CAS number 531-75-9 YesY
PubChem 5281417
ChemSpider 4444765 YesY
Jmol-3D images Image 1
Molecular formula C15H16O9
Molar mass 340.282 g/mol
Exact mass 340.079432
 YesY (verify) (what is: YesY/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Aesculin, also rendered Æsculin or Esculin, is a glucoside that naturally occurs in the horse chestnut (Aesculus hippocastanum),[1] California Buckeye (Aesculus californica)[2] and in daphnin (the dark green resin of Daphne mezereum).


Medical uses

Aesculin is used in a microbiology laboratory to aid in the identification of bacterial species (especially Enterococci and Listeria). In fact, all strains of Group D Streptococci hydrolyze æsculin in 40% bile.

Aesculin hydrolysis test

Aesculin is incorporated into agar with ferric citrate and bile salts (bile aesculin agar).[3] Hydrolysis of the aesculin forms aesculetin (6,7-dihydroxycoumarin) and glucose. The aesculetin forms dark brown or black complexes with ferric citrate, allowing the test to be read.

The bile aesculin agar is streaked and incubated at 37°C for 24 hours. The presence of a dark brown or black halo indicates that the test is positive. A positive test can occur with Enterococcus, Aerococcus and Leuconostoc. Aesculin will fluoresce under long wave ultraviolet light (360 nm): hydrolysis of aesculin results in loss of this fluorescence.

Enterococcus will often flag positive within four hours of the agar being inoculated.

Line notes

  1. ^ Plant poisons: Aesculin]
  2. ^ C. Michael Hogan, 2008
  3. ^ National Standard Methods (UK)


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  • Aesculin — Aesculin, ein Glykosid (s.d.) von der Zusammensetzung C15H16O9 + 11/2H2O, das in der Rinde der Roßkastanie enthalten ist. Es kristallisiert mit 11/2 Molekülen Kristallwasser in seinen Nadeln, schmilzt wasserfrei gegen 205° und wird durch Säuren… …   Lexikon der gesamten Technik

  • AEsculin — [AE]s cu*lin, n. Same as {Esculin}. [1913 Webster] || …   The Collaborative International Dictionary of English

  • Aesculin —   das, s, aus der Rinde der Rosskastanie (Aesculus hippocastanum) gewonnenes Glykosid (Glucosid eines Cumarin Derivats), das als Absorptionsmittel gewisser Anteile der Sonnenstrahlung z. B. in Sonnenschutzölen enthalten ist …   Universal-Lexikon

  • Aesculin — Strukturformel Allgemeines Name Aesculin Andere Namen 6 …   Deutsch Wikipedia

  • aesculin — Esculin Es*cu lin, n. [See {Esculic}.] (Chem.) A glucoside obtained from the {[AE]sculus hippocastanum}, or horse chestnut, and characterized by its fine blue fluorescent solutions. [Written also {[ae]sculin}.] [1913 Webster] …   The Collaborative International Dictionary of English

  • aesculin — /es kyeuh lin/, n. Chem. esculin. * * * …   Universalium

  • aesculin — SYN: esculin. * * * aes·cu·lin (esґku lin) esculin …   Medical dictionary

  • aesculin — n. chemical substance …   English contemporary dictionary

  • aesculin — variant of esculin * * * /es kyeuh lin/, n. Chem. esculin. * * * aesˈcūlin noun A glucoside in horse chestnut bark • • • Main Entry: ↑Aesculus …   Useful english dictionary

  • Aesculetin — Not to be confused with aesculin or aescin. Aesculetin[1] IUPAC name …   Wikipedia

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