Nuclear run-on

A nuclear run-on assay is conducted to identify the genes that are being transcribed at a certain time. Cell nuclei are isolated rapidly, and incubated with labelled nucleotides and the results are hybridized to a slot blot, which is then exposed to film. It was originally developed by Gariglio et al. (1981) and Brown et al. (1984) (see discussion). Adding actinomycin-D to the reaction buffer is sufficient to stop transcription, while other toxins, such as α-amanitin, show different effects, making it a good way to assay the effects of these compounds.

This method allows changes in transcription rates to be measured, which often differ from steady-state mRNA levels used in microarrays. Although the method is time-consuming and requires the use of radioactivity and large numbers of cells, it remains the most reliable method to measure transcription rates directly. Several attempts recently have been made to make this method microarray-compatible, but other methods are much more favorable. To have a publication quality blot about 10⁷ nuclei are needed, but 10⁶ nuclei are sufficient to give results: these quantities of cells are higher than for most protocols.

It is often cited that this method has been surpassed by microarrays, even though this method compares transcription rates and not total transcript expression levels, which differ.

It is incompatible with microarray experiments due to the fact eukaryotic polymerases are more intolerant towards fluorescently labeled or aminoallyl nucleotides, so UTP with phosphorus-32, or phosphorus-33, on the alpha phosphate group is normally used. Cheadle et al. (2005) used spotted nylon blots to obtain a high-throughput analysis of T-cell activation. Garcia-Martinez et al (2004)developed a protocol for the yeast S. cerevisiae (Genomic run-on, GRO) that allows for the calculation of transcription rates (TRs) for all yeast genes. Those authors also used the calculated TRs and the steady-state mRNA amounts of all genes to determine, at global scale, the mRNA stabilities for all yeast mRNAs.

Alternative microarray methods have recently been developed, mainly PolII RIP-chip: RNA immunoprecipitation of RNA polymerase II with phosphorylated C-terminal domain directed antibodies and hybridization on a microarray slide or chip (the word chip in the name stems from "ChIP-chip" where a special affymetrix genechip was required). A comparison of methods based on run-on and ChIP-chip has been made in yeast (Pelechano et al., 2009). A general correspondence of both methods has been detected but GRO is more sensitive and quantitative. It has to be considered that run-on only detects elongating RNA polymerases whereas ChIP-chip detects all present RNA polymerases, including backtracked ones.

The main issue on the validity of run on assay results is the presence of artifacts due to the isolation step: some transcripts may stop prematurely others might start out of place.

Another point to add is about the nuclei: There are various methods that require nuclear isolation. The problem is that the nuclear membrane is contiguous with the ER which is rich in protein and cytoplasmic mRNA; some methods require pure nuclear lysate while others, like a nuclear run-on, require "dirty" nuclei with an intact nuclear membrane even if the ER is attached. Some types of cell are notoriously difficult to isolate this way such as lymphocytes, which require a sucrose gradient purification. Yeast cells do not require nuclei isolation. Permeabilized cells (with sarkosil) can be used. This is a clear technical advantage.

See also

• Radioactivity in biology

References

Protocol [1]

http://www.mblab.gla.ac.uk/~julian/dict2.cgi?4568 Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability. Cheadle, C et al. 2005, BMC Genomics 6:75, pp. 75-91.

Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms. García-Martínez J, Aranda A, Pérez-Ortín JE. Mol Cell. 2004 Jul 23;15(2):303-13.PMID: 15260981.

Regulon-specific control of transcription elongation across the yeast genome. Pelechano V, Jimeno-González S, Rodríguez-Gil A, García-Martínez J, Pérez-Ortín JE, Chávez S. PLoS Genet. 2009 Aug;5(8):e1000614. Epub 2009 Aug 21.PMID: 19696888