RNA extraction

RNA extraction

RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. [cite journal |author=Peirson SN, Butler JN |title=RNA extraction from mammalian tissues |journal=Methods Mol. Biol. |volume=362 |issue= |pages=315–27 |year=2007 |pmid=17417019 |doi=10.1007/978-1-59745-257-1_22] Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction. [cite journal |author=Chomczynski P, Sacchi N |title=The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on |journal=Nat Protoc |volume=1 |issue=2 |pages=581–5 |year=2006 |pmid=17406285 |doi=10.1038/nprot.2006.83] [cite journal |author=Bird IM |title=Extraction of RNA from cells and tissue |journal=Methods Mol. Med. |volume=108 |issue= |pages=139–48 |year=2005 |pmid=16028681] This method often uses a proprietary formulation of this reagent called Trizol.

ee also

*RNA quality control see the following page http://RNA-integrity.gene-quantification.info/
*DNA extraction
*Phenol-chloroform extraction
*Ethanol precipitation
*Column purification

References

External links

* [http://www.protocol-online.org/prot/Molecular_Biology/RNA/RNA_Extraction/ RNA extraction] Protocol On-line


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