ORC2L

Origin recognition complex, subunit 2
Identifiers
Symbols	ORC2; ORC2
External IDs	OMIM: 601182 MGI: 1328306 HomoloGene: 4512 GeneCards: ORC2 Gene
Gene Ontology Molecular function • DNA replication origin binding • protein binding Cellular component • heterochromatin • origin recognition complex • condensed chromosome inner kinetochore • nucleus • nucleoplasm • nucleoplasm • nuclear origin of replication recognition complex Biological process • cell cycle checkpoint • G1/S transition of mitotic cell cycle • S phase of mitotic cell cycle • negative regulation of transcription from RNA polymerase II promoter • M/G1 transition of mitotic cell cycle • mitotic cell cycle • DNA replication • DNA-dependent DNA replication initiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4999	18393
Ensembl	ENSG00000115942	ENSMUSG00000026037
UniProt	Q13416	Q543F8
RefSeq (mRNA)	NM_006190.4	NM_001025378
RefSeq (protein)	NP_006181	NP_001020549
Location (UCSC)	Chr 2: 201.77 – 201.83 Mb	Chr 1: 58.52 – 58.56 Mb
PubMed search	[1]	[2]

Origin recognition complex subunit 2 is a protein that in humans is encoded by the ORC2L gene.^[1][2]

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.^[2]

Interactions

ORC2L has been shown to interact with ORC1L,^[3][4][5][6] Replication protein A1,^[3] ORC4L,^[3][7][8][5] ORC5L,^[3][9][7][5] ORC3L,^{[3][7][10][11][5]} MCM4,^[3] CDC6,^[3][12] MCM5,^[3] MCM6,^[3] MCM7,^[3] MCM10,^[13] DBF4,^[3] MCM2^[3] and ORC6L.^[3][5]

References

1. ^ Takahara K, Bong M, Brevard R, Eddy RL, Haley LL, Sait SJ, Shows TB, Hoffman GG, Greenspan DS (Oct 1996). "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L". Genomics 31 (1): 119–122. doi:10.1006/geno.1996.0018. PMID 8808289. 
2. ^ ^{a b} "Entrez Gene: ORC2L origin recognition complex, subunit 2-like (yeast)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4999. 
3. ^ ^{a b c d e f g h i j k l m} Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (Mar. 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. (England) 327 (1): 111–128. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
4. ^ Fujita, Masatoshi; Ishimi Yukio, Nakamura Hiromu, Kiyono Tohru, Tsurumi Tatsuya (Mar. 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. (United States) 277 (12): 10354–10361. doi:10.1074/jbc.M111398200. ISSN 0021-9258. PMID 11779870. 
5. ^ ^{a b c d e} Vashee, S; Simancek P, Challberg M D, Kelly T J (Jul. 2001). "Assembly of the human origin recognition complex". J. Biol. Chem. (United States) 276 (28): 26666–26673. doi:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433. 
6. ^ Méndez, Juan; Zou-Yang X Helena, Kim So-Young, Hidaka Masumi, Tansey William P, Stillman Bruce (Mar. 2002). "Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication". Mol. Cell (United States) 9 (3): 481–491. doi:10.1016/S1097-2765(02)00467-7. ISSN 1097-2765. PMID 11931757. 
7. ^ ^{a b c} Dhar, S K; Delmolino L, Dutta A (Aug. 2001). "Architecture of the human origin recognition complex". J. Biol. Chem. (United States) 276 (31): 29067–29071. doi:10.1074/jbc.M103078200. ISSN 0021-9258. PMID 11395502. 
8. ^ Quintana, D G; Hou Zh, Thome K C, Hendricks M, Saha P, Dutta A (Nov. 1997). "Identification of HsORC4, a member of the human origin of replication recognition complex". J. Biol. Chem. (UNITED STATES) 272 (45): 28247–28251. doi:10.1074/jbc.272.45.28247. ISSN 0021-9258. PMID 9353276. 
9. ^ Quintana, D G; Thome K C, Hou Z H, Ligon A H, Morton C C, Dutta A (Oct. 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". J. Biol. Chem. (UNITED STATES) 273 (42): 27137–27145. doi:10.1074/jbc.273.42.27137. ISSN 0021-9258. PMID 9765232. 
10. ^ Matsuoka, Shuhei; Ballif Bryan A, Smogorzewska Agata, McDonald E Robert, Hurov Kristen E, Luo Ji, Bakalarski Corey E, Zhao Zhenming, Solimini Nicole, Lerenthal Yaniv, Shiloh Yosef, Gygi Steven P, Elledge Stephen J (May. 2007). "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage". Science (United States) 316 (5828): 1160–1166. doi:10.1126/science.1140321. PMID 17525332. 
11. ^ Pinto, S; Quintana D G, Smith P, Mihalek R M, Hou Z H, Boynton S, Jones C J, Hendricks M, Velinzon K, Wohlschlegel J A, Austin R J, Lane W S, Tully T, Dutta A (May. 1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant". Neuron (UNITED STATES) 23 (1): 45–54. doi:10.1016/S0896-6273(00)80752-7. ISSN 0896-6273. PMID 10402192. 
12. ^ Méndez, J; Stillman B (Nov. 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. (UNITED STATES) 20 (22): 8602–8612. doi:10.1128/MCB.20.22.8602-8612.2000. ISSN 0270-7306. PMC 102165. PMID 11046155. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=102165. 
13. ^ Izumi, M; Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon K Y, Hurwitz J, Yatagai F, Hanaoka F (Dec. 2000). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase". Nucleic Acids Res. (ENGLAND) 28 (23): 4769–4777. doi:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=115166. 

