DNA ligase

DNA ligase
DNA ligase
DNA Repair.jpg
DNA ligase repairing chromosomal damage
EC number
CAS number 9015-85-4
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO
ligase I, DNA, ATP-dependent
DNA Ligase.jpg
Symbol LIG1
Entrez 3978
HUGO 6598
OMIM 126391
RefSeq NM_000234
UniProt P18858
Other data
Locus Chr. 19 [1]
ligase III, DNA, ATP-dependent
Symbol LIG3
Entrez 3980
HUGO 6600
OMIM 600940
RefSeq NM_002311
UniProt P49916
Other data
Locus Chr. 17 q11.2-q12
ligase IV, DNA, ATP-dependent
Symbol LIG4
Entrez 3981
HUGO 6601
OMIM 601837
RefSeq NM_002312
UniProt P49917
Other data
Locus Chr. 13 q33-q34

In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC that repairs single-stranded discontinuities in double stranded DNA molecules, in simple words strands that have double-strand break (a break in both complementary strands of DNA). Purified DNA ligase is used in gene cloning to join DNA molecules together. The alternative, a single-strand break, is fixed by a different type of DNA ligase using the complementary strand as a template,[1] but still requires DNA ligase to create the final phosphodiester bond to fully repair the DNA.

DNA ligase has applications in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for Genetic recombination experiments (see Applications in molecular biology research).


Ligase mechanism

The mechanism of DNA ligase is to form two covalent phosphodiester bonds between 3' hydroxyl ends of one nucleotide, ("acceptor") with the 5' phosphate end of another ("donor"). ATP is required for the ligase reaction, which proceeds in three steps: (1) adenylation (addition of AMP) of a residue in the active center of the enzyme, pyrophosphate is released; (2) transfer of the AMP to the 5' phosphate of the so-called donor, formation of a pyrophosphate bond; (3) formation of a phosphodiester bond between the 5' phosphate of the donor and the 3' hydroxyl of the acceptor.[2]

A pictorial example of how a ligase works (with sticky ends)

Ligase will also work with blunt ends, although higher enzyme concentrations and different reaction conditions are required.

Mammalian ligases

In mammals, there are four specific types of ligase.

Some forms of DNA ligase present in bacteria (usually larger) may require NAD to act as a co-factor, whereas other forms of DNA ligases (usually present in E.coli, and usually smaller) may require ATP to react. Also, a number of other structures present in the DNA ligase are the AMP and lysine, both of which are important in the ligation process since they create an intermediate enzyme.

Applications in molecular biology research

DNA ligases have become an indispensable tool in modern molecular biology research for generating recombinant DNA sequences. For example, DNA ligases are used with restriction enzymes to insert DNA fragments, often genes, into plasmids.

One vital aspect to performing efficient recombination experiments involving the ligation of cohesive-ended fragments is controlling the optimal temperature. Most experiments use T4 DNA Ligase (isolated from bacteriophage T4), which is most active at 25°C. However, for optimal ligation efficiency with cohesive-ended fragments ("sticky ends"), the optimal enzyme temperature needs to be balanced with the melting temperature Tm (also the annealing temperature) of the sticky ends being ligated.[3] If the ambient temperature exceeds Tm, the homologous pairing of the sticky ends would not be stable because the high temperature disrupts hydrogen bonding.[4] Ligation reaction is most efficient when the sticky ends are already stably annealed, disruption of the annealing ends would therefore results in low ligation efficiency. The shorter the overhang, the lower the Tm, typically a 4-base overhang has a Tm of 12-16°C.

