Nitric oxide dioxygenase

Nitric oxide dioxygenase
nitric oxide dioxygenase
PDB 1gvh.jpg
E. coli flavohemoglobin/NOD structure. green = reductase domain, blue = hemoglobin domain.[1]
Identifiers
EC number 1.14.12.17
CAS number 214466-78-1
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Nitric oxide dioxygenase (EC 1.14.12.17) is an enzyme that catalyzes the conversion of toxic nitric oxide (NO) to nitrate (NO−
3). The net reaction for the reaction catalyzed by nitric oxide dioxygenase is shown below:

  • 2NO + 2O2 + NAD(P)H → 2NO3- + NAD(P)+ + H+

Nitric oxide is a ubiquitous small molecule that is integrated in a wide variety of physiological processes including smooth muscle vasodilation, platelet disaggregation, neurotransmission, and immune response to bacterial infection.[2][3] Overproduction of this signaling molecule can be lethal to cells by poisoning cellular energy production. The most sensitive targets of NO are aconitase, an enzyme that catalyzes the isomerization of citrate to isocitrate in the citric acid cycle, and cytochrome oxidase, the last enzyme in the respiratory electron transport chain of mitochondria.[4] Additionally NO, with its lone radical on the nitrogen atom, is implicated in a number of secondary mechanisms of toxicity, including catalase inhibition (resulting in hydrogen peroxide toxicity), Fe-S center iron liberation, and the formation of dinitosyl-iron complexes.

Due to the potential lethality of NO, cells benefitted greatly from the evolution of an enzyme capable of catalyzing the conversion of toxic NO to nitrate. A 'nitric oxide dioxygenase' is an enzyme that is capable of carrying out this reaction. NO dioxygenase belongs to the family of oxidoreductases, more specifically those acting on paired donors, with O2 as oxidant and with incorporation of two atoms of oxygen into the other donor.

Contents

Reaction mechanism

The mechanism of action has still not been entirely deduced, however, the leading theory suggests that the conversion is carried out through a series of redox reactions involving iron centers as shown in the series of half reactions below:[5]

Step Reaction
FAD reduction NAD(P)H + FAD + H+ → NAD(P)+ + FADH2
Iron reduction 1 FADH2 + Fe3+ → Fe2+ + FADH + H+
Iron Reduction 2 FADH + Fe3+ → FAD + Fe2+ + H+
O2 Binding Fe2+ + O2 → Fe3+(O2-)
NO dioxygenation Fe3+(O2-) + NO → Fe3+ + NO3-

Another theory developed more recently (2009) suggests that a NO dioxygenase activity could also proceed through phenolic nitration via a putative heme-peroxynitrite intermediate.[6]

The most well studied NO dioxygenase is flavohemoglobin (flavoHb), shown to the right: Studies have shown that flavohemoglobins are induced by NO, nitrite, nitrate, and NO-releasing agents in various bacteria and fungi.[5][7] Additionally, flavoHbs have been shown to protect bacteria, yeast, and Dictyostelium discoideum against growth inhibition and damage mediated via NO.[7][8][9]

Discovery

Nitric oxide dioxygenase was discovered, and first reported in 1998, as an inducible O2-dependent enzymatic activity that protected bacteria against nitric oxide toxicity.[10] The enzyme was identified with the E. coli flavohemoglobin.[11]

More recently, another protein has been identified as a NO dioxygenase - rhodobacter sphaeroides haem protein (SHP), a novel cytochrome with NO dioxygenase activity.[12][13] Although the biological function of SHP has yet to be identified, SHP has been shown, that with oxygen bound, it can react rapidly with nitric oxide to form nitrate.[12]

Structure and molecular function

The flavohemoglobin protein contains two domains: an oxidoreductase FAD-binding domain, and a b-type heme-containing "globin" domain and optionally an oxidoreductase NAD-binding domain. The reductase domain supplies an electron to the heme iron to achieve a high rate of catalytic NO dioxygenation. In addition to numerous flavohemoglobins, many distantly related members of the hemoglobin superfamily including the muscle myoglobin, the non-symbiotic plant hemoglobin and symbiotic plant leghemoglobin, the neuronal neuroglobin, and the mammalian cytoplasmic cytoglobin[14][15] appear to function as nitric oxide dioxygenases (NODs), although the cellular electron donor(s) for many globins have yet to be defined. Electron donors may include ascorbate, cytochrome b5 or ferredoxin reductase.[16] The catalytic NO dioxygenation can be written in its simplest form:

NO + O2 + e- \rightleftharpoons NO3-

Catalysis is very efficient. The reported bimolecular NO dioxygenation rate constants range from 2 x 107 M−1s−1 for cytoglobin to 3 x 109 M−1s−1 for flavohemoglobin, and turnover rates range from 1 to 700 s−1. Structure, O2 binding, and reduction of globins appear optimized for a NO dioxygenase function.

