Nucleoside phosphoramidite

Protected 2'-deoxynucleoside phosphoramidites.

Nucleoside phosphoramidites are derivatives of natural or synthetic nucleosides. They are used to synthesize oligonucleotides, relatively short fragments of nucleic acid and their analogs. Nucleoside phosphoramidites were first introduced in 1981 by Beaucage and Caruthers.^[1] In order to avoid undesired side reactions, reactive hydroxy and exocyclic amino groups present in natural or synthetic nucleosides are appropriately protected. As long as a nucleoside analog contains at least one hydroxy group, the use of the appropriate protecting strategy allows one to convert that to the respective phosphoramidite and to incorporate the latter into synthetic nucleic acids. In order to be incorporated in the middle of an oligonucleotide chain using phosphoramidite strategy, the nucleoside analog have to possess two hydroxy groups or, less often, a hydroxy group and another nucleophilic group (amino or mercapto). Examples include, but are not limited to, alternative nucleotides, LNA, morpholino, nucleosides modified at the 2'-position (OMe, protected NH₂, F), nucleosides containing non-canonical bases (hypoxanthine and xanthine contained in natural nucleosides inosine and xanthosine, respectively, tricyclic bases such as G-clamp,^[2] etc.) or bases derivatized with a fluorescent group or a linker arm.

Preparation of nucleoside phosphoramidites

There are three main methods for the preparation of nucleoside phosphoramidites.

• 

  DMT = 4,4'-dimethoxytrityl; B = optionally protected nucleic base; R = phosphate protecting group
  The method used most commonly consist in the treatment of a protected nucleoside bearing a single free hydroxy group with phosphorodiamidite under the catalytic action of a weak acid.^[3][4] Although some bisamidites were reported as thermally unstable compounds,^[5] 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite, the amidite used for the preparation of commercial nucleoside phosphoramidites, is relatively stable. It can be synthesized using a two-step, one-pot procedure and purified by vacuum distillation.^[6] An excellent review outlines the use of the latter reagent in preparation of nucleosidic and non-nucleosidic phosphoramidites in great detail.^[7]

• 
  In the second method, the protected nucleoside is treated with the phosphorochloridite in the presence of an organic base, most commonly N-ethyl-N,N-diisopropylamine (Hunig's base).^[8]

• 
  In the third method,^[9] the protected nucleoside is first treated with chloro N,N,N',N'-tetraisopropyl phosphorodiamidite in the presence of an organic base, most commonly N-ethyl-N,N-diisopropylamine (Hunig's base) to form a protected nucleoside diamidite. The latter is treated with an alcohol respective to the desired phosphite protecting group, for instance, 2-cyanoethanol, in the presence of a weak acid.

Nucleoside phosphoramidites are purified by column chromatography on silica gel. To warrant the stability of the phosphoramidite moiety, it is advisable to maintain 3 to 5% concentration of a weak base (triethylamine or pyridine) in the eluent throughout the entire course of the separation. The purity of a phosphoramidite may be assessed by ³¹P NMR spectroscopy. As the P(III) atom is chiral, in a nucleoside phosphoramidite it displays two peaks at about 149 ppm corresponding to the two diastereomers of the compound. The potentially present H-phosphonate impurities display peaks at 8 and 10 ppm.

Chemical properties of phosphoramidite moiety

Nucleoside phosphoramidites are relatively stable compounds with a prolonged shelf-life when stored as powders under anhydrous conditions in the absence of air at temperatures below 4^oC. The amidites well withstand mild basic conditions. In contrast, in the presence of even mild acids, phosphoramidites perish almost instantaneously. The phosphoramidites are relatively stable to hydrolysis under neutral conditions. For instance, half-life of 2-cyanoethyl 5'-O-(4,4'-dimethoxytrityl)thymidine-3'-O-(N,N-diisopropylamino)phosphite in 95% aqueous acetonitrile at 25^oC is 200 h.^[10]

• 

  X = O, S, NH.
  The most important feature of phosphoramidites is their ability to undergo the phosphoramidite coupling reaction that is, to react with nucleophilic groups in the presence of an acidic azole catalyst, 1H-tetrazole, 2-ethylthiotetrazole,^[11] 2-benzylthiotetrazole,^[12][13] 4,5-dicyanoimidazole,^[14] or a number of similar compounds. The reaction proceeds extremely rapidly. This very feature makes nucleoside phosphoramidites useful intermediates in oligonucleotide synthesis. Stereochemically, the phosphoramidite coupling leads to the epimerisation (forming of diastereomers) at the P(III) chiral center.

When water is served as a nucleophile, the product is an H-phosphonate diester as shown in Scheme above. Due to the presence of residual water in solvents and reagents, the formation of the latter compound is the most common complication in the preparative use of phosphoramidites, particularly in oligonucleotide synthesis.

• 

  X = S, Se.
  Phosphoramidites are readily oxidized with weak oxidating reagents, for instance, with aqueous iodine in the presence of weak bases or with hydrogen peroxide^[15] to form the respective phosphoramidates.

