Dientamoebiasis Classification and external resources ICD-9 007 DiseasesDB 32407 eMedicine ped/563 MeSH D004030
Dientamoebiasis is a medical condition caused by infection with Dientamoeba fragilis. Dientamoeba fragilis is a single celled parasite that infects the lower gastrointestinal tract of humans. It is an important cause of travelers diarrhea, chronic abdominal pain, chronic fatigue and failure to thrive in children.
Early microbiologists reported that the organism was not pathogenic, even though six of the seven individuals from whom they isolated it were experiencing symptoms of dysentery. Their report, published in 1918, concluded the organism was not pathogenic because it consumed bacteria in culture, but did not appear to engulf red blood cells as was seen in the most well known disease causing amoeba of the time, Entamoeba histolytica. This initial report may still be contributing to the reluctance of physicians to diagnose the infection.
As many individuals are asymptomatic carriers of D. fragilis it is proposed there exist a pathogenic and non-pathogenic variants. A study of Dientamoeba fragilis isolates from 60 individuals with symptomatic infection in Sydney Australia found that all were infected with the same genotype. which differed from the North American genotype.
It increases in conditions of crowding and poor sanitation. Rates of infection in military personnel and mental institutions is higher.
The true extent of disease has yet to emerge as most laboratories do not use techniques to adequately identify this organism. An Australian study identified a large number of patients considered to have Irritable bowel syndrome who were actually infected with Dientamoeba fragilis. 
Although Dientamoeba fragilis has been described as an infection that is "emerging from obscurity,"  it has become one of the most prevalent gastrointestinal infections in industrialized countries, especially among children and young adults. A Canadian study reported a prevalence of approximately 10% in boys and girls aged 11–15 years, a prevalence of 11.5% in individuals aged 16–20, and over 20 had a lower incidence of 0.3%-1.9%.
The most commonly reported symptoms in conjunction with infection with Dientamoeba fragilis include abdominal pain (69%) and diarrhea (61%). Diarrhea may be intermittent and may not be present in all cases. It is often chronic - over two weeks in duration. The degree of symptoms may vary from asymptomatic to severe, and can include weight loss, vomiting, fever, and involvement of other digestive organs.
Researchers have reported that symptoms may be more severe in children. Additional symptoms reported have included:
- Weight loss
- Nausea and vomiting
- Urticaria (skin rash)
- Pruritis (itchiness)
- Biliary infection
Organisms similar to Dientamoeba fragilis are known to produce a cyst stage which is able to survive outside of the host and facilitate infection of new hosts. However, the exact manner in which Dientamoeba fragilis is transmitted is not yet known, as scientists have reported that the organism is unable to survive outside its human host for more than a few hours after excretion, and no cyst stage has been found.
Early theories of transmission suggested that Dientamoeba fragilis was unable to produce a cyst stage in infected humans, but some animal existed that in which it did produce a cyst stage, and this animal was responsible for spreading it. However, no such animal has ever been discovered. A later theory suggested the organism was transmitted by pinworms, which provided protection for the parasite outside of the host. Experimental ingestion of pinworm eggs established infection in two investigators. Numerous studies reported high rates of co-infection with helminths  However recent study has failed to show any association between Dientamoeba fragilis infection and pinworm infection. Parasites similar to Dientamoeba fragilis are transmitted by consuming water or food contaminated with feces. The high rate (40%) of concomitant infection with other protazoa reported by at St. Vincent's Hospital, Sydney, Australia. supports the oral fecal route of transmission.
Diagnosis is usually performed by submitting a stool sample for examination by a parasitologist in a procedure known as an Ova and Parasite (O&P) Examination. Approximately 30% of children with D. fragilis infection exhibit peripheral blood eosinophilia.
A minimum of three specimens (stool) should be submitted having been immediately fixed in polyvinyl alcohol fixative, sodium acetate-acetic acid-formalin fixative, or Schaudinn's fixative as the protozoan does not remain morphologically identifiable for long. Further, all specimens, regardless of consistency, should be permanently stained prior to microscopic examination with an oil immersion lens. The disease may remain cryptic due to the lack of a cyst stage if these recommendations are not followed.  Dientamoeba fragilis detection methods and prevalence: a survey of state public health laboratories. J H Grendon, R F Digiacomo, and F J Frost Office of Toxic Substances, Washington State Department of Health, Olympia 98504.
The trophozoite forms have been recovered from formed stool, thus the need to perform the ova and parasite examination on specimens other than liquid or soft stools.
DNA Fragment Analysis provides excellent sensitivity and specificity when compared to microscopy for the detection of D. fragilis and both methods should be employed in laboratories with PCR capability.
