Bromophenol blue

Bromophenol blue

Chembox new
IUPACName=2,6-dibromo-4- [3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-3-benzo [c] oxathiolyl] phenol
Section1= Chembox Identifiers

Section2= Chembox Properties

Section3= Chembox Hazards

Bromophenol blue ("3',3",5',5"-tetrabromophenolsulfonphthalein") is an acid-base indicator whose useful range as an indicator lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible.

Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of approximately 500 base pairs. Xylene cyanol and Orange G may also be used for this purpose.cite web |url= |title=Agarose gel electrophoresis (basic method) |accessdate=2008-04-07 |format= |work=]

The bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Solutions of the dye therefore are blue. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red color. This phenomenon is called dichromatic colour.cite journal |author=Kreft S, Kreft M |title=Physicochemical and physiological basis of dichromatic colour |journal=Naturwissenschaften |volume=94 |issue=11 |pages=935–9 |year=2007 |pmid=17534588 |doi=10.1007/s00114-007-0272-9]

Bromophenol blue is commonly used in entry-level lab courses to stain proteins in wet-mount slides.cite web |url= |title=PROTEIN GEL ELECTROPHORESIS |accessdate=2008-04-07 |format= |work=]


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  • bromophenol blue — SYN: bromphenol blue. * * * bro·mo·phe·nol blue .brō mō .fē .nōl , .nȯl , fi also brom·phe·nol blue (.)brōm n a dye C19H10Br4O5S obtained as pinkish crystals and used as an acid base indicator …   Medical dictionary

  • bromophenol blue — Dye used as pH indicator: changes from yellow to blue in the range 3.0 4.6 …   Dictionary of molecular biology

  • bromophenol blue — noun a dye used as an acid base indicator (Freq. 1) • Syn: ↑bromphenol blue, ↑tetrabromo phenolsulfonephthalein • Hypernyms: ↑dye, ↑dyestuff, ↑acid base indicator …   Useful english dictionary

  • bromophenol blue — noun An acid base indicator, 2,6 dibromo 4 [3 (3,5 dibromo 4 hydroxyphenyl) 1,1 dioxo 3 benzo[c]oxathiolyl]phenol …   Wiktionary

  • bromphenol blue — A substituted triphenylmethane dye (MW 670, pK 4.0), used as an acid base indicator (yellow at pH less than 3.1, blue at pH more than 4.7); also used for histochemical and electrophoretic demonstration of proteins. SYN: bromophenol blue. * * * an …   Medical dictionary

  • bromphenol blue — noun a dye used as an acid base indicator • Syn: ↑bromophenol blue, ↑tetrabromo phenolsulfonephthalein • Hypernyms: ↑dye, ↑dyestuff, ↑acid base indicator * * * noun see …   Useful english dictionary

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  • Color marker — A Color marker is used to monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) since DNA, RNA, and most proteins are colourless. They are also referred to as tracking dyes. Commonly used color markers… …   Wikipedia

  • Agarose gel electrophoresis — is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter… …   Wikipedia

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