Trypticase soy agar

Trypticase soy agar

Trypticase soy agar is a bacterial growth medium.

The medium contains enzymatic digests of casein and soybean meal which provides amino acids and other nitrogenous substances making it a nutritious medium for a variety of organisms. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH. Agar extracted from any number of organisms is used as a gelling agent.The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from mixed flora. As with any media, minor changes may be made to suit specific circumstances.

One liter of the agar contains:
*17g Tryptone
*3g Soytone - enzymatic digest of soybean meal
*2.5g Dextrose
*5g Sodium Chloride
*2.5g K2HPO4
*15g Agar

References

*http://www.madsci.org/posts/archives/2000-02/951527342.Mi.r.html


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