Major basic protein

Major basic protein
Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
Symbols PRG2; BMPG; MBP; MBP1; MGC14537
External IDs OMIM605601 MGI103294 HomoloGene2044 GeneCards: PRG2 Gene
RNA expression pattern
PBB GE PRG2 211743 s at tn.png
More reference expression data
Species Human Mouse
Entrez 5553 19074
Ensembl ENSG00000186652 ENSMUSG00000027073
UniProt P13727 Q545D8
RefSeq (mRNA) NM_002728.4 NM_008920.4
RefSeq (protein) NP_002719.3 NP_032946.1
Location (UCSC) Chr 11:
57.15 – 57.16 Mb
Chr 2:
84.82 – 84.82 Mb
PubMed search [1] [2]

Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), also known as PRG2, is a protein which in humans is encoded by the PRG2 gene.[1]



The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.[1]

PRG2 is a 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma.


Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize.

Instead, MBP recognises heparan sulfate proteoglycans. Two crystallographic structures of MBP have been determined.[2][3]


Major basic protein has been shown to interact with Pregnancy-associated plasma protein A.[4][5][6]

See also



  1. ^ a b "Entrez Gene: PRG2 proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)". 
  2. ^ PDB 1h8u; Swaminathan GJ, Weaver AJ, Loegering DA, Checkel JL, Leonidas DD, Gleich GJ, Acharya KR (July 2001). "Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity". J. Biol. Chem. 276 (28): 26197–26203. doi:10.1074/jbc.M100848200. PMID 11319227. 
  3. ^ PDB 2brs; Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR (November 2005). "Eosinophil-granule major basic protein, a C-type lectin, binds heparin". Biochemistry 44 (43): 14152–14158. doi:10.1021/bi051112b. PMID 16245931. 
  4. ^ Overgaard, M T; Haaning J, Boldt H B, Olsen I M, Laursen L S, Christiansen M, Gleich G J, Sottrup-Jensen L, Conover C A, Oxvig C (Oct. 2000). "Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor". J. Biol. Chem. (UNITED STATES) 275 (40): 31128–31133. doi:10.1074/jbc.M001384200. ISSN 0021-9258. PMID 10913121. 
  5. ^ Overgaard, Michael T; Sorensen Esben S, Stachowiak Damian, Boldt Henning B, Kristensen Lene, Sottrup-Jensen Lars, Oxvig Claus (Jan. 2003). "Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment". J. Biol. Chem. (United States) 278 (4): 2106–2117. doi:10.1074/jbc.M208777200. ISSN 0021-9258. PMID 12421832. 
  6. ^ Oxvig, C; Sand O, Kristensen T, Gleich G J, Sottrup-Jensen L (Jun. 1993). "Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein". J. Biol. Chem. (UNITED STATES) 268 (17): 12243–6. ISSN 0021-9258. PMID 7685339. 

External links

Further reading