Physiological function

Historically, nitric oxide dioxygenase (around 1.8 billion years ago) served to provide the modern day analogue of hemoglobin/myoglobin function for oxygen storage and transport. Gardner et al. (1998) suggested that the first hemoglobin/myoglobin probably functioned as an enzyme utilizing bound ‘activated’ oxygen gas to dioxygenate NO in microbes.[17]

The wide diversity of multicellular organisms benefitting from the oxygen storage and transport functions of myoglobin/hemoglobin appeared much later (approximately 0.5 billion years ago).


NODs are now known to serve two important physiological functions in diverse life forms. They prevent NO toxicity and regulate NO signalling. NODs belong to the larger family of well-established free radical and reactive oxygen detoxifying enzymes that includes superoxide dismutase, catalase, and peroxidase.

Distribution in nature

NODs, as well as many hemoglobins that function as NODs, are distributed to most life forms including bacteria, fungi, protists, worms, plants and animals. In fact, nitric oxide dioxygenation appears to be a primal function for members of the hemoglobin superfamily. Moreover, it is becoming increasingly evident that the NOD function of globins is much more common than the paradigmatic O2 transport-storage function of red cell hemoglobin[18] which was first investigated and reported over a century earlier by Felix Hoppe-Seyler and others.[19] Other proteins that may act as NODs include mammalian microsomal cytochrome P450(s)[20] and a novel O2-binding cytochrome b from Rhodobacter sphaeroides.[12]

Technologies

Inhibitors of the NODs are being developed for application as microbial antibiotics,[21][22] anti-tumor agents and modulators of NO signalling. The most prominent class of inhibitor of NO dioxygenase to date is imidazole antibiotics. Imidazoles have been shown to coordinate with the heme iron atom of microbial flavohemoglobin, impair ferric heme reduction, produce uncompetitive inhibition with respect to O2 and NO, and inhibit NO metabolism by yeasts and bacteria.[23] Specifically, imidazoles bearing bulky aromatic substituents have been shown to have potential for selective and high-affinity inhibition of NO dioxygenase function by coordinating the catalytic heme iron and "fitting" within the large hydrophobic distal heme pocket.[23][24][25] As a result, imidazole engineering has been suggested as a means to specifically inhibit NO dioxygenases.

In addition, genetically modified plants with heterologous flavohemoglobin-NODs are being developed to limit NO toxicity created by metabolism of nitrogen fertilizers by soil microbes and as a means towards plant self-fertilization through absorption of environmental NO.

