Colipase

colipase
Cartoon diagram of pig colipase (blue) in complex with human pancreatic lipase and a small molecule inhibitor. From PDB 1LPB.
Identifiers
Symbol	CLPS
External IDs	OMIM: 120105 MGI: 88421 HomoloGene: 1383 GeneCards: CLPS Gene
Gene Ontology Molecular function • enzyme activator activity Cellular component • extracellular region Biological process • lipid catabolic process • post-embryonic development • digestion • response to food • positive regulation of catalytic activity Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1208	109791
Ensembl	ENSG00000137392	ENSMUSG00000024225
UniProt	P04118	Q9CQC2
RefSeq (mRNA)	NM_001832	NM_025469
RefSeq (protein)	NP_001823	NP_079745
Location (UCSC)	Chr 6: 35.87 – 35.87 Mb	Chr 17: 28.7 – 28.7 Mb
PubMed search	[1]	[2]

Colipase is a protein co-enzyme required for optimal enzyme activity of pancreatic lipase. It is secreted by the pancreas in an inactive form, procolipase, which is activated in the intestinal lumen by trypsin. Its function is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides.

In humans, the colipase protein is encoded by the CLPS gene.^[1]

Protein domain

Colipase is also a family of evolutionarily related proteins.

Colipase is a small protein cofactor needed by pancreatic lipase for efficient dietary lipid hydrolyisis. Efficient absorption of dietary fats is dependent on the action of pancreatic triglyceride lipase. Colipase binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising an active conformation and considerably increasing the hydrophobicity binding site. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture.^[2][3]

Colipase is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein (Dickkopf), the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. These non-catalytic domains in the latter enzymes are important for interaction with membrane. It has not been established if these domains are also involved in eventual protein cofactor binding as is the case for pancreatic lipase.^[3]

Colipase N-terminal domain Structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate.[4] Identifiers Symbol Colipase Pfam PF01114 InterPro IPR001981 PROSITE PDOC00111 SCOP 1lpb Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary

Colipase C-terminal domain
solution structure of porcine pancreatic procolipase as determined from 1h homonuclear two-and three-dimensional nmr
Identifiers
Symbol	Colipase_C
Pfam	PF02740
InterPro	IPR017914
PROSITE	PDOC00111
SCOP	1lpb
Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary

See also

• Enterostatin

References

1. ^ Davis RC, Xia YR, Mohandas T, Schotz MC, Lusis AJ (May 1991). "Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter". Genomics 10 (1): 262–5. doi:10.1016/0888-7543(91)90509-D. PMID 2045105. 
2. ^ Lowe ME (1997). "Structure and function of pancreatic lipase and colipase". Annu. Rev. Nutr. 17: 141–158. doi:10.1146/annurev.nutr.17.1.141. PMID 9240923. 
3. ^ ^{a b} Verger R, van Tilbeurgh H, Cambillau C, Bezzine S, Carriere F (1999). "Colipase: structure and interaction with pancreatic lipase". Biochim. Biophys. Acta 1441 (2-3): 173–184. PMID 10570245. 
4. ^ Egloff MP, Marguet F, Buono G, Verger R, Cambillau C, van Tilbeurgh H (March 1995). "The 2.46 A resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate". Biochemistry 34 (9): 2751–62. doi:10.1021/bi00009a003. PMID 7893686. 

