Sodium orthovanadate

Sodium orthovanadate
IUPAC name Sodium vanadate(V)
Other names sodium vanadate (V), sodium vanadium oxide
Identifiers
CAS number	13721-39-6 Y
PubChem	61671
RTECS number	YW1120000
Properties
Molecular formula	Na3VO4
Molar mass	183.908 g/mol
Appearance	white powder
Density	2.16 g/cm³, solid
Melting point	858 °C
Solubility in water	soluble
Hazards
Main hazards	Harmful.
NFPA 704	1 2 0
Flash point	Non-flammable
Y orthovanadate (verify) (what is: Y/N?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Sodium orthovanadate is the chemical compound Na₃VO₄ and contains the tetrahedral VO₄³⁻.^[1] It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO₄^3- ion binds reversibly to the active sites of most protein tyrosine phosphatases.

It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final working concentration of 1 - 10 μM. It is toxic when inhaled, swallowed or in contact with skin.

References

1. ^ Greenwood, Norman N.; Earnshaw, Alan (1997). Chemistry of the Elements (2nd ed.). Oxford: Butterworth-Heinemann. ISBN 0080379419. 

Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982).

See also

• Sodium metavanadate