Dendrocollybia

Dendrocollybia
Dendrocollybia racemosa fruit bodies growing on a decayed mushroom
Scientific classification
Kingdom:	Fungi
Division:	Basidiomycota
Subdivision:	Agaricomycotina
Class:	Agaricomycetes
Order:	Agaricales
Family:	Tricholomataceae
Genus:	Dendrocollybia R.H. Petersen & Redhead
Type species
Dendrocollybia racemosa (Pers.) R.H. Petersen & Redhead
Synonyms[3]
Agaricus racemosus Pers. Collybia racemosa (Pers.) Quél. Microcollybia racemosa (Pers.) Lennox[1] Mycena racemosa (Pers.) Gray[2]

Dendrocollybia is a genus of fungus in the Tricholomataceae family of the Agaricales order. It is a monotypic genus, containing the single species Dendrocollybia racemosa, commonly known as the branched Collybia or the branched shanklet. The somewhat rare species is found in the Northern Hemisphere, including the Pacific Northwest region of western North America, and Europe, where it is included in several Regional Red Lists. It usually grows on the decaying fruit bodies of other agarics—such as Lactarius and Russula—although the host mushrooms may be decayed to the point of being difficult to recognize.

Dendrocollybia racemosa fruit bodies have small pale grayish-white or grayish-brown caps up to 1 cm (0.4 in) wide, and thin stems up to 6 cm (2.4 in) long. The species is characterized by its unusual stem, which is covered with short lateral branches. The branches often produce spherical slimeheads of translucent conidiophores on their swollen tips. The conidiophores produce conidia (asexual spores) by mitosis. Because the fungus can rely on either sexual or asexual modes of reproduction, fruit bodies sometimes have reduced or even missing caps. The unusual stems originate from black pea-sized structures called sclerotia. The anamorphic form of the fungus, known as Tilachlidiopsis racemosa, is missing the sexual stage of its life cycle. It is able to reproduce at relatively low temperatures, an adaptation believed to improve its ability to grow quickly and fruit on decomposing mushrooms.

Taxonomy and phylogeny

Collybia tuberosa Collybia cookei Collybia cirrhata Clitocybe dealbata Dendrocollybia racemosa Clitocybe connata Hypsizygus ulmarius Lepista nuda

Phylogeny and relationships of D. racemosa and closely related fungi based on ribosomal DNA sequences.[4]

The genus Dendrocollybia was first described in 2001, to accommodate the species previously known as Collybia racemosa. Before then, the so-named taxon was considered to be one of four species of Collybia, a genus which had itself been redefined and reduced in 1997, when most of its species were transferred to Gymnopus and Rhodocollybia.^[5] C. racemosa was originally described and named Agaricus racemosus by Christian Hendrik Persoon in 1797,^[6] and sanctioned under that name by Elias Magnus Fries in 1821. In his Systema Mycologicum, Fries classified it in his "tribe" Collybia along with all other similar small, white-spored species with a convex cap and a fragile stem.^[7] In 1873 Lucien Quélet raised Fries' tribe Collybia to generic rank.^[8] Samuel Frederick Gray called the species Mycena racemosa in his 1821 Natural Arrangement of British Plants;^[2] both this name and Joanne Lennox's 1979 Microcollybia racemosa are considered synonyms.^[3]

Rolf Singer's fourth edition (1986) of his comprehensive Agaricales in Modern Taxonomy included Collybia racemosa in section Collybia, in addition to the three species that currently comprise the genus Collybia: C. tuberosa, C. cirrhata and C. cookei.^[9] A phylogenetic analysis of the internal transcribed spacer sequences of ribosomal DNA by Karen Hughes and colleagues showed that C. tuberosa, C. cirrhata and C. cookei form a monophyletic group within a larger Lyophyllum–Tricholoma–Collybia clade that includes several species of Lyophyllum, Tricholoma, Lepista, Hypsizygus and the species C. racemosa. Hughes and colleagues were not able to identify a clade that included all four species of Collybia. Restriction fragment length polymorphism analysis of the ribosomal DNA from the four species corroborated the results obtained from phylogenetic analysis. Based on these results, as well as differences in characteristics such as the presence of unique stem projections, fruit body pigmentation, and macrochemical reactions, they circumscribed the new genus Dendrocollybia to contain C. racemosa.^[4]