Further reading

• Gavin KA, Hidaka M, Stillman B (1996). "Conserved initiator proteins in eukaryotes". Science 270 (5242): 1667–1671. doi:10.1126/science.270.5242.1667. PMID 7502077. 
• Quintana DG, Hou Zh, Thome KC et al. (1997). "Identification of HsORC4, a member of the human origin of replication recognition complex". J. Biol. Chem. 272 (45): 28247–28251. doi:10.1074/jbc.272.45.28247. PMID 9353276. 
• Saha P, Thome KC, Yamaguchi R et al. (1998). "The human homolog of Saccharomyces cerevisiae CDC45". J. Biol. Chem. 273 (29): 18205–18209. doi:10.1074/jbc.273.29.18205. PMID 9660782. 
• Ritzi M, Baack M, Musahl C et al. (1998). "Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin". J. Biol. Chem. 273 (38): 24543–24549. doi:10.1074/jbc.273.38.24543. PMID 9733749. 
• Quintana DG, Thome KC, Hou ZH et al. (1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". J. Biol. Chem. 273 (42): 27137–27145. doi:10.1074/jbc.273.42.27137. PMID 9765232. 
• Pinto S, Quintana DG, Smith P et al. (1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant". Neuron 23 (1): 45–54. doi:10.1016/S0896-6273(00)80752-7. PMID 10402192. 
• Jiang W, McDonald D, Hope TJ, Hunter T (1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". EMBO J. 18 (20): 5703–5713. doi:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1171637. 
• Natale DA, Li CJ, Sun WH, DePamphilis ML (2000). "Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals". EMBO J. 19 (11): 2728–2738. doi:10.1093/emboj/19.11.2728. PMC 212765. PMID 10835370. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=212765. 
• Thome KC, Dhar SK, Quintana DG et al. (2001). "Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins". J. Biol. Chem. 275 (45): 35233–35241. doi:10.1074/jbc.M005765200. PMID 10954718. 
• Izumi M, Yanagi K, Mizuno T et al. (2001). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase". Nucleic Acids Res. 28 (23): 4769–4777. doi:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=115166. 
• Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). "Assembly of the human origin recognition complex". J. Biol. Chem. 276 (28): 26666–26673. doi:10.1074/jbc.M102493200. PMID 11323433. 
• Dhar SK, Delmolino L, Dutta A (2001). "Architecture of the human origin recognition complex". J. Biol. Chem. 276 (31): 29067–29071. doi:10.1074/jbc.M103078200. PMID 11395502. 
• Dhar SK, Yoshida K, Machida Y et al. (2001). "Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin". Cell 106 (3): 287–296. doi:10.1016/S0092-8674(01)00458-5. PMID 11509178. 
• Izumi M, Yatagai F, Hanaoka F (2002). "Cell cycle-dependent proteolysis and phosphorylation of human Mcm10". J. Biol. Chem. 276 (51): 48526–31. doi:10.1074/jbc.M107190200. PMID 11602595. 
• Fujita M, Ishimi Y, Nakamura H et al. (2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. 277 (12): 10354–10361. doi:10.1074/jbc.M111398200. PMID 11779870. 
• Schaarschmidt D, Ladenburger EM, Keller C, Knippers R (2002). "Human Mcm proteins at a replication origin during the G1 to S phase transition". Nucleic Acids Res. 30 (19): 4176–4185. doi:10.1093/nar/gkf532. PMC 140533. PMID 12364596. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=140533. 
• Matheos D, Ruiz MT, Price GB, Zannis-Hadjopoulos M (2002). "Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication". Biochim. Biophys. Acta 1578 (1–3): 59–72. doi:10.1016/S0167-4781(02)00497-9. PMID 12393188. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–128. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.