NCK adaptor protein 1

PDB rendering based on 2ci8.
Symbols NCK1; MGC12668; NCK; NCKalpha; nck-1
External IDs OMIM600508 MGI109601 HomoloGene38148 GeneCards: NCK1 Gene
RNA expression pattern
PBB GE NCK1 204725 s at tn.png
PBB GE NCK1 211063 s at tn.png
More reference expression data
Species Human Mouse
Entrez 4690 17973
Ensembl ENSG00000158092 ENSMUSG00000032475
UniProt P16333 n/a
RefSeq (mRNA) NM_001190796.1 NM_010878.2
RefSeq (protein) NP_001177725.1 NP_035008.2
Location (UCSC) Chr 3:
136.58 – 136.67 Mb
Chr 9:
100.39 – 100.45 Mb
PubMed search [1] [2]

Cytoplasmic protein NCK1 is a protein that in humans is encoded by the NCK1 gene.[1][2]

The Nck(non-catalytic region of tyrosine kinase adaptor protein 1)Protein belongs to the adaptor family of proteins.The nck gene was initially isolated from a human melanoma cDNA library using a monoclonal antibody produced against the human melanoma-associated antigen. The Nck family has two known members in human cells (Nck-1/Nckalpha and NcK2/NcKbeta), two in mouse cells (mNckalpha and mNckbeta/Grb4) and one in drosophila (Dock means dreadlocks-ortholog). The two murine gene products exhibit 68% aminoacid identity to one another,with most of the sequence variation being located to the linker regions between the SH3 and SH2 domains, and are 96% identical to their human counterparts. While human nck-1 gene has been localised to the 3q21 locus of chromosome 3, the nck-2 gene can be found on chromosome 2 at the 2q12 locus. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.[3]



NCK1 has been shown to interact with DNM1,[4] EIF2B2,[5] KHDRBS1,[6][7] Lymphocyte cytosolic protein 2,[8][9] EPH receptor B1,[10] SOCS7,[11] MINK1,[12] MAP4K1,[13][14][15] MAP4K4,[16] Abl gene,[17][18] WIPF1,[19] RRAS,[20] Wiskott-Aldrich syndrome protein,[21][22][23] Epidermal growth factor receptor,[6][24][25] PDGFRB,[24][25] PTK2,[26][27] NCKIPSD,[28] RICS,[29] Cbl gene,[17][30] TANK-binding kinase 1,[31] SOS1,[4][25][32][33] Dystroglycan,[34] PAK1,[25][35][36] PKN2,[25][37] NEDD9,[26] WASL[38] and RASA1.[39]

See also


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  3. ^ "Entrez Gene: NCK1 NCK adaptor protein 1". 
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  5. ^ Kebache, Sem; Zuo Dongmei, Chevet Eric, Larose Louise (Apr. 2002). "Modulation of protein translation by Nck-1". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (8): 5406–11. doi:10.1073/pnas.082483399. ISSN 0027-8424. PMC 122782. PMID 11959995. 
  6. ^ a b Tang, J; Feng G S, Li W (Oct. 1997). "Induced direct binding of the adapter protein Nck to the GTPase-activating protein-associated protein p62 by epidermal growth factor". Oncogene (ENGLAND) 15 (15): 1823–32. doi:10.1038/sj.onc.1201351. ISSN 0950-9232. PMID 9362449. 
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  8. ^ Shim, Eun Kyung; Moon Chang Suk, Lee Gi Yeon, Ha Yun Jung, Chae Suhn-Kee, Lee Jong Ran (Sep. 2004). "Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase". FEBS Lett. (Netherlands) 575 (1–3): 35–40. doi:10.1016/j.febslet.2004.07.090. ISSN 0014-5793. PMID 15388330. 
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  18. ^ Ren, R; Ye Z S, Baltimore D (Apr. 1994). "Abl protein-tyrosine kinase selects the Crk adapter as a substrate using SH3-binding sites". Genes Dev. (UNITED STATES) 8 (7): 783–95. doi:10.1101/gad.8.7.783. ISSN 0890-9369. PMID 7926767. 
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  20. ^ Wang, B; Zou J X, Ek-Rylander B, Ruoslahti E (Feb. 2000). "R-Ras contains a proline-rich site that binds to SH3 domains and is required for integrin activation by R-Ras". J. Biol. Chem. (UNITED STATES) 275 (7): 5222–7. doi:10.1074/jbc.275.7.5222. ISSN 0021-9258. PMID 10671570. 
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  22. ^ Okabe, Seiichi; Fukuda Seiji, Broxmeyer Hal E (Jul. 2002). "Activation of Wiskott-Aldrich syndrome protein and its association with other proteins by stromal cell-derived factor-1alpha is associated with cell migration in a T-lymphocyte line". Exp. Hematol. (Netherlands) 30 (7): 761–6. doi:10.1016/S0301-472X(02)00823-8. ISSN 0301-472X. PMID 12135674. 
  23. ^ Rivero-Lezcano, O M; Marcilla A, Sameshima J H, Robbins K C (Oct. 1995). "Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains". Mol. Cell. Biol. (UNITED STATES) 15 (10): 5725–31. ISSN 0270-7306. PMC 230823. PMID 7565724. 
  24. ^ a b Li, W; Hu P, Skolnik E Y, Ullrich A, Schlessinger J (Dec. 1992). "The SH2 and SH3 domain-containing Nck protein is oncogenic and a common target for phosphorylation by different surface receptors". Mol. Cell. Biol. (UNITED STATES) 12 (12): 5824–33. ISSN 0270-7306. PMC 360522. PMID 1333047. 
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  26. ^ a b Minegishi, M; Tachibana K, Sato T, Iwata S, Nojima Y, Morimoto C (Oct. 1996). "Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes". J. Exp. Med. (UNITED STATES) 184 (4): 1365–75. doi:10.1084/jem.184.4.1365. ISSN 0022-1007. PMC 2192828. PMID 8879209. 
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  39. ^ Ger, M; Zitkus Z, Valius M (Oct. 2011). "Adaptor protein Nck1 interacts with p120 Ras GTPase-activating protein and regulates its activity". Cell. Signal. (ENGLAND) 23 (10): 1651–8. doi:10.1016/j.cellsig.2011.05.019. ISSN 0898-6568. PMID 21664272. 

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