Messenger RNA decapping

The process of messenger RNA decapping consists of hydrolysis of the 5' cap structure on the RNA exposing a 5' monophosphate. This 5' monophosphate is a substrate for the exonuclease Xrn1 and the message is quickly destroyed. There are many situations which may lead to the removal of the cap, some of which are discussed below.

Translation and Decay

Inside cells there is a balance between the processes of translation and mRNA decay. Messages which are being actively translated are bound by polysomes and the initiation factors eIF-4E and eIF-4G. This blocks access to the cap by the decapping enzyme DCP2 and protects the message. In nutrient-starvation conditions or viral infection, translation may be compromised and decapping is stimulated. This balance is reflected in the size and abundance of the cytoplasmic structures known as P-bodies ^[1]

Specific decay pathways

A number of specific decay pathways exist that recognize aberrant messages and promote their decapping. Nonsense mediated decay recognizes premature stop codons and promotes decapping as well as decay by the exosome. Certain classes of miRNA have also been shown to stimulate decapping.

References

1. ^ Parker, R.; Sheth, U. (2007), "P Bodies and the Control of mRNA Translation and Degradation" (w), Molecular Cell 25 (5): 635–646, doi:10.1016/j.molcel.2007.02.011, PMID 17349952, http://linkinghub.elsevier.com/retrieve/pii/S1097276507001116