The fungus is commonly known as the branched Collybia,^[10] or the branched shanklet;^[11] Samuel Gray referred to it as the "racemelike high-stool".^[2] The genus name Dendrocollybia is a combination of the Ancient Greek words dendro-, meaning "tree", and collybia, meaning "small coin". The specific epithet racemosa is from the Latin word racemus—"a cluster of grapes".^[12]

Description

From Esenbeck's Das System der Pilze und Schwämme (1816)

The cap of Dendrocollybia racemosa is typically between 3 to 10 mm (0.1 to 0.4 in) in diameter, and depending on its stage of development, may be conic to convex, or in maturity, somewhat flattened with a slight rounded central elevation (an umbo). The cap surface is dry and opaque, with a silky texture; its color in the center is fuscous (a dusky brownish-gray color), but the color fades uniformly towards the margin. The margin is usually curved toward the gills initially; as the fruit body matures the edge may roll out somewhat, but it also has a tendency to fray or split with age. There may be shallow grooves on the cap that correspond to the position of the gills underneath, which may give the cap edge a crenate (scalloped) appearance. The flesh is very thin (less than 1 mm thick)^[13] and fragile, lacking in color, and has no distinctive odor or taste.^[14] The gills are relatively broad, narrowly attached to the stem (adnexed), spaced closely together, and colored gray to grayish-tan, somewhat darker than the cap.^[15] There are additional gills, called lamellulae, that do not extend all the way to the stem; they are interspersed between the gills and arranged in up to three series (tiers) of equal length.^[13] Occasionally, the fungus produces stems with aborted caps, or with the caps missing entirely.^[10]

“	Stem resembles the raceme of the currant-bush, from whence the berries have been plucked; branches terminated by hyaline beads which disappear.	”
—S.F. Gray, [2]

The stem is 4 to 6 cm (1.6 to 2.4 in) long by 1 mm thick, roughly equal in width throughout, and tapers to a long "root" which terminates in a dull black, roughly spherical sclerotium.^[14] The stem may be buried deeply in its substrate.^[13] The stem surface is roughly the same color as the cap, with a fine whitish powder on the upper surface. In the lower portion, the stem is brownish, and has fine grooves that run lengthwise up and down the surface.^[15] The lower half is covered with irregularly arranged short branch-like protuberances at right angles to the stem that measure 2–3 by 0.5 mm. These projections are cylindrical and tapering, with ends that are covered with a slime head of conidia (fungal spores produced asexually). D. racemosa is the only mushroom species known that forms conidia on side branches of the stem.^[16] The sclerotium from which the stem arises is watery grayish and homogeneous in cross section (not divided into internal chambers), with a thin dull black outer coat, and measures 3 to 6 mm (0.12 to 0.24 in) in diameter.^[14] American mycologist Alexander H. Smith cautioned that novice collectors will typically miss the sclerotium the first time they find the species.^[17] The edibility of D. racemosa is unknown,^[17] but as David Arora says, the fruit bodies are "much too puny and rare to be of value."^[10]

Microscopic characteristics

The spores are narrowly ellipsoid to ovoid, thin-walled, hyaline (translucent), with dimensions of 4–5.5 by 2–3 µm. When stained with Melzer's reagent, the spores turn a light blue color. The basidia (the spore-bearing cells) are four-spored, measure 16–20 by 3.5–4 µm, and taper gradually towards the base. Cystidia are not differentiated in this species. The cap surface is made of a cuticle of radial, somewhat agglutinated, rather coarse hyphae that differ chiefly in size from the underlying tissue—initially 1–3 µm in diameter, becoming 5–7 µm wide in the underlying tissue. The hyphae are clamped, and encrusted with shallow irregularly shaped masses that are most conspicuous in the surface cells. The gill tissue is made of hyphae that project downward from the cap and arranged in a subparallel fashion, meaning that the hyphae are mostly parallel to one another and are slightly intertwined. The hyphae are clamped, with a narrow, branched compact subhymenium (a narrow zone of small, short hyphae immediately beneath the hymenium) composed of hyphae 2–3 µm in diameter. The conidia are 8.5–12 by 4–5 µm, peanut-shaped, non-amyloid (not changing color when stained with Melzer's reagent), clamped, and produced by fragmentation of the coarse mycelium.^[1] Clamp connections are present in the hyphae.^[17] Asexual spores are 10.0–15.5 by 3–4 µm, ellipsoid to oblong, non-amyloid, and contain granular contents.^[13] The grayish color of the fruit bodies is caused by encrusted pigments (crystalline aggregates of pigment molecules, possibly melanin) that occur throughout the tissue of the stem and cap, including the gills; these pigments are absent in Collybia species.^[4]

