Eosin methylene blue

Eosin methylene blue

Eosin methylene blue (EMB) is a selective stain for Gram-negative bacteria. It is a blend of two stains, eosin and methylene blue. A common application of this stain is in the preparation of EMB agar, a which inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between those organisms that ferment lactose versus those that do not. Organisms which ferment lactose display "nucleated colonies" -- colonies with dark centers.

On EMB if "E.coli" is grown it will give off a distinctive metallic green sheen (due to acid production). It is the only bacterium that reacts this way to EMB so it can be quickly used to identify "E. coli"

References

http://medic.med.uth.tmc.edu/path/emb.htm


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