References

  1. ^ PDB 1gvh; Ilari A, Bonamore A, Farina A, Johnson KA, Boffi A (June 2002). "The X-ray structure of ferric Escherichia coli flavohemoglobin reveals an unexpected geometry of the distal heme pocket". J. Biol. Chem. 277 (26): 23725–32. doi:10.1074/jbc.M202228200. PMID 11964402. 
  2. ^ Moncada S, Palmer RM, Higgs EA (June 1991). "Nitric oxide: physiology, pathophysiology, and pharmacology". Pharmacol. Rev. 43 (2): 109–42. PMID 1852778. 
  3. ^ Fang FC (October 2004). "Antimicrobial reactive oxygen and nitrogen species: concepts and controversies". Nat. Rev. Microbiol. 2 (10): 820–32. doi:10.1038/nrmicro1004. PMID 15378046. 
  4. ^ Gardner PR, Costantino G, Szabó C, Salzman AL (October 1997). "Nitric oxide sensitivity of the aconitases". J. Biol. Chem. 272 (40): 25071–6. doi:10.1074/jbc.272.40.25071. PMID 9312115. 
  5. ^ a b Gardner PR, Gardner AM, Martin LA, Salzman AL (September 1998). "Nitric oxide dioxygenase: an enzymic function for flavohemoglobin". Proc. Natl. Acad. Sci. U.S.A. 95 (18): 10378–83. doi:10.1073/pnas.95.18.10378. PMC 27902. PMID 9724711. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27902. 
  6. ^ Schopfer MP, Mondal B, Lee DH, Sarjeant AA, Karlin KD (August 2009). "Heme/O2/*NO nitric oxide dioxygenase (NOD) reactivity: phenolic nitration via a putative heme-peroxynitrite intermediate". J. Am. Chem. Soc. 131 (32): 11304–5. doi:10.1021/ja904832j. PMC 2747244. PMID 19627146. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2747244. 
  7. ^ a b Gardner PR, Gardner AM, Martin LA, Dou Y, Li T, Olson JS, Zhu H, Riggs AF (October 2000). "Nitric-oxide dioxygenase activity and function of flavohemoglobins. sensitivity to nitric oxide and carbon monoxide inhibition". J. Biol. Chem. 275 (41): 31581–7. doi:10.1074/jbc.M004141200. PMID 10922365. 
  8. ^ Hausladen, A., Gow, A. J., and Stamler, J. S. (2001). "Flavohemoglobin denitrosylase catalyzes the reaction of a nitroxyl equivalent with molecular oxygen". PNAS 98 (18): 10108–12. doi:10.1073/pnas.181199698. 
  9. ^ Mills CE, Sedelnikova S, Søballe B, Hughes MN, Poole RK (January 2001). "Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide". Biochem. J. 353 (Pt 2): 207–13. PMC 1221560. PMID 11139382. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221560. 
  10. ^ Gardner PR, Costantino G, Salzman AL (1998). "Constitutive and adaptive detoxification of nitric oxide in Escherichia coli. Role of nitric-oxide dioxygenase in the protection of aconitase". J. Biol. Chem. 273 (41): 26528–33. doi:10.1074/jbc.273.41.26528. PMID 9756889. 
  11. ^ Gardner PR, Gardner AM, Martin LA, Salzman AL (1998). "Nitric oxide dioxygenase: an enzymic function for flavohemoglobin". Proc. Natl. Acad. Sci. U. S. A. 95 (18): 10378–83. doi:10.1073/pnas.95.18.10378. PMC 27902. PMID 9724711. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27902. 
  12. ^ a b c Li BR, Anderson JL, Mowat CG, Miles CS, Reid GA, Chapman SK (October 2008). "Rhodobacter sphaeroides haem protein: a novel cytochrome with nitric oxide dioxygenase activity". Biochem. Soc. Trans. 36 (Pt 5): 992–5. doi:10.1042/BST0360992. PMID 18793176. 
  13. ^ Bartnikas TB, Tosques IE, Laratta WP, Shi J, Shapleigh JP (June 1997). "Characterization of the nitric oxide reductase-encoding region in Rhodobacter sphaeroides 2.4.3". J. Bacteriol. 179 (11): 3534–40. PMC 179145. PMID 9171397. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=179145. 
  14. ^ Gardner AM, Cook MR, Gardner PR (July 2010). "Nitric-oxide dioxygenase function of human cytoglobin with cellular reductants and in rat hepatocytes". J. Biol. Chem. 285 (31): 23850–7. doi:10.1074/jbc.M110.132340. PMC 2911317. PMID 20511233. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2911317. 
  15. ^ Halligan KE, Jourd'heuil FL, Jourd'heuil D (March 2009). "Cytoglobin is expressed in the vasculature and regulates cell respiration and proliferation via nitric oxide dioxygenation". J. Biol. Chem. 284 (13): 8539–47. doi:10.1074/jbc.M808231200. PMC 2659212. PMID 19147491. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2659212. 
  16. ^ Gardner PR (January 2005). "Nitric oxide dioxygenase function and mechanism of flavohemoglobin, hemoglobin, myoglobin and their associated reductases". J. Inorg. Biochem. 99 (1): 247–66. doi:10.1016/j.jinorgbio.2004.10.003. PMID 15598505. 
  17. ^ Gardner PR, Gardner AM, Martin LA, Salzman AL (September 1998). "Nitric oxide dioxygenase: an enzymic function for flavohemoglobin". Proc. Natl. Acad. Sci. U.S.A. 95 (18): 10378–83. doi:10.1073/pnas.95.18.10378. PMC 27902. PMID 9724711. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27902. 
  18. ^ Vinogradov SN, Moens L (April 2008). "Diversity of globin function: enzymatic, transport, storage, and sensing". J. Biol. Chem. 283 (14): 8773–7. doi:10.1074/jbc.R700029200. PMID 18211906. 
  19. ^ Hoppe-Seyler F (1866). "Über die Oxydation in lebenden Blute". Med.-Chem. Untersuch Lab. 1: 133–40. 
  20. ^ Hallstrom CK, Gardner AM, Gardner PR (July 2004). "Nitric oxide metabolism in mammalian cells: substrate and inhibitor profiles of a NADPH-cytochrome P450 oxidoreductase-coupled microsomal nitric oxide dioxygenase". Free Radic. Biol. Med. 37 (2): 216–28. doi:10.1016/j.freeradbiomed.2004.04.031. PMID 15203193. 
  21. ^ Helmick RA, Fletcher AE, Gardner AM, Gessner CR, Hvitved AN, Gustin MC, Gardner PR (May 2005). "Imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin". Antimicrob. Agents Chemother. 49 (5): 1837–43. doi:10.1128/AAC.49.5.1837-1843.2005. PMC 1087630. PMID 15855504. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1087630. 
  22. ^ El Hammi E, Warkentin E, Demmer U, Limam F, Marzouki NM, Ermler U, Baciou L (February 2011). "Structure of Ralstonia eutropha flavohemoglobin in complex with three antibiotic azole compounds". Biochemistry 50 (7): 1255–64. doi:10.1021/bi101650q. PMID 21210640. 
  23. ^ a b Helmick RA, Fletcher AE, Gardner AM, Gessner CR, Hvitved AN, Gustin MC, Gardner PR (May 2005). "Imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin". Antimicrob. Agents Chemother. 49 (5): 1837–43. doi:10.1128/AAC.49.5.1837-1843.2005. PMC 1087630. PMID 15855504. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1087630. 
  24. ^ Ermler U, Siddiqui RA, Cramm R, Friedrich B (December 1995). "Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 Å resolution". EMBO J. 14 (24): 6067–77. PMC 394731. PMID 8557026. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=394731. 
  25. ^ Ollesch G, Kaunzinger A, Juchelka D, Schubert-Zsilavecz M, Ermler U (June 1999). "Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus". Eur. J. Biochem. 262 (2): 396–405. doi:10.1046/j.1432-1327.1999.00381.x. PMID 10336624. 

