DDB2

Damage-specific DNA binding protein 2, 48kDa
Rendering based on PDB 3EI4.
Available structures PDB 3EI4, 3I7L
Identifiers
Symbols	DDB2; DDBB; FLJ34321; UV-DDB2
External IDs	OMIM: 600811 MGI: 1355314 HomoloGene: 83 GeneCards: DDB2 Gene
Gene Ontology Molecular function • DNA binding • damaged DNA binding • ubiquitin-protein ligase activity • protein binding Cellular component • nucleus • nucleoplasm • nucleoplasm • protein complex Biological process • protein polyubiquitination • nucleotide-excision repair, DNA damage removal • DNA repair • nucleotide-excision repair • pyrimidine dimer repair • response to UV • protein autoubiquitination Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1643	107986
Ensembl	ENSG00000134574	ENSMUSG00000002109
UniProt	Q92466	Q99J79
RefSeq (mRNA)	NM_000107.2	NM_028119.5
RefSeq (protein)	NP_000098.1	NP_082395.2
Location (UCSC)	Chr 11: 47.24 – 47.26 Mb	Chr 2: 91.05 – 91.08 Mb
PubMed search	[1]	[2]

DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene.^[1][2]

DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.^[3]

Interactions

DDB2 has been shown to interact with CUL4A,^[4] CUL4B^[4] and DDB1.^[5][6][4]

External Links

• GeneReviews/NIH/NCBI/UW entry on Xeroderma Pigmentosum

References

1. ^ Keeney S, Chang GJ, Linn S (Nov 1993). "Characterization of a human DNA damage binding protein implicated in xeroderma pigmentosum E". J Biol Chem 268 (28): 21293–300. PMID 8407967. 
2. ^ Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S (Feb 1996). "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein". Genomics 29 (1): 62–9. doi:10.1006/geno.1995.1215. PMID 8530102. 
3. ^ "Entrez Gene: DDB2 damage-specific DNA binding protein 2, 48kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1643. 
4. ^ ^{a b c} Guerrero-Santoro, Jennifer; Kapetanaki Maria G, Hsieh Ching L, Gorbachinsky Ilya, Levine Arthur S, Rapić-Otrin Vesna (Jul. 2008). "The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A". Cancer Res. (United States) 68 (13): 5014–22. doi:10.1158/0008-5472.CAN-07-6162. PMID 18593899. 
5. ^ Bergametti, Françoise; Sitterlin Delphine, Transy Catherine (Jul. 2002). "Turnover of hepatitis B virus X protein is regulated by damaged DNA-binding complex". J. Virol. (United States) 76 (13): 6495–501. doi:10.1128/JVI.76.13.6495-6501.2002. ISSN 0022-538X. PMC 136256. PMID 12050362. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=136256. 
6. ^ Martinez, E; Palhan V B, Tjernberg A, Lymar E S, Gamper A M, Kundu T K, Chait B T, Roeder R G (Oct. 2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo". Mol. Cell. Biol. (United States) 21 (20): 6782–95. doi:10.1128/MCB.21.20.6782-6795.2001. ISSN 0270-7306. PMC 99856. PMID 11564863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99856. 

Further reading

• El-Deiry WS (2003). "Transactivation of repair genes by BRCA1.". Cancer Biol. Ther. 1 (5): 490–1. PMID 12496474. 
• Qian YW, Wang YC, Hollingsworth RE, et al. (1993). "A retinoblastoma-binding protein related to a negative regulator of Ras in yeast.". Nature 364 (6438): 648–52. doi:10.1038/364648a0. PMID 8350924. 
• Taunton J, Hassig CA, Schreiber SL (1996). "A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.". Science 272 (5260): 408–11. doi:10.1126/science.272.5260.408. PMID 8602529. 
• Nichols AF, Ong P, Linn S (1996). "Mutations specific to the xeroderma pigmentosum group E Ddb- phenotype.". J. Biol. Chem. 271 (40): 24317–20. doi:10.1074/jbc.271.40.24317. PMID 8798680. 
• Hayes S, Shiyanov P, Chen X, Raychaudhuri P (1998). "DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1.". Mol. Cell. Biol. 18 (1): 240–9. PMC 121483. PMID 9418871. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=121483. 
• Martinez E, Palhan VB, Tjernberg A, et al. (2001). "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo.". Mol. Cell. Biol. 21 (20): 6782–95. doi:10.1128/MCB.21.20.6782-6795.2001. PMC 99856. PMID 11564863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99856. 
• Chen X, Zhang Y, Douglas L, Zhou P (2002). "UV-damaged DNA-binding proteins are targets of CUL-4A-mediated ubiquitination and degradation.". J. Biol. Chem. 276 (51): 48175–82. doi:10.1074/jbc.M106808200. PMID 11673459. 
• Sun NK, Kamarajan P, Huang H, Chao CC (2002). "Restoration of UV sensitivity in UV-resistant HeLa cells by antisense-mediated depletion of damaged DNA-binding protein 2 (DDB2).". FEBS Lett. 512 (1-3): 168–72. doi:10.1016/S0014-5793(02)02250-0. PMID 11852074. 
• Tan T, Chu G (2002). "p53 Binds and activates the xeroderma pigmentosum DDB2 gene in humans but not mice.". Mol. Cell. Biol. 22 (10): 3247–54. doi:10.1128/MCB.22.10.3247-3254.2002. PMC 133779. PMID 11971958. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133779. 
• Rapić-Otrin V, McLenigan MP, Bisi DC, et al. (2002). "Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation.". Nucleic Acids Res. 30 (11): 2588–98. doi:10.1093/nar/30.11.2588. PMC 117178. PMID 12034848. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=117178. 
• Bergametti F, Sitterlin D, Transy C (2002). "Turnover of hepatitis B virus X protein is regulated by damaged DNA-binding complex.". J. Virol. 76 (13): 6495–501. doi:10.1128/JVI.76.13.6495-6501.2002. PMC 136256. PMID 12050362. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=136256. 
• Bontron S, Lin-Marq N, Strubin M (2002). "Hepatitis B virus X protein associated with UV-DDB1 induces cell death in the nucleus and is functionally antagonized by UV-DDB2.". J. Biol. Chem. 277 (41): 38847–54. doi:10.1074/jbc.M205722200. PMID 12151405. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Sun NK, Lu HP, Chao CC (2003). "Overexpression of damaged-DNA-binding protein 2 (DDB2) potentiates UV resistance in hamster V79 cells.". Chang Gung medical journal 25 (11): 723–33. PMID 12553360. 
• Groisman R, Polanowska J, Kuraoka I, et al. (2003). "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage.". Cell 113 (3): 357–67. doi:10.1016/S0092-8674(03)00316-7. PMID 12732143. 
• Rapić-Otrin V, Navazza V, Nardo T, et al. (2004). "True XP group E patients have a defective UV-damaged DNA binding protein complex and mutations in DDB2 which reveal the functional domains of its p48 product.". Hum. Mol. Genet. 12 (13): 1507–22. doi:10.1093/hmg/ddg174. PMID 12812979. 
• Fitch ME, Nakajima S, Yasui A, Ford JM (2004). "In vivo recruitment of XPC to UV-induced cyclobutane pyrimidine dimers by the DDB2 gene product.". J. Biol. Chem. 278 (47): 46906–10. doi:10.1074/jbc.M307254200. PMID 12944386.