Mucin

Mucin
Micrograph showing cells with prominent mucin-containing intracytoplasmic vacuoles. Pap stain.

Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most metazoans.[1] Mucins' key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers.[1] They often take an inhibitory role.[1] Some mucins are associated with controlling mineralization, including nacre formation in molluscs,[2] calcification in echinoderms[3] and bone formation in vertebrates.[4] They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1 is associated with many types of cancer.[5]

Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted onto mucosal surfaces or secreted to become a component of saliva.

Contents

Genes

At least 19 human mucin genes have been distinguished by cDNA cloning — MUC1, MUC2, MUC3A, MUC3B, MUC4, MUC5AC, MUC5B, MUC6, MUC7, MUC8, MUC12, MUC13, MUC15, MUC16, MUC17, MUC19, and MUC20.[6]

The major secreted airway mucins are MUC5AC and MUC5B, while MUC2 is secreted mostly in the intestine but also in the airway.

Protein structure

Mature mucins are composed of two distinct regions:

  • The amino- and carboxy-terminal regions are very lightly glycosylated, but rich in cysteines. The cysteine residues participate in establishing disulfide linkages within and among mucin monomers.
  • A large central region formed of multiple tandem repeats of 10 to 80 residue sequences in which up to half of the amino acids are serine or threonine. This area becomes saturated with hundreds of O-linked oligosaccharides. N-linked oligosaccharides are also found on mucins, but in less abundance than O-linked sugars.

Glycosylation and aggregation

Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by exceptionally abundant glycosylation.

The dense "sugar coating" of mucins gives them considerable water-holding capacity and also makes them resistant to proteolysis, which may be important in maintaining mucosal barriers.

Mucins are secreted as massive aggregates of proteins with molecular masses of roughly 1 to 10 million Da. Within these aggregates, monomers are linked to one another mostly by non-covalent interactions, although intermolecular disulfide bonds may also play a role in this process.

Secretion

Upon stimulation, MARCKS (myristylated alanine-rich C kinase substrate) protein coordinates the secretion of mucin from mucin filled vesicles within the specialized epithelial cells.[7] Fusion of the vesicles to the plasma membrane causes release of the mucin, which as it exchanges Ca2+ for Na+ expands up to 600 fold. The result is a viscoelastic product of interwoven molecules which, combined with other secretions (e.g., from the airway epithelium and the submucosal glands in the respiratory system), is called mucus.[8] [9]

Clinical significance

Increased mucin production occurs in many adenocarcinomas, including cancers of the pancreas, lung, breast, ovary, colon and other tissues. Mucins are also overexpressed in lung diseases such as asthma, bronchitis, COPD or cystic fibrosis. Two membrane mucins, MUC1 and MUC4 have been extensively studied in relation to their pathological implication in the disease process.[10][11][12] Mucins are under investigation as possible diagnostic markers for malignancies and other disease processes in which they are most commonly over- or mis-expressed.

Abnormal deposits of mucin are responsible for the non-pitting facial edema seen in untreated hypothryoidism. This edema is seen in the pretibial area as well.[13]

