- Cystine tryptic agar
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Cystine tryptic agar (CTA), also known as cystine trypticase agar[1][2], is a growth medium used for the identification of microorganisms.[3]
It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose. This approach can be used to type organisms because although strains quickly gain antibiotic resistance, they rarely gain the ability to metabolize new nutrients (though exceptions are known.) For example, the following fermentation patterns have been observed:
Organism Fermentable sugars Neisseria gonorrhoeae glucose[4] Neisseria meningitidis glucose, maltose[4] Neisseria lactamica glucose, maltose, lactose[4] Neisseria mucosa glucose, maltose, sucrose[4] Moraxella catarrhalis none of the major sugars[4] Typical composition
Cystine tryptic agar typically contains (w/v)[5]:
- 2.0 % casein
- 0.05 % L-cystine
- 0.5 % sodium chloride
- 0.05% sodium sulfite
- 0.25% agar
- 0.00017% phenol red
See also
References
- ^ "BSCI424 Laboratory Media". http://www.life.umd.edu/classroom/bsci424/LabMaterialsMethods/BSCI424Media.htm. Retrieved 2008-11-17.
- ^ Hollis DG, Wiggins GL, Weaver RE (January 1969). "Neisseria lactamicus sp. n., a lactose-fermenting species resembling Neisseria meningitidis". Appl Microbiol 17 (1): 71–7. PMC 377615. PMID 4975454. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=377615.
- ^ "University of Wisconsin - Madison, Veterinary Mycology". http://www.vetmed.wisc.edu/pbs/courses/bact/labmanual/c2cta.html. Retrieved 2008-11-17.
- ^ a b c d e "Acid Detection Test- Gonorrhea - STD information from CDC". http://www.cdc.gov/std/gonorrhea/lab/tests/acid.htm.
- ^ Becton, Dickinson and Company, CTA Medium, 2005.
Growth media / agar plates Selective media AlphaproteobacteriaBrucella abortus (Brucella agar)BetaproteobacteriaDifferential media Fungal media Nonselective media Other/ungrouped media Cysteine lactose electrolyte deficient agar · Cystine tryptic agar · Endo agar · LIA slant · Müller-Hinton agar/PNP agar · R2a agar · Simmons' citrate agar · Trypticase soy agar · TSI agarCategories:- Microbiological media
- Microbiology stubs
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