- Nucleic acid methods
-
For protein methods, see Protein methods.
Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
- Purification
- Phenol-chloroform extraction
- minicolumn purification
- RNA extraction
- Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: Q-PCR
- high-throughput relative abundance DNA microarray
- high-throughput absolute abundance SAGE
- Size: Gel electrophoresis
- Synthesis
- De novo: Oligonucleotide synthesis
- Amplification: PCR
- Kinetics
- Other
- Nucleic acid simulations
- DNA sequencing
- Bisulfite sequencing
- Expression cloning
- Southern blot
- Northern blot
- Sucrose gradient centrifugation
- Radioactivity in biological research
- Lab-on-a-chip
- Nuclear run-on assay
- Fluorescent in situ hybridization
- several Bioinformatics methods, such as RNA structure prediction
See also
References
- ^ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ^ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques 19 (3): 151–158. PMC 2563918. PMID 19137101. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2563918.
External links
Molecular biology Overview Element(Genetic • Heredity)
Promoter (Pribnow box, TATA box) • Operon (gal operon, lac operon, trp operon) • Intron • Exon • Terminator • Enhancer • Repressor (lac repressor, trp repressor) • Silencer • Histone methylationLinked LifeEngineering Conceptmitosis • cell signalling • Post-transcriptional modification and Post-translational modification • Dry Lab/Wet labTechniqueCell culture • model organisms (such as C57BL/6 mice) • method (Nucleic acid • Protein) • Fluorescence, Pigment & Radioactivity
- High-throughput Technique (-omics): DNA microarray • Mass spectrometry • Lab-on-a-chip
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