Similarly, phosphoramidites react with other chalcogens. When brought in contact with a solution of sulfur^[15][16] or a number of compounds collectively referred to as sulfurizing agents,^[17][18] phosphoramidites quantitatively form phosphorothioamidates. The reaction with selenium^[15][16] or selenium derivatives^[19] produces phosphoroselenoamidates. In all reactions of this type, the configuration at the phosphorus atom is retained.

• 
  Nucleoside phosphoramidites undergo Michaelis-Arbuzov reaction to form the respective phosphonamidates. One example describes the preparation of phosphonamidates in the presence of acrylonitrile.^[20] Reportedly,, at room temperature the reaction is stereoselective with the retention of configuration at the phosphorus center. In contrast, when carried out ar 55^oC, the reaction leads to racemized products.

• Similarly to phosphines and tertiary phosphites, phosphoramidites readily undergo Staudinger reaction.

(RO)₂P-N(R¹)₂ + R²-N₃ + H₂O ---- (RO)₂P(=O)-N(R¹)₂ + R²-NH₂ + N₂;

Protecting strategy

The naturally occurring nucleotides (nucleoside-3'- or 5'-phosphates) and their phosphodiester analogs are insufficiently reactive to afford an expedite synthetic preparation of oligonucleotides in high yields. The selectivity and the rate of the formation of internucleosidic linkages are dramatically improved by using 3'-O-(N,N-diisopropyl phosphoramidite) derivatives of nucleosides (nucleoside phosphoramidites) that serve as building blocks in phosphite triester methodology. To prevent undesired side reactions, all other functional groups present in nucleosides have to be rendered unreactive (protected) by attaching protecting groups. Upon the completion of the oligonucleotide chain assembly, all the protecting groups are removed to yield the desired oligonucleotides. Below, the protecting groups currently used in commercially available^{[21][22][23][24][25]} and most common nucleoside phosphoramidite building blocks are briefly reviewed:

• The 5'-hydroxyl group is protected by an acid-labile DMT (4,4'-dimethoxytrityl) group.

• Thymine and uracil, nucleic bases of thymidine and uridine, respectively, do not have exocyclic amino groups and hence do not require any protection. In contrast, nucleic bases adenine, cytosine, and guanine bear the exocyclic amino groups, which are reactive with the activated phosphoramidites under the conditions of the coupling reaction. Although, at the expense of additional steps in the synthetic cycle, the oligonucleotide chain assembly may be carried out using phosphoramidites with unprotected amino groups,^[26] most often these are kept permanently protected over the entire length of the oligonucleotide chain assembly. The protection of the exocyclic amino groups have to be orthogonal to that of the 5'-hydroxy group because the latter is removed at the end of each synthetic cycle. The simplest to implement and hence the most widely accepted is the strategy where the exocyclic amino groups bear a base-labile protection. Most often, two protection schemes are used.

• In the first, the standard and more robust scheme (Figure), Bz (benzoyl) protection is used for A, dA, C, dC, G, and dG are protected with isobutyryl group. More recently, Ac (acetyl) group is often used to protect C and dC as shown in Figure.^[27]

• In the second, mild protection scheme, A and dA are protected with isobutyryl^[28] or phenoxyacetyl groups (PAC).^[29] C and dC bear acetyl protection,^[27] and G and dG are protected with 4-isopropylphenoxyacetyl (i-Pr-PAC)^[30] or dimethylformamidino (dmf)^[31] groups. Mild protecting groups are removed more readily than the standard protecting groups. However, the phosphoramidites bearing these groups are less stable when stored in solution.

• The phosphite group is protected by a base-labile 2-cyanoethyl group.^[32] Once a phosphoramidite has been coupled to the solid support-bound oligonucleotide and the phosphite moieties have been converted to the P(V) species, the presence of the phosphate protection is not mandatory for the successful conducting of further coupling reactions.^[33]

2'-O-Protected ribonucleoside phosphoramidites.

• In RNA synthesis, the 2'-hydroxy group is protected with TBDMS (t-butyldimethylsilyl) group.^{[34][35][36][37]} or with TOM (tri-iso-propylsilyloxymethyl) group,^[38][39] both being removable by treatment with fluoride ion.

• The phosphite moiety also bears a diisopropylamino (iPr₂N) group reactive under acidic conditions. Upon activation, the diisopropylamino group leaves to be substituted by the 5'-hydroxy group of the support-bound oligonucleotide.

