mir-160 microRNA precursor family

mir-160 microRNA precursor family
Predicted secondary structure and sequence conservation of mir-160
Identifiers
Symbol	mir-160
Rfam	RF00247
miRBase	MI0000190
miRBase family	MIPF0000032
Other data
RNA type	Gene; miRNA
Domain(s)	Eukaryota
GO	0035195 0035068
SO	0001244

In molecular biology, mir-160 is a microRNA that has been predicted or experimentally confirmed in a range of plant species including Arabidopsis thaliana (mouse-ear cress) and Oryza sativa (rice).^[1] miR-160 is predicted to bind complementary sites in the untranslated regions of auxin response factor genes to regulate their expression.^[2] The hairpin precursors (represented here) are predicted based on base pairing and cross-species conservation; their extents are not known. In this case, the mature sequence is excised from the 5' arm of the hairpin.

Specifically, 3 of A. thaliana's 23 auxin-response factor genes are thought to be post-transcriptionally regulated by mir-160.^[3][4] When one of these targets (ARF17) is manipulated to become miRNA-resistant, several developmental defects can be observed in the host plant.^[3] This experiment has been repeated with another mir-160 target, ARF10, and results highlighted a regulatory role in post-embryonic development and seed germination.^[5]

References

1. ^ "miRNA gene family: MIR160". mirBASE. University of Manchester. http://microrna.sanger.ac.uk/cgi-bin/sequences/mirna_summary.pl?fam=MIPF0000032. Retrieved 5 September 2011. 
2. ^ Rhoades, MW; Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP (2002). "Prediction of plant microRNA targets". Cell 110 (4): 513–520. doi:10.1016/S0092-8674(02)00863-2. PMID 12202040. 
3. ^ ^{a b} Mallory, AC; Bartel, DP, Bartel, B (2005 May). "MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes.". The Plant cell 17 (5): 1360–75. PMID 15829600. 
4. ^ Liu, X; Huang, J, Wang, Y, Khanna, K, Xie, Z, Owen, HA, Zhao, D (2010 May). "The role of floral organs in carpels, an Arabidopsis loss-of-function mutation in MicroRNA160a, in organogenesis and the mechanism regulating its expression.". The Plant journal : for cell and molecular biology 62 (3): 416–28. PMID 20136729. 
5. ^ Liu, PP; Montgomery, TA, Fahlgren, N, Kasschau, KD, Nonogaki, H, Carrington, JC (2007 Oct). "Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages.". The Plant journal : for cell and molecular biology 52 (1): 133–46. PMID 17672844. 

External links

• Rfam page for mir-160 microRNA precursor family
• MIPF0000032