is used as a dye for a number of different staining procedures, such as Wright's stain and Jenner's stain. Since it is a temporary staining technique, methylene blue can also be used to examine RNA or DNA under the microscope or in a gel: as an example, a solution of methylene blue can be used to stain RNA on hybridization membranes in northern blotting to verify the amount of nucleic acid present. While methylene blue is not as sensitive as ethidium bromide, it is less toxic and it does not intercalate in nucleic acid chains, thus avoiding interference with nucleic acid retention on hybridization membranes or with the hybridization process itself.

It can also be used as an indicator to determine if a cell such as yeast is alive or not. The blue indicator turns colorless indicating living cells. However, if it stays blue it doesn't mean that the cell is dead or there are no cells. Methylene blue can inhibit the respiration of the yeast as it picks up hydrogen ions made during the process and the yeast cell cannot then use those ions to release energy.

In neuroscience, methylene blue can also serve as a non-selective inhibitor of NO synthase.

Medicine

Methylene blue is a monoamine oxidase inhibitor (MAOI),[5] and if infused intravenously at doses exceeding 5 mg/kg, may precipitate serious serotonin toxicity, serotonin syndrome, if combined with any selective serotonin reuptake inhibitors (SSRIs) or other serotonin reuptake inhibitor (e.g., duloxetine, sibutramine, venlafaxine, clomipramine, imipramine).[6]

Methylene blue is also structurally similar to the chlorpromazine and the typical antipsychotics. It is the basic compound from which chlorpromazine and many other antipsychotics are made.[7]

Methylene blue is a component of a frequently prescribed urinary analgesic/anti-infective/anti-spasmodic known as "Prosed", which also contains methanamine, hyoscyamine sulfate, and salicylate. A similar, more popular combination medication known as "Urised" (and its generic counterparts) was discontinued in 2007, quite possibly because of the way in which methanamine was included into the formulation (and its ease of separation from the other components of the medication), and the fact that methanamine is a precursor to methamphetamine, making it a target for clandestine use. The new formulation ("Prosed") sequesters the methanamine using a method which makes its separation nearly impossible.

Malaria

Methylene blue was identified by Paul Ehrlich about 1891 as a successful treatment for malaria. It disappeared as an anti-malarial during the Pacific War in the tropics, since American and Allied soldiers disliked its two prominent, but reversible side effects: turning the urine green, and the sclera (the whites of the eyes) blue. Interest in its use as an anti-malarial has recently been revived,[8] especially due to its low price. Several clinical trials are in progress, trying to find a suitable drug combination. Initial attempts to combine methylene blue with chloroquine were disappointing;[9] however, more recent attempts have appeared more promising.

Cancer

Recent research suggests that methylene blue, toluidine blue, and other 3,7-diaminophenothiazinium-based redox cyclers induce selective cancer cell apoptosis by NAD(P)H:quinone oxidoreductase (NQO1)-dependent bioreductive generation of cellular oxidative stress.[10] Combined with plant auxin (indole-3-acetic acid), methylene blue is being investigated for the photodynamic treatment of cancer.[11]

Combined with light

Methylene blue combined with light has been used to treat resistant plaque psoriasis,[12] AIDS-related Kaposi's sarcoma,[13] West Nile virus,[14] and to inactivate staphylococcus aureus,[15] HIV-1,[16] Duck hepatitis B,[17] adenovirus vectors,[18] and hepatitis C.[19] Phenothiazine dyes and light have been known to have virucidal properties for over 80 years.[20] In some circumstances, the combination can cause DNA damage that may lead to cancer.[21][22]

Methemoglobinemia

While many medical texts erroneously indicate that methylene blue has reducing agent properties, it has oxidizing agent properties. It is owing to this reason that methylene blue is employed as a medication for the treatment of methemoglobinemia. This can arise from ingestion of certain pharmaceuticals, toxins, or broad beans.[citation needed] Through the methemoglobin reductase enzyme (which is NADPH dependent), it is reduced by NADPH allowing it to have an affinity for methylene blue (among other dyes). As a result, methylene blue is reduced to leucomethylene blue, which then acts to reduce the heme group from methemoglobin to hemoglobin.[23] At high doses, however, methylene blue actually induces methemoglobinemia, reversing this pathway.[23]