References

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2. ^ Lin, K.-Y., Matteucci, M. D. (1998). "A cytosine analog capable of clamp-like binding to a guanine in helical nucleic acids". J. Amer. Chem. Soc. 120 (33): 8531–8532. doi:10.1021/ja981286z. 
3. ^ Nielsen, J.; Marugg, J. E.; Taagaard, M.; Van Boom, J. H.; Dahl, O. (1986). "Polymer-supported synthesis of deoxyoligonucleotides using in situ prepared deoxynucleoside 2-cyanoethyl phosphoramidites". Rec. Trav. Chim. Pays-Bas 105 (1): 33–34. 
4. ^ Nielsen, J.; Taagaard, M.; Marugg, J. E.; Van Boom, J. H.; Dahl, O. (1986). "Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides". Nucl. Acids Res. 14 (18): 7391–7403. doi:10.1093/nar/14.18.7391. 
5. ^ Nielsen, J.; Marugg, J. E.; Van Boom, J. H.; Honnens, J.; Taagaard, M.; Dahl, O. (1986). "Thermal instability of some alkyl phosphorodiamidites". J. Chem Res. Synopses (1): 26–27. 
6. ^ Nielsen, J.; Dahl, O. (1987). "Improved synthesis of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite (iPr2N)2POCH2CH2CN)". Nucl. Acids Res. 15 (8): 3626. doi:10.1093/nar/15.8.3626. 
7. ^ Beaucage, S. L. (2001). "2-Cyanoethyl Tetraisopropylphosphorodiamidite". e-EROS Encyclopedia of Reagents for Organic Synthesis. doi:10.1002/047084289X.rn00312. 
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17. ^ Wilk, A.; Uznanski, B.; Stec, W. J. (1991). "Assignment of absolute configuration at phosphorus in dithymidylyl(3',5')phosphormorpholidates and -phosphormorpholidothioates.". Nucleosides & Nucleotides 10 (1-3): 319–322. 
18. ^ Guzaev, A. P. (2011). "Reactivity of 3H-1,2,4-dithiazole-3-thiones and 3H-1,2-dithiole-3-thiones as sulfurizing agents for oligonucleotide synthesis". Tetrahedron Letters 52: 434–437. doi:10.1016/j.tetlet.2010.11.086. 
19. ^ Holloway, G. A.; Pavot, C.; Scaringe, S. A.; Lu, Y.; Rauchfuss, T. B. (2002). "An organometallic route to oligonucleotides containing phosphoroselenoate.". ChemBioChem 3 (11): 1061–1065. 
20. ^ Ravikumar, V. T.; Kumar, R. K. (2004). "Stereoselective Synthesis of Alkylphosphonates: A Facile Rearrangement of Cyanoethyl-Protected Nucleoside Phosphoramidites". Org. Proc Res. Dev. 8 (4): 603–608. doi:10.1021/op030035u. 
21. ^ "Beta-Cyanoethyl Phosphoramidites". Products.appliedbiosystems.com. https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600634. Retrieved 2009-05-12. 
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26. ^ Gryaznov, S. M.; Letsinger, R. L. (1991). "Synthesis of oligonucleotides via monomers with unprotected bases". J. Amer. Chem. Soc. 113 (15): 5876–5877. doi:10.1021/ja00015a059. 
27. ^ ^{a b} Reddy, M. P.; Hanna, N. B.; Farooqui, F. (1997). "Ultrafast Cleavage and Deprotection of Oligonucleotides Synthesis and Use of C^Ac Derivatives". Nucleosides & Nucleotides 16: 1589–1598. doi:10.1080/07328319708006236. 
28. ^ McMinn, D. (1997). "Synthesis of oligonucleotides containing 3'-alkyl amines using N-isobutyryl protected deoxyadenosine phosphoramidite". Tetrahedron Lett. 38: 3123. doi:10.1016/S0040-4039(97)00568-6. 
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35. ^ Usman, N.; Ogilvie, K. K.; Jiang, M. Y.; Cedergren, R. J. (1987). "The automated chemical synthesis of long oligoribuncleotides using 2'-O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNA". J. Amer. Chem. Soc. 109 (25): 7845–7854. doi:10.1021/ja00259a037. 
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Further reading

• Comprehensive Natural Products Chemistry, Volume 7: DNA and Aspects of Molecular Biology. Kool, Eric T.; Editor. Neth. (1999), 733 pp. Publisher: (Elsevier, Amsterdam, Neth.)
• Beaucage, S. L.; Iyer, R. P. Advances in the synthesis of oligonucleotides by the phosphoramidite approach. Tetrahedron 1992, 48, 2223–2311.
• Beaucage, S. L.; Iyer, R. P. The functionalization of oligonucleotides via phosphoramidite derivatives. Tetrahedron 1993, 49, 1925–1963.
• Beaucage, S. L.; Iyer, R. P. The synthesis of modified oligonucleotides by the phosphoramidite approach and their applications. Tetrahedron 1993, 49, 6123–6194.
• Beaucage, S L. Oligodeoxyribonucleotides synthesis. Phosphoramidite approach. Methods in Molecular Biology (Totowa, NJ, United States) (1993), 20 (Protocols for Oligonucleotides and Analogs), 33–61.
• Reese, C. B. The chemical synthesis of oligo- and poly-nucleotides: a personal commentary. Tetrahedron 2002, 58, 8893–8920.
• Brown T., Brown D. J. S. 1991. In Oligonucleotides and Analogues. A Practical Approach, ed. F Eckstein, pp.  1 – 24. Oxford: IRL

See also

• Oligonucleotide synthesis
• DNA synthesis
• Nucleic acid analogues