Further reading

• Weyrich P, Albet S, Lammers R, et al. (2009). "Genetic variability of procolipase associates with altered insulin secretion in non-diabetic Caucasians.". Exp. Clin. Endocrinol. Diabetes 117 (2): 83–7. doi:10.1055/s-2008-1078733. PMID 18726866. 
• Crandall WV, Lowe ME (2001). "Colipase residues Glu64 and Arg65 are essential for normal lipase-mediated fat digestion in the presence of bile salt micelles.". J. Biol. Chem. 276 (16): 12505–12. doi:10.1074/jbc.M009986200. PMID 11278590. 
• Miled N, Canaan S, Dupuis L, et al. (2000). "Digestive lipases: from three-dimensional structure to physiology.". Biochimie 82 (11): 973–86. doi:10.1016/S0300-9084(00)01179-2. PMID 11099794. 
• van Tilbeurgh H, Egloff MP, Martinez C, et al. (1993). "Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography.". Nature 362 (6423): 814–20. doi:10.1038/362814a0. PMID 8479519. 
• Wermter AK, Scherag A, Holter K, et al. (2009). "Procolipase gene: no association with early-onset obesity or fat intake.". Obes Facts 2 (1): 40–4. doi:10.1159/000196379. PMID 20054203. 
• Lindner I, Helwig U, Rubin D, et al. (2005). "Putative association between a new polymorphism in exon 3 (Arg109Cys) of the pancreatic colipase gene and type 2 diabetes mellitus in two independent Caucasian study populations.". Mol Nutr Food Res 49 (10): 972–6. doi:10.1002/mnfr.200500087. PMID 16189801. 
• Sims HF, Lowe ME (1992). "The human colipase gene: isolation, chromosomal location, and tissue-specific expression.". Biochemistry 31 (31): 7120–5. doi:10.1021/bi00146a013. PMID 1643046. 
• Lowe ME, Rosenblum JL, McEwen P, Strauss AW (1990). "Cloning and characterization of the human colipase cDNA.". Biochemistry 29 (3): 823–8. doi:10.1021/bi00455a032. PMID 2337598. 
• van Tilbeurgh H, Bezzine S, Cambillau C, et al. (1999). "Colipase: structure and interaction with pancreatic lipase.". Biochim. Biophys. Acta 1441 (2-3): 173–84. PMID 10570245. 
• D'Silva S, Xiao X, Lowe ME (2007). "A polymorphism in the gene encoding procolipase produces a colipase, Arg92Cys, with decreased function against long-chain triglycerides.". J. Lipid Res. 48 (11): 2478–84. doi:10.1194/jlr.M700371-JLR200. PMID 17715423. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Sternby B, Engström A, Hellman U, et al. (1984). "The primary sequence of human pancreatic colipase.". Biochim. Biophys. Acta 784 (1): 75–80. PMID 6691986. 
• Sias B, Ferrato F, Grandval P, et al. (2004). "Human pancreatic lipase-related protein 2 is a galactolipase.". Biochemistry 43 (31): 10138–48. doi:10.1021/bi049818d. PMID 15287741. 
• Lowe ME (1997). "Structure and function of pancreatic lipase and colipase.". Annu. Rev. Nutr. 17: 141–58. doi:10.1146/annurev.nutr.17.1.141. PMID 9240923. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Sugar IP, Mizuno NK, Momsen MM, et al. (2003). "Regulation of lipases by lipid-lipid interactions: implications for lipid-mediated signaling in cells.". Chem. Phys. Lipids 122 (1-2): 53–64. doi:10.1016/S0009-3084(02)00178-0. PMID 12598038. 
• van Tilbeurgh H, Sarda L, Verger R, Cambillau C (1992). "Structure of the pancreatic lipase-procolipase complex.". Nature 359 (6391): 159–62. doi:10.1038/359159a0. PMID 1522902. 
• Davis RC, Xia YR, Mohandas T, et al. (1991). "Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter.". Genomics 10 (1): 262–5. doi:10.1016/0888-7543(91)90509-D. PMID 2045105. 

External links

• MeSH Colipases

This article includes text from the public domain Pfam and InterPro IPR001981

This enzyme-related article is a stub. You can help Wikipedia by expanding it.v · Categories: Human proteins Protein domains Membrane proteins Enzymes Protein families Enzyme stubs Wikimedia Foundation. 2010. Игры ⚽ Поможем сделать НИР Diplomatic Revolution Dhun Jehangir Ruttonjee Look at other dictionaries: colipase — Protein cofactor for lipase …   Dictionary of molecular biology colipase — noun A protein coenzyme secreted by the pancreas …   Wiktionary colipase — A small protein in pancreatic juice that is essential for the efficient action of pancreatic lipase. This cofactor inhibits the surface denaturation of the lipase. [co + lipase] …   Medical dictionary Lipase — Pankreaslipase Vorhandene Strukturdaten: 1gpl …   Deutsch Wikipedia Pankreaslipase — Vorhandene Strukturdaten …   Deutsch Wikipedia Intestinal juice — (succus entericus) refers to the clear to pale yellow watery secretions from the glands lining the small intestine walls. Secretion is stimulated by the mechanical pressure of partly digested food in the intestine. Its function is to complete the …   Wikipedia Pancreatic lipase family — Pfam box Symbol = Lipase Name = width =250 caption =Complex of human pancreatic lipase with colipase Pfam= PF00151 InterPro= IPR013818 SMART= PROSITE = PDOC00110 SCOP = 1lpa TCDB = OPM family= OPM protein= 1lpa PDB=PDB3|1rp1 :18 353 PDB3|1lpaB:17 …   Wikipedia Fettverdauung — Die Fettverdauung ist die Verdauung von Fetten und fettähnlichen Substanzen im Körper. Die im Zusammenhang mit der Energiebereitstellung relevanten Fette sind die Triglyceride, bestehend aus Glycerin und drei identischen oder verschiedenen… …   Deutsch Wikipedia digestive system, human — Introduction  the system used in the human body for the process of digestion. The human digestive system consists primarily of the digestive tract (alimentary canal), or the series of structures and organs through which food and liquids pass… …   Universalium Fatty acid metabolism — Fatty acids are an important source of energy for many organisms. Excess glucose can be stored efficiently as fat. Triglycerides yield more than twice as much energy for the same mass as do carbohydrates or proteins. All cell membranes are built… …   Wikipedia 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”