The lateral projections of the stem form conidia

Similar species

In contrast to the three species of Collybia,^[4] D. racemosa shows negligible reactivity to common chemical tests used in mushroom identification, including aniline, alpha-napthol, guaiacol, sulfoformol, phenol, and phenol-aniline.^[1]

The cortex (outer tissue layer) of the sclerotium can be used as a diagnostic character to distinguish between D. racemosa and small white specimens of Collybia. The hyphae of the cortex of D. racemosa are "markedly angular", in comparison with C. cookei (rounded hyphae) and C. tuberosa (elongated hyphae).^[18] The cortical layer in D. racemosa has an arrangement that is known as textura epidermoidea—with the hyphae arranged like a jigsaw puzzle. Heavy deposits of dark reddish-brown pigment are evident throughout the cortical tissue in or on the walls and the tips of hyphae.^[4] The remaining Collybia species, C. cirrhata, does not form sclerotia.^[18]

Anamorph form

The anamorphic or imperfect fungi are those that seem to lack a sexual stage in their life cycle, and typically reproduce by the process of mitosis in conidia. In some cases, the sexual stage—or teleomorph stage—is later identified, and a teleomorph-anamorph relationship is established between the species. The International Code of Botanical Nomenclature permits the recognition of two (or more) names for one and the same organism, one based on the teleomorph, the other(s) restricted to the anamorph. Tilachlidiopsis racemosa (formerly known as Sclerostilbum septentrionale, described by Alfred Povah in 1932)^[19] was shown to be the anamorphic form of Dendrocollybia racemosa.^[20] The synnemata (reproductive structures made of compact groups of erect conidiophores) produced by T. racemosa always grow on the stem of Dendrocollybia racemosa. The anamorph has an unusually low optimum growth temperature, between 12 and 18 °C (54 and 64 °F), within a larger growth range of 3 and 22 °C (37 and 72 °F). It is thought that this is an adaptation which allows the mycelium to grow quickly and enhance its chances of fruiting on agaric mushrooms, which are generally short-lived.^[21]

Habitat, distribution, and ecology

Russula crassotunicata has been verified as a host for Dendrocollybia.

Dendrocollybia racemosa is a saprobic species, meaning it derives nutrients by breaking down dead or dying tissue. Its fruit bodies grow on the well-decayed remains of agarics, often suspected to be Lactarius or Russula,^[4][10] although the hosts' identity are often unclear due to an advanced state of decay. A 2006 study used molecular analysis to confirm Russula crassotunicata as a host for D. racemosa. This Russula has a long and persistent decay period, and, in the Pacific Northwest region of the United States where the study was conducted, provides a "nearly year-round substrate for mycosaprobic species".^[22] Dendrocollybia is one of four agaric genera obligately associated with growth on the fruit bodies of other fungi, the others being Squamanita, Asterophora, and Collybia.^[4] Dendrocollybia is also found less commonly in deep coniferous duff, in groups or small clusters. The fungus can form sclerotia in the mummified host fruit bodies, and may also develop directly from their sclerotia in soil.^[23] The fungus is widely distributed in temperate regions of the Northern Hemisphere,^[24][25] but rarely collected "probably due to its small size, camouflage color, and tendency to be immersed in its substrate."^[1] In North America, where the distribution is restricted to the Pacific Northwest,^[26] fruit bodies are found in the late summer to autumn, often after a heavy fruiting period for other mushrooms is over.^[17] In Europe, it is known from the United Kingdom, Scandinavia,^[27] and Belgium.^[4] Dendrocollybia racemosa is in the Danish,^[28] Norwegian,^[29] and British Red Lists.^[30]