Since blunt-ended DNA fragments have no cohesive ends to anneal, the melting temperature is not a factor to consider within the normal temperature range of the ligation reaction. However, the higher the temperature, the less chance that the ends to be joined will be aligned to allow ligation (molecules move around the solution more at higher temperatures). The limiting factor in blunt end ligation is not the activity of the ligase but rather the number of alignments between DNA fragment ends that occur. The most efficient ligation temperature for blunt-ended DNA would therefore be the temperature at which the greatest number of alignments can occur. Therefore, the majority of blunt-ended ligations are carried out at 14-16°C overnight. The absence of a stably annealed ends also means that the ligation efficiency is lowered, requiring a higher ligase concentration to be used.(T4 DNA ligase is the only commercially-available DNA ligase to anneal blunt ends).[3]


The first DNA ligase was purified and characterized in 1967. The common commercially available DNA ligases were originally discovered in bacteriophage T4, E. coli and other bacteria.[5]

See also


  1. ^ Pascal, John M.; O'Brien, Patrick J.; Tomkinson, Alan E.; Ellenberger, Tom (2004). "Human DNA ligase I completely encircles and partially unwinds nicked DNA". Nature 432 (7016): 473–8. doi:10.1038/nature03082. PMID 15565146. 
  2. ^ Lehnman, I. R. (1974). "DNA Ligase: Structure, Mechanism, and Function". Science 186 (4166): 790–7. doi:10.1126/science.186.4166.790. PMID 4377758. 
  3. ^ a b Tabor, Stanley. DNA ligases. Chapter in: Current Protocols in Molecular Biology, Book 1. 2001: Wiley Interscience.[page needed]
  4. ^ Law-breaking liquid defies the rules at physicsworld.com
  5. ^ Weiss, B.; Richardson, CC (1967). "Enzymatic Breakage and Joining of Deoxyribonucleic Acid, I. Repair of Single-Strand Breaks in DNA by an Enzyme System from Escherichia coli Infected with T4 Bacteriophage". Proceedings of the National Academy of Sciences 57 (4): 1021–8. doi:10.1073/pnas.57.4.1021. PMC 224649. PMID 5340583. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=224649. 

External links

Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • DNA-Ligase — Bezeichner Externe IDs CAS Nummer:&# …   Deutsch Wikipedia

  • DNA ligase — ligase ligase n. (Biochem.) An enzyme which catalyzes creation of a covalent bond between two substrates, resulting in a larger product which is a combination of the two starting materials; especially, {DNA ligase}, an enzyme which creates a… …   The Collaborative International Dictionary of English

  • DNA ligase — DNA li·gase (liґgās) an enzyme of the ligase class that catalyzes the energy driven linkage of a double stranded DNA chain with a free 3′ hydroxyl group to one with a 5′ phosphate group, forming a phosphodiester bond between them …   Medical dictionary

  • DNA-Ligase — DNA Ligase,   Molekulargenetik: Enzym, das DNA Fragmente unter Ausbildung einer Esterbindung zwischen Phosphorsäure und Desoxyribose verknüpft. DNA L. spielt v. a. eine Rolle bei der Reparatur von DNA Schäden (DNA Reparatur) sowie bei der… …   Universal-Lexikon

  • DNA ligase — Enzyme involved in DNA replication. The DNA ligase of E. coliseals nicks in one strand of double stranded DNA, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand. Nicks are breaks in the… …   Dictionary of molecular biology

  • DNA ligase — DNR ligazė statusas T sritis chemija apibrėžtis Fermentas, sujungiantis viengrandės DNR fragmentus fosfodiesteriniu ryšiu esant komplementariai DNR matricai. atitikmenys: angl. DNA ligase rus. ДНК лигаза …   Chemijos terminų aiškinamasis žodynas

  • DNA ligase — An enzyme that catalyses a reaction to link two separate DNA molecules via the formation of a phosphodiester bond between the 3 hydroxyl end of one and the 5 phosphate of the other. Its natural role lies in DNA repair and replication. An… …   Glossary of Biotechnology

  • DNA ligase — An enzyme that joins two DNA fragments together through the formation of a new phosphodiester bond …   Dictionary of microbiology

  • DNA ligase —    An enzyme that catalyzes nucleotide phisphodiester bond formation …   Forensic science glossary

  • DNA-Replikation — DNA Replikation. Die Doppelhelix wird durch die Helicase und die Topoisomerase geöffnet. Danach setzt die Primase einen Primer und die …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”