- Orange G
Orange G Identifiers CAS number PubChem ChemSpider KEGG ChEMBL Jmol-3D images Image 1 Properties Molecular formula C16H10N2Na2O7S2 Molar mass 452.38 g/mol Hazards Main hazards R36/37/38, S26, S36 (what is: /?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
It is often combined with other yellow dyes and used to stain erythrocytes in the trichrome methods.
Orange G can be used as a color marker to monitor the process of agarose gel electrophoresis, running approximately at the size of a 50 Base pair (bp) DNA molecule, and polyacrylamide gel electrophoresis. Bromophenol blue and xylene cyanol can also be used for this purpose. (However, the apparent "size" of all these dyes varies according to the concentration of agarose in the gel and the buffer system used, so one should look up the appropriate reference before using the dyes to determine how far a gel has run.)
Despite its two ionizable groups, it shows only two colors in aqueous solution, brilliant orange in neutral and acidic pH or red in pH greater than 9.
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