References

  1. ^ a b c Marin, F.; Luquet, G.; Marie, B.; Medakovic, D. (2007). "Molluscan Shell Proteins: Primary Structure, Origin, and Evolution". Current Topics in Developmental Biology Volume 80. Current Topics in Developmental Biology. 80. pp. 209. doi:10.1016/S0070-2153(07)80006-8. ISBN 9780123739148.  edit
  2. ^ Marin, F.; Corstjens, P.; De Gaulejac, B.; De Vrind-De Jong, E.; Westbroek, P. (2000). "Mucins and molluscan calcification. Molecular characterization of mucoperlin, a novel mucin-like protein from the nacreous shell layer of the fan mussel Pinna nobilis (Bivalvia, pteriomorphia)". The Journal of biological chemistry 275 (27): 20667–20675. doi:10.1074/jbc.M003006200. PMID 10770949.  edit
  3. ^ Boskey, A. (2003). "Biomineralization: an Overview". Connective Tissue Research 44 (1): 5–9. doi:10.1080/713713622. PMID 12952166.  edit
  4. ^ RJ Midura, VC Hascall (1996). "Bone sialoprotein–a mucin in disguise?". Glycobiology 6 (7): 677–81. doi:10.1093/glycob/6.7.677. PMID 8953277. 
  5. ^ Niv Y (April 2008). "MUC1 and colorectal cancer pathophysiology considerations". World J. Gastroenterol. 14 (14): 2139–41. doi:10.3748/wjg.14.2139. PMC 2703837. PMID 18407586. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2703837. 
  6. ^ Perez-Vilar, J; Hill, RL (2004). "Mucin Family of Glycoproteins". Encyclopedia of Biological Chemistry (Lennarz & Lane, EDs.) (Oxford: Academic Press/Elsevier) 2: 758–764. 
  7. ^ Li, Y; Martin, LD; Spizz, G; Adler, KB (November 2, 2001). "MARCKS protein is a key molecule regulating mucin secretion by human airway epithelial cells in vitro". J Biol Chem 276 (44): 40982–90. doi:10.1074/jbc.M105614200. PMID 11533058. 
  8. ^ Rogers, DF (September 2007). "Physiology of airway mucus secretion and pathophysiology of hypersecretion". Respir Care 52 (9): 1134–1146. PMID 17716382. 
  9. ^ Perez-Vilar, J (20087). "Mucin granule intraluminal organization". Am J Respir Cell Mol Biol 36 (2): 183–190. doi:10.1165/rcmb.2006-0291TR. PMC 2176109. PMID 16960124. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2176109. 
  10. ^ Singh AP, Moniaux N, Chauhan SC, Meza JL, Batra SK (January 2004). "Inhibition of MUC4 expression suppresses pancreatic tumor cell growth and metastasis.". Cancer research 64 (2): 622–30. PMID 14744777. 
  11. ^ Singh Ajay P., Chauhan Subhash C., Bafna Sangeeta, Johansson Sonny L., Smith Lynette M., Moniaux Nicolas, Lin Ming-Fong, Batra Surinder K. (March 2006). "Aberrant expression of transmembrane mucins, MUC1 and MUC4, in human prostate carcinomas". The Prostate 66 (4): 421–429. doi:10.1002/pros.20372. PMID 16302265. 
  12. ^ Singh A. P., Chaturvedi P., Batra S. K. (January 2007). "Emerging Roles of MUC4 in Cancer: A Novel Target for Diagnosis and Therapy". Cancer Research 67 (2): 433–436. doi:10.1158/0008-5472.CAN-06-3114. PMID 17234748. 
  13. ^ Hanberg, Allen "Medical Surgical Nursing: clinical management for positive outcomes" Black and Hawk (Eds.). ElSevier 2009.
  • Ali M, Hutton D, Wilson J, Pearson J (September 2005). "Major Secretory Mucin Expression in Chronic Sinusitis". Otolaryngology - Head and Neck Surgery 133 (3): 423–428. doi:10.1016/j.otohns.2005.06.005. PMID 16143194. 

External links



Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Mucin-1 — Masse/Länge Primärstruktur 1232 = 1074+158 Aminosäuren …   Deutsch Wikipedia

  • Mučín — (Village) Administration Pays  Slovaquie …   Wikipédia en Français

  • Mucin — Mu cin (m[=u] s[i^]n), n. [From {Mucus}.] 1. (Bot. Chem.) See {Mucedin}. [Obs.] [1913 Webster] 2. (Physiol. Chem.) Any of a class of high molecular weight glycoproteins which are contained in mucus, and give to the latter secretion its peculiar… …   The Collaborative International Dictionary of English

  • Mucīn — Mucīn, 1) nach Saussure ein Bestandtheil des rohen Klebers; man erhält es, indem man den rohen Kleber mit Alkohol auskocht, aus der alkoholischen Lösung den Pflanzenleim fällt u. die wässerige Flüssigkeit verdunstet; 2) so v.w. Diastase; 3)… …   Pierer's Universal-Lexikon

  • Mucīn — Mucīn, s. Schleim …   Meyers Großes Konversations-Lexikon

  • mucin — [myo͞o′sin] n. [Fr mucine: see MUCUS & IN1] any of various glycoproteins in connective tissue, saliva, mucus, etc., that lubricate and protect the body mucinoid adj. mucinous …   English World dictionary

  • Mucin — Muzine (engl. Mucins, von lat. mucus = „Schleim“), sind der strukturgebende Bestandteil des Schleims auf den Schleimhäuten (Mucosa). Muzine sind Glykoproteine, also Makromoleküle aus einer zentralen Proteinkette und langen Seitenketten aus… …   Deutsch Wikipedia

  • Mučín — Location of Lučenec District in the Banská Bystrica Region Mučín (Hungarian: Mucsény) is a village and municipality in the Lučenec District in the Banská Bystrica Region of Slovakia. External links …   Wikipedia

  • mucin — A secretion containing carbohydrate rich glycoproteins such as that from the goblet cells of the intestine, the submaxillary glands, and other mucous glandular cells; it is also present in the ground substance of connective tissue, especially… …   Medical dictionary

  • mucin — Mucedin Mu*ce din, n. [From {Mucus}.] (Bot. Chem.) A yellowish white, amorphous, nitrogenous substance found in wheat, rye, etc., and resembling gluten; formerly called also {mucin}. [1913 Webster] …   The Collaborative International Dictionary of English

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”