Wright's stain

Wright's stain

Wright's stain is a histologic stain that facilitates the differentiation of blood cell types. It is used primarily to stain peripheral blood smears and bone marrow aspirates which are examined under a light microscope. In cytogenetics it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.

It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when infections are expected, and as part of an assessment of overall health.

There are related stains known as the buffered Wright stain, the Wright-Giemsa stain, and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include Eosin Y, Azure B, and Methylene Blue (some commercial preparations combine solutions to simplify staining). The May-Grünwald stain, which produces a more intense coloration, also takes a longer time to perform.

White blood cells stained with Wright's stain:

ee also

*Staining (biology)

External links

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  • Wright's stain — rīts n a stain that is a modification of the Romanowsky stain and is used in staining blood and parasites living in blood Wright James Homer (1869 1928) American pathologist. Wright was a professor of pathology at Harvard Medical School and on… …   Medical dictionary

  • wright's stain — ˈrīts noun Usage: usually capitalized W Etymology: after James Homer Wright died 1928 American pathologist : a stain that is a modification of the Romanowsky stain and is much used in staining blood and blood living parasites …   Useful english dictionary

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  • Wright stain — (rīt) [James Homer Wright, American pathologist, 1869–1928] see Stains and Staining Methods, under stain …   Medical dictionary

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