Gene duplication

Gene duplication
Schematic of a region of a chromosome before and after a duplication event

Gene duplication (or chromosomal duplication or gene amplification) is any duplication of a region of DNA that contains a gene; it may occur as an error in homologous recombination, a retrotransposition event, or duplication of an entire chromosome.[1] The second copy of the gene is often free from selective pressure — that is, mutations of it have no deleterious effects to its host organism. Thus it accumulates mutations faster than a functional single-copy gene, over generations of organisms.

A duplication is the opposite of a deletion. Duplications arise from an event termed unequal crossing-over that occurs during meiosis between misaligned homologous chromosomes. The chance of this happening is a function of the degree of sharing of repetitive elements between two chromosomes. The product of this recombination are a duplication at the site of the exchange and a reciprocal deletion.[2]

Contents

Gene duplication as an evolutionary event

Gene duplication is believed to play a major role in evolution; this stance has been held by members of the scientific community for over 100 years.[3] Susumu Ohno was one of the most famous developers of this theory in his classic book Evolution by gene duplication (1970).[4] Ohno argued that gene duplication is the most important evolutionary force since the emergence of the universal common ancestor.[5] Major genome duplication events are not uncommon. It is believed that the entire yeast genome underwent duplication about 100 million years ago.[6] Plants are the most prolific genome duplicators. For example, wheat is hexaploid (a kind of polyploid), meaning that it has six copies of its genome.

The duplication of a gene results in an additional copy that is free from selective pressure. One kind of view is that this allows the new copy of the gene to mutate without deleterious consequence to the organism. This freedom from consequences allows for the mutation of novel genes that could potentially increase the fitness of the organism or code for a new function. An example of this is the apparent mutation of a duplicated digestive gene in a family of ice fish into an antifreeze gene.

Another view is that both copies are equally free to accumulate degenerative mutations, so long as any defects are complemented by the other copy. This leads to a neutral "subfunctionalization" or DDC (duplication-degeneration-complementation) model,[7][8] in which the functionality of the original gene is distributed among the two copies.

The two genes that exist after a gene duplication event are called paralogs and usually code for proteins with a similar function and/or structure. By contrast, orthologous genes are ones which code for proteins with similar functions but exist in different species, and are created from a speciation event. (See Homology of sequences in genetics).

It is important (but often difficult) to differentiate between paralogs and orthologs in biological research. Experiments on human gene function can often be carried out on other species if a homolog to a human gene can be found in the genome of that species, but only if the homolog is orthologous. If they are paralogs and resulted from a gene duplication event, their functions are likely to be too different.

The paralogous segments can be repeat sequences with more than 90% sequence similarity. In such cases, they are known as low copy repeats (LCRs) though they are not highly repetitive sequences. They are mostly found in pericentronomic, subtelomeric and interstitial regions of a chromosome. The LCRs, due to their size (>1Kb), similarity, and orientation, are highly susceptible to duplications and deletions. These genomic rearrangements are caused by the mechanism of non-allelic homologous recombination. The resulting genomic variation leads to gene dosage dependent neurological disorders such as Rett-like syndrome and Pelizaeus-Merzbacher disease.[9]

Gene duplication as amplification

Gene duplication doesn't necessarily constitute a lasting change in a species' genome. In fact, such changes often don't last past the initial host organism. From the perspective of molecular genetics, amplification is one of many ways in which a gene can be overexpressed. Genetic amplification can occur artificially, as with the use of the polymerase chain reaction technique to amplify short strands of DNA in vitro using enzymes, or it can occur naturally, as described above. If it's a natural duplication, it can still take place in a somatic cell, rather than a germline cell (which would be necessary for a lasting evolutionary change).

Genomic microarrays detect Duplications

Technologies such as genomic microarrays, also called array comparative genomic hybridization (array CGH), are used to detect chromosomal abnormalities, such as microduplications, in a high throughput fashion from genomic DNA samples. In particular, DNA microarray technology can simultaneously monitor the expression levels of thousands of genes across many treatments or experimental conditions, greatly facilitating the evolutionary studies of gene regulation after gene duplication or speciation.[10][11]

Role in cancer

Duplications of oncogenes are a common cause of many types of cancer, as is the case with P70-S6 Kinase 1 amplification and breast cancer.[12] In such cases the genetic duplication occurs in a somatic cell and affects only the genome of the cancer cells themselves, not the entire organism, much less any subsequent offspring.

Common oncogene amplifications in human cancers
Cancer type Associated gene
amplifications
Prevalence of
amplification
in cancer type
(percent)
Breast cancer MYC 20[13]
ERBB2 (EGFR) 20[13]
CCND1 (Cyclin D1) 15-20[13]
FGFR1 12[13]
FGFR2 12[13]
Cervical cancer MYC 25-50[13]
ERBB2 20[13]
Colorectal cancer HRAS 30[13]
KRAS 20[13]
MYB 15-20[13]
Esophageal cancer MYC 40[13]
CCND1 25[13]
MDM2 13[13]
Gastric cancer CCNE (Cyclin E) 15[13]
KRAS 10[13]
MET 10[13]
Glioblastoma ERBB1 (EGFR) 33-50[13]
CDK4 15[13]
Head and neck cancer CCND1 50[13]
ERBB1 10[13]
MYC 7-10[13]
Hepatocellular cancer CCND1 13[13]
Neuroblastoma MYCN 20-25[13]
Ovarian cancer MYC 20-30[13]
ERBB2 15-30[13]
AKT2 12[13]
Sarcoma MDM2 10-30[13]
CDK4 10[13]
Small cell lung cancer MYC 15-20[13]