Wikimedia Foundation. 2010.

Игры ⚽ Нужен реферат?

Look at other dictionaries:

  • List of EC numbers (EC 1) — This list contains a list of EC numbers for the first group, EC 1, oxidoreducatases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.EC 1.1 Acting on the CH OH …   Wikipedia

  • Hemoglobin — Hemoglobin, human, adult (heterotetramer, (αβ)2) Structure of human hemoglobin. The protein s α and β subunits are in red and blue, and the iron containing heme groups in green. Fro …   Wikipedia

  • List of enzymes — This article is a list of enzymes, sorted by their respective sub categories and EC number.See also: * List of EC numbers * List of EC numbers (EC 1) * List of EC numbers (EC 2) * List of EC numbers (EC 3) * List of EC numbers (EC 4) * List of EC …   Wikipedia

  • Endotheliale Stickstoffmonoxid-Synthase — Masse/Länge Primärstruktur 1202 Aminosäuren …   Deutsch Wikipedia

  • Amino acid — This article is about the class of chemicals. For the structures and properties of the standard proteinogenic amino acids, see Proteinogenic amino acid. The generic structure of an alpha amino acid in its unionized form …   Wikipedia

  • Oxidoreductase — In biochemistry, an oxidoreductase is an enzyme that catalyzes the transfer of electrons from one molecule (the reductant, also called the hydrogen or electron donor) to another (the oxidant, also called the hydrogen or electron acceptor). This… …   Wikipedia

  • Phenylalanine hydroxylase — predicted 3D structure of phenylalanine hydroxylase …   Wikipedia

  • Oxygenase — An oxygenase is any enzyme that oxidizes a substrate by transferring the oxygen from molecular oxygen O2 (as in air) to it. The oxygenases form a class of oxidoreductases; their EC number is EC 1.13 or EC 1.14. Oxygenases were discovered in 1955… …   Wikipedia

  • PTGS1 — Prostaglandin endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) PDB rendering based on 1diy …   Wikipedia

  • Metabolism — Cell metabolism redirects here. For the journal, see Cell Metabolism. Structure of adenosine triphosphate, a central intermediate in energy metabolism Metabolism (from Greek: μεταβολή metabolē , change or Greek: μεταβολισμός metabolismos,… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”