DDX5

DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Identifiers
Symbols	DDX5; DKFZp434E109; DKFZp686J01190; G17P1; HLR1; HUMP68; p68
External IDs	OMIM: 180630 MGI: 105037 HomoloGene: 6797 GeneCards: DDX5 Gene
EC number	3.6.4.13
Gene Ontology Molecular function • nucleotide binding • transcription cofactor activity • RNA binding • RNA helicase activity • protein binding • ATP binding • ATP-dependent helicase activity • hydrolase activity • hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Cellular component • nucleus • nucleus • nucleolus • ribonucleoprotein complex • catalytic step 2 spliceosome Biological process • nuclear mRNA splicing, via spliceosome • RNA splicing • cell growth Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1655	13207
Ensembl	ENSG00000108654	ENSMUSG00000020719
UniProt	P17844	O35662
RefSeq (mRNA)	NM_004396	NM_007840.3
RefSeq (protein)	NP_004387	NP_031866.2
Location (UCSC)	Chr 17: 59.93 – 59.93 Mb	Chr 11: 106.64 – 106.65 Mb
PubMed search	[1]	[2]

Probable ATP-dependent RNA helicase DDX5 also known as DEAD box protein 5 or RNA helicase p68 is an enzyme that in humans is encoded by the DDX5 gene.^[1]

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.^[1]

Interactions

DDX5 has been shown to interact with:

• AKAP8,^[2]
• DDX17 (p72),^[3]
• DHX9 (RNA helicase A),^[4]
• Estrogen receptor alpha,^[5][6]
• Fibrillarin,^[7]
• HDAC1,^[8]
• Nuclear receptor coactivator 1,^[9]
• Nuclear receptor coactivator 2,^[9]
• Nuclear receptor coactivator 3,^[9]
• p53,^[10]
• CTCF. ^[11]

References

1. ^ ^{a b} "Entrez Gene: DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1655. 
2. ^ Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM (May 2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182. 
3. ^ Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV (March 2003). "The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells". Nucleic Acids Res. 31 (5): 1470–80. doi:10.1093/nar/gkg236. PMC 149829. PMID 12595555. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149829. 
4. ^ Wilson BJ, Giguère V (2007). "Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis". BMC Genomics 8: 419. doi:10.1186/1471-2164-8-419. PMID 18005418. 
5. ^ Endoh H, Maruyama K, Masuhiro Y, Kobayashi Y, Goto M, Tai H, Yanagisawa J, Metzger D, Hashimoto S, Kato S (August 1999). "Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α". Mol. Cell. Biol. 19 (8): 5363–72. PMC 84379. PMID 10409727. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84379. 
6. ^ Rossow KL, Janknecht R (January 2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene 22 (1): 151–6. doi:10.1038/sj.onc.1206067. PMID 12527917. 
7. ^ Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (June 2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID 10837141. 
8. ^ Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV (August 2004). "The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner". BMC Mol. Biol. 5: 11. doi:10.1186/1471-2199-5-11. PMC 514542. PMID 15298701. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514542. 
9. ^ ^{a b c} Watanabe M, Yanagisawa J, Kitagawa H, Takeyama K, Ogawa S, Arao Y, Suzawa M, Kobayashi Y, Yano T, Yoshikawa H, Masuhiro Y, Kato S (March 2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA". EMBO J. 20 (6): 1341–52. doi:10.1093/emboj/20.6.1341. PMC 145523. PMID 11250900. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=145523. 
10. ^ Bates GJ, Nicol SM, Wilson BJ, Jacobs AM, Bourdon JC, Wardrop J, Gregory DJ, Lane DP, Perkins ND, Fuller-Pace FV (February 2005). "The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor". EMBO J. 24 (3): 543–53. doi:10.1038/sj.emboj.7600550. PMC 548656. PMID 15660129. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=548656. 
11. ^ Yao H, Brick K, Evrard Y, Xiao T, Camerini-Otero RD, Felsenfeld G (2010). "Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA". Genes Dev 24 (22): 2543–2555. doi:10.1101/gad.1967810. PMC 2975930. PMID 20966046. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2975930. 

Further reading

• Iggo RD, Jamieson DJ, MacNeill SA, et al. (1991). "p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts". Mol. Cell. Biol. 11 (3): 1326–33. PMC 369403. PMID 1996094. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=369403. 
• Hloch P, Schiedner G, Stahl H (1990). "Complete cDNA sequence of the human p68 protein". Nucleic Acids Res. 18 (10): 3045. doi:10.1093/nar/18.10.3045. PMC 330836. PMID 2349099. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=330836. 
• Ford MJ, Anton IA, Lane DP (1988). "Nuclear protein with sequence homology to translation initiation factor eIF-4A". Nature 332 (6166): 736–8. doi:10.1038/332736a0. PMID 2451786. 
• Iggo R, Gough A, Xu W, et al. (1989). "Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction". Proc. Natl. Acad. Sci. U.S.A. 86 (16): 6211–4. doi:10.1073/pnas.86.16.6211. PMC 297807. PMID 2762324. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=297807. 
• Buelt MK, Glidden BJ, Storm DR (1994). "Regulation of p68 RNA helicase by calmodulin and protein kinase C". J. Biol. Chem. 269 (47): 29367–70. PMID 7525583. 
• Brody LC, Abel KJ, Castilla LH, et al. (1995). "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17". Genomics 25 (1): 238–47. doi:10.1016/0888-7543(95)80131-5. PMID 7774924. 
• Endoh H, Maruyama K, Masuhiro Y, et al. (1999). "Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α". Mol. Cell. Biol. 19 (8): 5363–72. PMC 84379. PMID 10409727. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84379. 
• Rössler OG, Hloch P, Schütz N, et al. (2000). "Structure and expression of the human p68 RNA helicase gene". Nucleic Acids Res. 28 (4): 932–9. doi:10.1093/nar/28.4.932. PMC 102575. PMID 10648785. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=102575. 
• Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID 10837141. 
• Watanabe M, Yanagisawa J, Kitagawa H, et al. (2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA". EMBO J. 20 (6): 1341–52. doi:10.1093/emboj/20.6.1341. PMC 145523. PMID 11250900. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=145523. 
• Akileswaran L, Taraska JW, Sayer JA, et al. (2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182. 
• Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. 
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370266. 
• Liu ZR (2002). "p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site Duplex". Mol. Cell. Biol. 22 (15): 5443–50. doi:10.1128/MCB.22.15.5443-5450.2002. PMC 133941. PMID 12101238. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133941. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Rossow KL, Janknecht R (2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene 22 (1): 151–6. doi:10.1038/sj.onc.1206067. PMID 12527917. 
• Guil S, Gattoni R, Carrascal M, et al. (2003). "Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation". Mol. Cell. Biol. 23 (8): 2927–41. doi:10.1128/MCB.23.8.2927-2941.2003. PMC 152554. PMID 12665590. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=152554. 
• Fujita T, Kobayashi Y, Wada O, et al. (2003). "Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells". J. Biol. Chem. 278 (29): 26704–14. doi:10.1074/jbc.M301031200. PMID 12738788. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.