See also

References

  1. ^ Zhang J (2003). "Evolution by gene duplication: an update". Trends in Ecology & Evolution 18 (6): 292–8. doi:10.1016/S0169-5347(03)00033-8. 
  2. ^ http://www.medterms.com/script/main/art.asp?articlekey=3562
  3. ^ Taylor JS, Raes J (2004). "Duplication and divergence: the evolution of new genes and old ideas". Annu. Rev. Genet. 38: 615–43. doi:10.1146/annurev.genet.38.072902.092831. PMID 15568988. 
  4. ^ Ohno, S. (1970). Evolution by gene duplication. Springer-Verlag. ISBN 0-04-575015-7. 
  5. ^ Ohno, S. (1967). Sex Chromosomes and Sex-linked Genes. Springer-Verlag. ISBN 91-554-5776-2. 
  6. ^ Kellis M, Birren BW, Lander ES (April 2004). "Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae". Nature 428 (6983): 617–24. doi:10.1038/nature02424. PMID 15004568. 
  7. ^ Force A., Lynch M., Pickett F. B., Amores A., Yan Y. L. and Postlethwait J. (1999). "Preservation of duplicate genes by complementary, degenerative mutations.". Genetics 151 (4): 1531–1545. PMC 1460548. PMID 10101175. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1460548. 
  8. ^ Stoltzfus, A. (1999). "On the possibility of constructive neutral evolution.". J Mol Evol 49 (2): 169–181. doi:10.1007/PL00006540. PMID 10441669. 
  9. ^ Lee JA, Lupski JR (October 2006). "Genomic rearrangements and gene copy-number alterations as a cause of nervous system disorders". Neuron 52 (1): 103–21. doi:10.1016/j.neuron.2006.09.027. PMID 17015230. 
  10. ^ Mao R, Pevsner J (2005). "The use of genomic microarrays to study chromosomal abnormalities in mental retardation". Ment Retard Dev Disabil Res Rev 11 (4): 279–85. doi:10.1002/mrdd.20082. PMID 16240409. 
  11. ^ Gu X, Zhang Z, Huang W (January 2005). "Rapid evolution of expression and regulatory divergences after yeast gene duplication". Proc. Natl. Acad. Sci. U.S.A. 102 (3): 707–12. doi:10.1073/pnas.0409186102. PMC 545572. PMID 15647348. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=545572. 
  12. ^ "Entrez Gene: RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6198. 
  13. ^ a b c d e f g h i j k l m n o p q r s t u v w x y z aa ab ac Page 116 in: Kinzler, Kenneth W.; Vogelstein, Bert (2002). The genetic basis of human cancer. New York: McGraw-Hill, Medical Pub. Division. ISBN 0-07-137050-1. 

External links


Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • Gene duplication — An extra copy of a gene. Gene duplication is a key mechanism in evolution. Once a gene is duplicated, the identical genes can undergo changes and diverge to create two different genes. A duplication is the opposite of a deletion. Duplications… …   Medical dictionary

  • gene duplication — A class of DNA rearrangement that generates a supernumerary copy of a gene in the genome. This would allow each gene to evolve independently to produce distinct functions. Such a set of evolutionarily related genes can be called a gene family …   Dictionary of molecular biology

  • Duplication (Génétique) — En génétique, la duplication génique correspond à la multiplication de matériel génétique sur un chromosome. Il existe plusieurs mécanismes qui résultent à la duplication soit d´une large portion chromosomique, soit d´un gène ou bien de quelques… …   Wikipédia en Français

  • Duplication (genetique) — Duplication (génétique) En génétique, la duplication génique correspond à la multiplication de matériel génétique sur un chromosome. Il existe plusieurs mécanismes qui résultent à la duplication soit d´une large portion chromosomique, soit d´un… …   Wikipédia en Français

  • Duplication — Duplication, duplicate, and duplicator may refer to: Art and fiction Duplicate (1998 film), a Bollywood film directed by Mahesh Bhatt Duplicate (2009 film), a 2009 Malayalam film Gregory Harrison and Kim Greist The Duplicate, a children s book by …   Wikipedia

  • Duplication Interne — En génétique, la duplication interne est le fait de doubler pendant la méïose un partie d un chromosome. Ce document provient de « Duplication interne ». Catégorie : Génétique …   Wikipédia en Français

  • Duplication interne — En génétique, la duplication interne est le fait de doubler pendant la méïose une partie d un chromosome …   Wikipédia en Français

  • Duplication (génétique) — En génétique, la duplication génique correspond à la multiplication de matériel génétique sur un chromosome. Il existe plusieurs mécanismes qui résultent à la duplication soit d´une large portion chromosomique, soit d´un gène ou bien de quelques… …   Wikipédia en Français

  • Gene family — A group of genes related in structure and often in function. The genes belonging to a gene family are descended from an ancestral gene. For example, the hemoglobin genes of critical importance to red blood cells belong to one gene family created… …   Medical dictionary

  • Gene sharing — This article is about genes sharing multiple functions in an organism; for gene sharing between organisms, see horizontal gene transfer. Gene sharing is when a single polypeptide sequence, encoded by a single gene, performs multiple distinct… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”