aryldialkylphosphatase Identifiers EC number 18.104.22.168 CAS number 117698-12-1 Databases IntEnz IntEnz view BRENDA BRENDA entry ExPASy NiceZyme view KEGG KEGG entry MetaCyc metabolic pathway PRIAM profile PDB structures RCSB PDB PDBe PDBsum Gene Ontology AmiGO / EGO Search PMC articles PubMed articles Phosphotriesterase family Structure of organophosphorus hydrolase Identifiers Symbol PTE Pfam PF02126 InterPro IPR001559 PROSITE PDOC01026 SCOP 1dpm Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary
Aryldialkylphosphatase (EC 22.214.171.124) (also known as organophosphoros hydrolase, phosphotriesterase, and paraoxon hydrolase) is an enzyme that hydrolyse organophosphates:
- an aryl dialkyl phosphate + H2O dialkyl phosphate + an aryl alcohol
Thus, the two substrates of this enzyme are aryl dialkyl phosphate and H2O, whereas its two products are dialkyl phosphate and aryl alcohol.
An organophosphate is the general name for esters of phosphoric acid and is one of the organophosphorus compounds. They can be found as part of insecticides, herbicides, and nerve gases, amongst others. Some less-toxic organophosphates can be used as solvents, plasticizers, and EP additives.
Bacteria such as Pseudomonas diminuta harbor a plasmid that carries the gene for aryldialkylphosphatase (EC 126.96.36.199). This enzyme has attracted interest because of its potential use in the detoxification of chemical waste and warfare agents and its ability to degrade agricultural pesticides such as parathion. It act specifically on synthetic organophosphate triesters and phosphorofluoridates. It does not seem to have a natural occurring substrate and may thus have optimally evolved for utilizing paraoxon.
Aryldialkylphosphatase belongs to a family of enzymes that possess a binuclear zinc metal centre at their active site. The two zinc ions are coordinated by six different residues, six of which being histidines.
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