Sudan stain

Sudan stain

Sudan staining is the use of Sudan dyes to stain sudanophilic substances, usually lipids. Sudan lysochromes (Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B) are used.

Sudan dyes have high affinity to fats, therefore they are used to demonstrate triglycerides, lipids, and lipoproteins.

Alcoholic solutions of Sudan dyes are usually used, however pyridine solutions can be used in some situations as well.

Sudan stain test is often used to determine the level of fecal fat to diagnose steatorrhea. Small sample is dissolved in water or saline, glacial acetic acid is added to hydrolyze the insoluble salts of fatty acids, few drops of alcoholic solution of Sudan III are added, the sample is spread on a microscopic slide, and heated twice to boil. Normally, a stool sample should show only a few drops of red-orange stained fat under the microscope. The method is only semiquantitative, however due to its simplicity it is used for screening.


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  • Sudan IV — Other names Sudan R, C.I. Solvent Red 24, C.I. 26105, Lipid Crimson, Oil Red, Oil Red BB, Fat Red B, Oil Red IV, Scarlet Red, Scarlet Red N.F, Scarlet Red Scharlach …   Wikipedia

  • Sudan Black B — chembox new ImageFile=Sudan Black B.png ImageSize= IUPACName=(2,2 dimethyl 1,3 dihydroperimidin 6 yl) (4 phenylazo 1 naphthyl)diazene OtherNames= Section1=Chembox Identifiers CASNo= PubChem=61336 SMILES=CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)… …   Wikipedia

  • Sudan III — Chembox new Name = Sudan III ImageFile = Sudan III.png ImageSize = 200px ImageName = Sudan III IUPACName = 1 ((4 (phenyldiazenyl)phenyl)diazenyl)naphthalen 2 ol Section1 = Chembox Identifiers CASNo = 85 83 9 SMILES =… …   Wikipedia

  • Sudan black B fat stain — a stain used to demonstrate Legionella and fat vacuoles in bacterial cells. A heat fixed smear is treated with Sudan black B, cleared with xylol, and counterstained with safranin. Fat vacuoles stain blue black; bacterial cells stain pink …   Medical dictionary

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • Sudan III — A red stain, used for neutral fat in histologic technique; it also stains the fatty envelope of the tubercle bacillus. SYN: Sudan red III. * * * a red fat soluble azo dye that is an important stain for demonstration of neutral fats …   Medical dictionary

  • Sudan brown — A brown stain, derived from α naphthylamine and used as a stain for fats …   Medical dictionary

  • Sudan IV — SYN: scarlet red. * * * Sudan IV fō(ə)r n a red disazo solvent dye used chiefly as a biological stain and in ointments for promoting the growth of epithelium (as in the treatment of burns, wounds, or ulcers) called also scarlet red * * * scarlet… …   Medical dictionary

  • sudan IV — noun also sudan ( s) Usage: usually capitalized S : a red disazo solvent dye used chiefly as a biological stain and in ointments for promoting (as in the treatment of burns, wounds, or ulcers) the growth of epithelium called also scarlet red; see …   Useful english dictionary

  • Sudan black B — A diazo dye, used as a stain for fats …   Medical dictionary

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