Methylene blue also blocks accumulation of cyclic guanosine monophosphate (cGMP) by inhibiting the enzyme guanylate cyclase: this action results in reduced responsiveness of vessels to cGMP-dependent vasodilators like nitric oxide and carbon monoxide. Cardiac surgical teams have found this very useful in the treatment of extremely low blood pressure (hypotension)which may occur during heart surgery requiring cardiac bypass.[24] Similar use is noted in the treatment of hypotension associated with overwhelming infections (sepsis).[25]

Cyanide poisoning

Since its reduction potential is similar to that of oxygen and can be reduced by components of the electron transport chain, large doses of methylene blue are sometimes used as an antidote to potassium cyanide poisoning, a method first successfully tested in 1933 by Dr. Matilda Moldenhauer Brooks in San Francisco.[26]

Carbon monoxide poisoning

Methylene blue was also used in the mid-twentieth century in the treatment of carbon monoxide poisoning.[26]

Dye

Methylene blue is used in endoscopic polypectomy as an adjunct to saline or epinephrine, and is used for injection into the submucosa around the polyp to be removed. This allows the submucosal tissue plane to be identified after the polyp is removed, which is useful in determining if more tissue needs to be removed, or if there has been a high risk for perforation. Methylene blue is also used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions. Intravenously injected methylene blue is readily released into the urine and thus can be used to test the urinary tract for leaks or fistulas.

In surgeries such as sentinel lymph node dissections, methylene blue can be used to visually trace the lymphatic drainage of pertinent tissues. Similarly, methylene blue is added to bone cement in orthopedic operations to provide easy discrimination between native bone and cement. Additionally, methylene blue accelerates the hardening of bone cement, increasing the speed at which bone cement can be effectively applied.

It can also be used to stain lymph nodes.

Placebo

Methylene blue has been used as a placebo; physicians would tell their patients to expect their urine to change color and view this as a sign that their condition had improved.[27] This same side effect makes methylene blue difficult to test in traditional placebo-controlled clinical studies.[28]

Ifosfamide neurotoxicity

Another, less well-known use of methylene blue is its utility for treating ifosfamide neurotoxicity. Methylene blue was first reported for treatment and prophylaxis of ifosfamide neuropsychiatric toxicity in 1994. A toxic metabolite of ifosfamide, chloracetaldehyde (CAA), disrupts the mitochondrial respiratory chain, leading to an accumulation of nicotinamide adenine dinucleotide hydrogen (NADH). Methylene blue acts as an alternative electron acceptor, and reverses the NADH inhibition of hepatic gluconeogenesis while also inhibiting the transformation of chloroethylamine into chloroacetaldehyde, and inhibits multiple amine oxidase activities, preventing the formation of CAA.[29] The dosing of methylene blue for treatment of ifosfamide neurotoxicity varies, depending upon its use simultaneously as an adjuvant in ifosfamide infusion, versus its use to reverse psychiatric symptoms that manifest after completion of an ifosfamide infusion. Reports suggest that methylene blue at 50–60 mg up to six doses a day have resulted in improvement of symptoms within 10 minutes to several days.[30] Alternatively, it has been suggested that intravenous methylene blue 50 mg every six hours for prophylaxis during ifosfamide treatment in patients with history of ifosfamide neuropsychiatric toxicity.[31] Prophylactic administration of 50 mg of methylene blue the day before initiation of ifosfamide, and 50 mg three times daily during ifosfamide chemotherapy has been recommended to lower the occurrence of ifosfamide neurotoxicity.[32]

Clinical trials

TauRx Therapeutics has reported that methylene blue (methylthioninium chloride), under the tradename Rember, may provide a way of halting or slowing the progression of Alzheimer's dementia.[33] However, the formulation used was different from that commonly available as a medicine and caution has been expressed about use of methylene blue as a treatment for Alzheimer's.[34] TauRx Therapeutics has suggested that the mechanism by which methylene blue might delay or reverse neurodegeneration in Alzheimer's disease is as an inhibitor of Tau protein aggregation. While methylene blue arguably has an effect on Tau aggregation, it also has an effect on mitochondrial function which is likely to play an important role. In vitro studies suggest that methylene blue might be an effective remedy for both Alzheimer's and Parkinson's disease by enhancing key mitochondrial biochemical pathways. It can disinhibit and increase complex IV, whose inhibition correlates with Alzheimer's disease.

Methylene blue might also delay senescence as one study has shown that it extended the lifespan of IMR90 fibroblasts by more than 20 population doublings.[35]

These findings are highly controversial,[citation needed] and a clear dosage response curve has not been found.