The most sensitive detection method is parasite culture, and the culture media require the addition of rice starch.
An indirect fluorescent- antibody (IFA) for fixed stool specimens has been developed.
- One researcher investigated the phenomenon of symptomatic relapse following treatment of infection with Dientamoeba fragilis in association with its apparent disappearance from stool samples. The study found that the organism could still be detected in patients through colonoscopy or by examining stool samples taken in conjunction with a saline laxative.
- A study found that trichrome staining, a traditional method for identification, had a sensitivity of 36% (9/25) when compared to stool culture.
- An additional study found that the sensitivity of staining was 50% (2/4), and that the organism could be successfully cultured in stool specimens up to 12-hours old which were kept at room temperature.
Concomitant Pinworm infection should also be excluded although the association has not been proven.
Successful treatment of the infection with Iodoquinol, Doxycycline, Metronidazole, Paromomycin, and Secnidazole have been reported. Resistance requires the use of combination therapy to eradicate the organism.
All persons living in the same residence should be screened for D. fragilis as asymptomatic carriers may provide a source of repeated infection.
Paromomycin is an effective prophylactic for travellers who will encounter poor sanitation and unsafe drinking water.
- ^ a b c d e Johnson EH, Windsor JJ, Clark CG (2004). "Emerging from obscurity: biological, clinical, and diagnostic aspects of Dientamoeba fragilis". Clin. Microbiol. Rev. 17 (3): 553–70, table of contents. doi:10.1128/CMR.17.3.553-570.2004. PMC 452553. PMID 15258093. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=452553.
- ^ Stark D, Beebe N, Marriott D, Ellis J, Harkness J (2005). "Prospective study of the prevalence, genotyping, and clinical relevance of Dientamoeba fragilis infections in an Australian population". J. Clin. Microbiol. 43 (6): 2718–23. doi:10.1128/JCM.43.6.2718-2723.2005. PMC 1151954. PMID 15956388. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1151954.
- ^ Borody T, Warren E, Wettstein A et al. (2002). "Eradication of Dientamoeba fragilis can resolve IBS-like symptoms". J Gastroenterol Hepatol 17 (Suppl;): A103. doi:10.1046/j.1440-1746.2002.02681.x.
- ^ a b c Lagacé-Wiens PR, VanCaeseele PG, Koschik C (2006). "Dientamoeba fragilis: an emerging role in intestinal disease". Canadian Medical Association Journal 175 (5): 468–9. doi:10.1503/cmaj.060265. PMC 1550747. PMID 16940260. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1550747.
- ^ Vandenberg O, Peek R, Souayah H et al. (2006). "Clinical and microbiological features of dientamoebiasis in patients suspected of suffering from a parasitic gastrointestinal illness: a comparison of Dientamoeba fragilis and Giardia lamblia infections". Int. J. Infect. Dis. 10 (3): 255–61. doi:10.1016/j.ijid.2005.05.011. PMID 16469517.
- ^ a b Norberg A, Nord CE, Evengård B (2003). "Dientamoeba fragilis--a protozoal infection which may cause severe bowel distress". Clin. Microbiol. Infect. 9 (1): 65–8. doi:10.1046/j.1469-0691.2003.00459.x. PMID 12691546.
- ^ Steinitz H, Talis B, Stein B (1970). "Entamoeba histolytica and Dientamoeba fragilis and the syndrome of chronic recurrent intestinal amoebiasis in Israel". Digestion 3 (3): 146–53. doi:10.1159/000197025. PMID 4317789.
- ^ Windsor JJ, Macfarlane L, Hughes-Thapa G, Jones SK, Whiteside TM (2003). "Detection of Dientamoeba fragilis by culture". Br. J. Biomed. Sci. 60 (2): 79–83. PMID 12866914.
- ^ Sawangjaroen N, Luke R, Prociv P (1993). "Diagnosis by faecal culture of Dientamoeba fragilis infections in Australian patients with diarrhoea". Trans. R. Soc. Trop. Med. Hyg. 87 (2): 163–5. doi:10.1016/0035-9203(93)90472-3. PMID 8337717.
- http://emedicine.medscape.com/article/997239-treatment complete diagnostic workup and treatment protocols for adult and pediatric patients; David R Mack, MD, FRCPC, Professor, Departments of Pediatrics and Biochemistry, Microbiology, and Immunology, University of Ottawa Faculty of Medicine
Infectious diseases – Parasitic disease: protozoan infection: Excavata (A06–A07, B55–B57, 007, 085–086) DiscicristataTrypanosomatidaSchizopyrenida TrichozoaTrichomonadida
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