PEP group translocation

PEP group translocation

PEP group translocation, also known as the phosphotransferase system or PTS, is a distinct method used by bacteria for sugar uptake where the source of energy is from phosphoenolpyruvate (PEP). It is known as multicomponent system that always involves enzymes of the plasma membrane and those in the cytoplasm. An example of this transport is found in E. coli cells. The system was discovered by Saul Roseman in 1964.[1]

The phosphotransferase system is involved in transporting many sugars into bacteria, including glucose, mannose, fructose and cellobiose. PTS sugars can differ between bacterial groups, mirroring the most suitable carbon sources available in the environment every group evolved. The phosphoryl group on PEP is eventually transferred to the imported sugar via several proteins. The phosphoryl group is transferred to the Enzyme E I (EI), Histidine Protein (HPr, Heat-stable Protein) and Enzyme E II (EII) to a conserved histidine residue, whereas in the Enzyme E II B (EIIB) the phosphoryl group is usually transferred to a cysteine residue and rarely to a histidine.[2]

In the process of glucose PTS transport specific of enteric bacteria, PEP transfers its phosphoryl to a histidine residue on EI. EI in turn transfers the phosphate to HPr. From HPr the phosphoryl is transferred to EIIA. EIIA is specific for glucose and it further transfers the phosphoryl group to a juxtamembrane EIIB. Finally, EIIB phosphorylates glucose as it crosses the plasma membrane through the transmembrane Enzyme II C (EIIC), forming glucose-6-phosphate.[2] The benefit of transforming glucose into glucose-6-phosphate is that it will not leak out of the cell, therefore providing a one-way concentration gradient of glucose. The HPr is common to the phosphotransferase systems of the other substrates mentioned earlier, as is the upstream EI.[3]

Proteins downstream of HPr tend to vary between the different sugars. The transfer of a phosphate group to the substrate once it has been imported through the membrane transporter prevents the transporter from recognizing the substrate again, thus maintaining a concentration gradient that favours further import of the substrate through the transporter.

With the glucose phosphotransferase system, the phosphorylation status of EIIA can have regulatory functions. For example, at low glucose concentrations phosphorylated EIIA accumulates and this activates membrane-bound adenylate cyclase. Intracellular cyclic AMP levels rise and this then activate CAP (catabolite activator protein), which is involved in the catabolite repression system, also known as glucose effect. When the glucose concentration is high, EIIA is mostly dephosphorylated and this allows it to inhibit adenylate cyclase, glycerol kinase, lactose permease, and maltose permease. Thus, as well as the PEP group translocation system being an efficient way to import substrates into the bacterium, it also links this transport to regulation of other relevant proteins.

It is an active transport. After the translocation, the metabolites transported are modified.


  1. ^ Bramley HF, Kornberg HL (July 1987). "Sequence homologies between proteins of bacterial phosphoenolpyruvate-dependent sugar phosphotransferase systems: identification of possible phosphate-carrying histidine residues". Proc. Natl. Acad. Sci. U.S.A. 84 (14): 4777–80. doi:10.1073/pnas.84.14.4777. PMC 305188. PMID 3299373. 
  2. ^ a b Lengeler, Joseph W.; Drews, Gerhard; Schlegel, Hans G. (1999). Biology of Prokaryotes. Stuttgart, Germany: Blackwell Science. pp. 83–84. ISBN 978-0-632-05357-5. 
  3. ^ Madigan, M. T., J. M. Martinko, P. V. Dunlap, and D. P. Clark. Brock biology of microorganisms. 12th ed. San Francisco, CA: Pearson/Benjamin Cummings, 2009.

External links

Wikimedia Foundation. 2010.

Игры ⚽ Нужен реферат?

Look at other dictionaries:

  • CAMP receptor protein — Protein Name=PAGENAME caption=Structure of the E. coli Cyclic AMP Receptor Protein. Symbol=CRP AltSymbols=CAP EntrezGene=289905 RefSeq=NP 289905 UniProt=P0ACK0 PDB=1I5Z cAMP receptor protein (in short CRP, also known as catabolite gene activator… …   Wikipedia

  • cAMP receptor protein — Structure of the E. coli Cyclic AMP Receptor Protein. Identifiers Symbol CRP Alt. symbols CAP …   Wikipedia

  • Mantle cell lymphoma — Classification and external resources Micrograph showing mantle cell lymphoma (bottom of image) in a biopsy of the terminal ileum. H E stain …   Wikipedia

  • AIDS — For other uses, see AIDS (disambiguation). Acquired immunodeficiency syndrome (AIDS) Classification and external resources …   Wikipedia

  • Nicotine — This article is about the chemical compound. For other uses, see Nicotine (disambiguation). Nicotine Systematic (IUPAC) name …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”