Aquaculture

Methylene blue is used in aquaculture and by tropical fish hobbyists as a treatment for fungal infections. It can also be effective in treating fish infected with ich, the parasitic protozoa Ichthyophthirius multifiliis. It is usually used to protect newly laid fish eggs from being infected by fungus or bacteria. This is useful when the hobbyist wants to artificially hatch the fish eggs. Methylene Blue is also very effective when used as part of a "medicated fish bath" for treatment of ammonia, nitrite, and cyanide poisoning as well as for topical and internal treatment of injured or sick fish as a "first response".[36]

In popular culture

As a prank

It is, or was at one time, a common prank among college students in biomedical fields to spike someone's drink with methylene blue, thus creating amusement at the victim's expense when he reacts with alarm to his urine turning blue. With concern over date rape drugs, spiking someone's drink is considered far more serious than it used to be, and the prank has somewhat gone out of fashion.[37]

As a plot device

An episode of M*A*S*H, "Sons and Bowlers", showed Major Winchester using a dose of methylene blue to take down a rival camp's bowling champion—who had been a high-ranked professional bowler in civilian life—during a contest. The champ panics when his urine turns blue, and listens to Winchester's advice to refrain from all exercise – including bowling, which allows the 4077th to win.

In the 1946 film noir, Decoy, the chemical is portrayed in an entirely different manner - as having resuscitation qualities – in that it is used successfully to bring a criminal back to life after execution by hydrocyanic gas.[38][39]

Adverse reactions

Cardiovascular Central Nervous System Dermatologic Gastrointestinal Genito-urinary Hematologic
Hypertension
• Precordial pain
Dizziness
• Mental confusion
Headache
Fever
• Staining of skin
• Injection site necrosis (SC)
• Fecal discoloration
Nausea
Vomiting
• Abdominal pain
• Discoloration of urine
• Bladder irritation
Anemia

[40][41]

Causes hemolytic anemia in carriers of the G6PD (favism) enzymatic deficiency.

See also

References

  1. ^ http://www.methylene-blue.com/substance.php
  2. ^ Adams V., Marley J., McCarroll C. (November 2007). "Prilocaine induced methaemoglobinaemia in a medically compromised patient. Was this an inevitable consequence of the dose administered?". Br. Dent. J. 203 (10): 585–7. doi:10.1038/bdj.2007.1045. PMID 18037845. 
  3. ^ Linz A.J., Greenham R.K., Fallon L.F. (May 2006). "Methemoglobinemia: an industrial outbreak among rubber molding workers". J. Occup. Environ. Med. 48 (5): 523–8. doi:10.1097/01.jom.0000201815.32098.99. PMID 16688009. http://meta.wkhealth.com/pt/pt-core/template-journal/lwwgateway/media/landingpage.htm?an=00043764-200605000-00010. 
  4. ^ Spectroquant 114779 Sulfide Test. Method: photometric 0.020 - 1.50 mg/l S2-
  5. ^ Gillman P.K.; Ng, Bradley K.W.; Cameron, Andrew J.D.; Liang, Rhea W.Y. (May 2008). "Methylene blue is a potent monoamine oxidase inhibitor". Can. J. Anaesth. 55 (5): 311–312. doi:10.1007/BF03017212. PMID 18451123. 
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  7. ^ Healy, David (2002). The creation of psychopharmacology. Harvard University Press. ISBN 0674006194. 
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  21. ^ Sturmey R.G., Wild C.P., Hardie L.J. (May 2009). "Removal of red light minimizes methylene blue-stimulated DNA damage in oesophageal cells: implications for chromoendoscopy". Mutagenesis 24 (3): 253–8. doi:10.1093/mutage/gep004. PMID 19218330. 
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  24. ^ http://jtcs.ctsnetjournals.org/cgi/content/abstract/125/6/1426
  25. ^ http://www.medscape.com/viewarticle/409626
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  27. ^ Novella Steve. "The ethics of deception in medicine". Science Based Medicine. http://www.sciencebasedmedicine.org/?p=29. Retrieved 2008-01-24. 
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  29. ^ Alici-Evcimen Y., Breitbart W.S. (October 2007). "Ifosfamide neuropsychiatric toxicity in patients with cancer". Psychooncology 16 (10): 956–960. doi:10.1002/pon.1161. PMID 17278152. 
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  31. ^ Dufour C., Grill J., Sabouraud P. et al. (February 2006). "Ifosfamide induced encephalopathy: 15 observations" (in French). Arch. Pediatr. 13 (2): 140–145. doi:10.1016/j.arcped.2005.10.021. PMID 16364615. 
  32. ^ Aeschlimann T.; Cerny, T; Küpfer, A (1996). "Inhibition of (mono)amine oxidase activity and prevention of ifosfamide encephalopathy by methylene blue". Drug. Metab. Dispos. 24 (12): 1336–1339. PMID 8971139. 
  33. ^ "Alzheimer's drug 'halts' decline". BBC News. 2008-07-30. http://news.bbc.co.uk/1/hi/health/7525115.stm. Retrieved 2008-07-30. 
  34. ^ "Slowing disease's mental ravages". Chicago Tribune. 2008-07-30. http://www.chicagotribune.com/features/lifestyle/health/chi-alzheimers-wedjul30,0,3112870.story. [dead link]
  35. ^ Atamna H., Nguyen A., Schultz C., Boyle K., Newberry J., Kato H., Ames B.N. (March 2008). "Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways". FASEB J. 22 (3): 703–712. doi:10.1096/fj.07-9610com. PMID 17928358. 
  36. ^ [1] Aquarium Chemical Treatments
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  38. ^ Internet Movie Database entry for Decoy
  39. ^ Film Noir of the Week entry for Decoy
  40. ^ Mokhlesi B., Leikin J.B., Murray P., Corbridge T.C. (March 2003). "Adult toxicology in critical care: Part II: specific poisonings". Chest 123 (3): 897–922. doi:10.1378/chest.123.3.897. PMID 12628894. 
  41. ^ Harvey J.W., Keitt A.S. (May 1983). "Studies of the efficacy and potential hazards of methylene blue therapy in aniline-induced methaemoglobinaemia". Br. J. Haematol. 54 (1): 29–41. doi:10.1111/j.1365-2141.1983.tb02064.x. PMID 6849836. 

