MCM10

Minichromosome maintenance complex component 10
Identifiers
Symbols	MCM10; CNA43; DNA43; MGC126776
External IDs	OMIM: 609357 MGI: 1917274 HomoloGene: 41275 GeneCards: MCM10 Gene
Gene Ontology Molecular function • protein binding • metal ion binding Cellular component • nucleus • nucleoplasm • nucleoplasm Biological process • cell cycle checkpoint • G1/S transition of mitotic cell cycle • M/G1 transition of mitotic cell cycle • mitotic cell cycle • DNA replication Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	55388	70024
Ensembl	ENSG00000065328	ENSMUSG00000026669
UniProt	Q7L590	n/a
RefSeq (mRNA)	NM_018518.4	NM_027290.3
RefSeq (protein)	NP_060988.3	NP_081566.2
Location (UCSC)	Chr 10: 13.2 – 13.25 Mb	Chr 2: 4.91 – 4.93 Mb
PubMed search	[1]	[2]

Protein MCM10 homolog is a protein that in humans is encoded by the MCM10 gene.^[1][2][3]

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.^[3]

Interactions

MCM10 has been shown to interact with ORC2L.^[1]

References

1. ^ ^{a b} Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F (Dec 2000). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G2 phase". Nucleic Acids Res 28 (23): 4769–77. doi:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=115166. 
2. ^ Chattopadhyay S, Bielinsky AK (Sep 2007). "Human Mcm10 Regulates the Catalytic Subunit of DNA Polymerase-α and Prevents DNA Damage during Replication". Mol Biol Cell 18 (10): 4085–95. doi:10.1091/mbc.E06-12-1148. PMC 1995709. PMID 17699597. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1995709. 
3. ^ ^{a b} "Entrez Gene: MCM10 MCM10 minichromosome maintenance deficient 10 (S. cerevisiae)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=55388. 

Further reading

• Morimoto S (2002). "[Abnormal myocardial contractile regulation mechanism in familial hypertrophic and dilated cardiomyopathies--functional analysis of molecular mutant troponin in in-vitro]". Fukuoka Igaku Zasshi 93 (1): 1–5. PMID 11889827. 
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072. 
• Izumi M, Yatagai F, Hanaoka F (2002). "Cell cycle-dependent proteolysis and phosphorylation of human Mcm10". J. Biol. Chem. 276 (51): 48526–31. doi:10.1074/jbc.M107190200. PMID 11602595. 
• Wohlschlegel JA, Dhar SK, Prokhorova TA, et al. (2002). "Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45". Mol. Cell 9 (2): 233–40. doi:10.1016/S1097-2765(02)00456-2. PMID 11864598. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Christensen TW, Tye BK (2004). "Drosophila Mcm10 Interacts with Members of the Prereplication Complex and Is Required for Proper Chromosome Condensation". Mol. Biol. Cell 14 (6): 2206–15. doi:10.1091/mbc.E02-11-0706. PMC 194871. PMID 12808023. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=194871. 
• Cook CR, Kung G, Peterson FC, et al. (2003). "A novel zinc finger is required for Mcm10 homocomplex assembly". J. Biol. Chem. 278 (38): 36051–8. doi:10.1074/jbc.M306049200. PMID 12844493. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Izumi M, Yatagai F, Hanaoka F (2004). "Localization of human Mcm10 is spatially and temporally regulated during the S phase". J. Biol. Chem. 279 (31): 32569–77. doi:10.1074/jbc.M314017200. PMID 15136575. 
• Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature 429 (6990): 375–81. doi:10.1038/nature02462. PMID 15164054. 
• Yoshida K, Inoue I (2004). "Expression of MCM10 and TopBP1 is regulated by cell proliferation and UV irradiation via the E2F transcription factor". Oncogene 23 (37): 6250–60. doi:10.1038/sj.onc.1207829. PMID 15195143. 
• Ramachandran N, Hainsworth E, Bhullar B, et al. (2004). "Self-assembling protein microarrays". Science 305 (5680): 86–90. doi:10.1126/science.1097639. PMID 15232106. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
• Zhu W, Ukomadu C, Jha S, et al. (2007). "Mcm10 and And-1/CTF4 recruit DNA polymerase α to chromatin for initiation of DNA replication". Genes Dev. 21 (18): 2288–99. doi:10.1101/gad.1585607. PMC 1973143. PMID 17761813. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1973143. 
• Robertson, P. D., Warren, E. M., Zhang, H., et al. (2007). "DOMAIN ARCHITECTURE AND BIOCHEMICAL CHARACTERIZATION OF VERTEBRATE MCM10". J Biol Chem 283 (6): 3338–48. doi:10.1074/jbc.M706267200. PMC 2753450. PMID 18065420. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2753450. 
• Warren, E. M., Vaithiyalingam, S.R., Haworth, J., Greer, B., Bielinsky, A.K., Chazin, W.J., and Eichman, B.F. (2008). "Structural Basis for DNA Binding by Replication Initiator Mcm10". Structure 16 (12): 1892–1901. doi:10.1016/j.str.2008.10.005. PMC 2636851. PMID 19081065. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2636851.