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Look at other dictionaries:

  • Methylene blue — Methylene Meth yl*ene, n. [F. m[ e]thyl[ e]ne, from Gr. ? wine + ? wood; a word coined to correspond to the name wood spirit.] (Chem.) A divalent hydrocarbon radical, { CH2 }, not known in the free state, but regarded as an essential residue and… …   The Collaborative International Dictionary of English

  • methylene blue — n. a bluish green aniline dye, C16H18N3ClS·3H2O, used as a bacteriologic stain, an antidote in cyanide poisoning, etc …   English World dictionary

  • methylene blue — noun a dark green dye used as a stain, an antiseptic, a chemical indicator, and an antidote in cyanide poisoning • Syn: ↑methylthionine chloride • Hypernyms: ↑stain, ↑thiazine * * * noun Usage: sometimes capitalized M&B : a basic dye of the… …   Useful english dictionary

  • methylene blue — A basic dye easily oxidized to azure, with dye mixtures; used in histology and microbiology, to stain intestinal protozoa in wet mount preparations, to track RNA and RNase in electrophoresis, and as an antidote for methemoglobinemia; its …   Medical dictionary

  • methylene blue — Chem., Pharm. a dark green, crystalline compound, C16H18ClN3S, that dissolves in water to form a deep blue solution: used chiefly as a dye, as a bacteriological and biological stain, and as an antidote for cyanide poisoning. Also called… …   Universalium

  • Methylene Blue — (= Swiss blue; Basic Blue 9; tetramethythionine chloride) Water soluble dye that can be reduced to a colourless form and can be oxidized by atmospheric oxygen. Used as a stain in bacteriology and histology …   Dictionary of molecular biology

  • methylene blue — meth′ylene blue′ n. pha chem. a dark green, crystalline compound, C16H18ClN3that dissolves in water to form a deep blue solution: used chiefly as a dye, as a biological stain, and as an antidote for cyanide poisoning …   From formal English to slang

  • methylene blue — a blue dye used to stain bacterial cells for microscopic examination …   The new mediacal dictionary

  • methylene blue — noun Date: circa 1890 a basic thiazine dye C16H18ClN3S•3H2O used especially as a biological stain, an antidote in cyanide poisoning, and an oxidation reduction indicator …   New Collegiate Dictionary

  • methylene blue — a dye (3,7 bis (Dimethylamino) phenazathionioum chloride) used against external bacteria and protozoans on fish …   Dictionary of ichthyology

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