The saprobic behaviors of Collybia and Dendrocollybia are slightly different. In the autumn, fruit bodies of C. cirrhata, C. cookei and C. tuberosa, can be found on blackened, leathery, mummified fruit bodies of their hosts. Sometimes, these species appear to be growing in the soil (or from their sclerotium in soil or moss), but usually not in huge clusters. In these cases it is assumed that the hosts are remnants of fruit bodies from a previous season. In all observed cases of D. racemosa, however, the hosts have not been readily observed, suggesting that rapid digestion of the host (rather than mummification) may have taken place. Hughes and colleagues suggest that this may indicate the presence of a different enzymatic system, and a differing ability to compete with other fungi or bacteria.^[4]

See also

Fungi portal

• List of Tricholomataceae genera

References

1. ^ ^{a b c d} Lennox JW. (1979). "Collybioid genera in the Pacific Northwest". Mycotaxon 9 (1): 117–231. http://www.cybertruffle.org.uk/cyberliber/59575/0009/001/0117.htm. 
2. ^ ^{a b c d} Gray SF. (1821). A Natural Arrangement of British Plants. London: Baldwin, Cradock, and Joy. p. 620. http://books.google.ca/books?id=YuIYAAAAYAAJ&dq=%22A%20Natural%20Arrangement%20of%20British%20Plants%22%20Volume%201%20Gray&pg=PA620. 
3. ^ ^{a b} "Dendrocollybia racemosa (Pers.) R.H. Petersen & Redhead 2001". MycoBank. International Mycological Association. http://www.mycobank.org/MycoTaxo.aspx?Link=T&Rec=466518. Retrieved 2010-10-22. 
4. ^ ^{a b c d e f g h i} Hughes KW, Petersen RH, Johnson JE, Moncalvo J-E, Vilgalys R, Redhead SA, Thomas T, McGhee LL. (2001). "Infragenic phylogeny of Collybia s. str. based on sequences of ribosomal ITS and LSU regions". Mycological Research 105 (2): 164–72. doi:10.1017/S0953756200003415. 
5. ^ Antonín V, Halling RE, Noordeloos ME. (1997). "Generic concepts within the groups of Marasmius and Collybia sensu lato". Mycotaxon 63: 359–68. http://www.cybertruffle.org.uk/cyberliber/59575/0063/0359.htm. 
6. ^ Persoon CH. (1794). "Dispositio methodica fungorum" (in Latin). Neues Magazin für die Botanik, Römer (Zürich) 1: 81–128. 
7. ^ Fries EM. (1821) (in Latin). Systema Mycologicum. 1. Lundae: Ex Officina Berlingiana. p. 134. http://www.biodiversitylibrary.org/item/25498#198. 
8. ^ Quélet L. (1873). "Les champignons du Jura et des Vosges. IIe Partie [Mushrooms of the Jura and the Vosges. 2nd Part.]" (in French). Mémoires de la Société d'Émulation de Montbéliard. II 5: 333–427. 
9. ^ Singer R. (1986). The Agaricales in Modern Taxonomy (4th ed.). Königstein, Germany: Koeltz Scientific Books. p. 318. ISBN 3-87429-254-1. 
10. ^ ^{a b c d} Arora D. (1986). Mushrooms Demystified: a Comprehensive Guide to the Fleshy Fungi. Berkeley, California: Ten Speed Press. p. 213. ISBN 0-89815-169-4. http://books.google.com/books?id=86tM01VsFG0C&lpg=PA212&dq=collybia%20racemosa&pg=PA213. 
11. ^ "Recommended English Names for Fungi in the UK" (PDF). British Mycological Society. http://www.fungi4schools.org/Reprints/ENGLISH_NAMES.pdf. Retrieved 2010-10-22. 
12. ^ Haubrich WS. (2003). Medical Meanings: A Glossary of Word Origins, (Second ed.). Philadelphia, Pennsylvania: American College of Physicians. p. 201. ISBN 1-930513-49-6. http://books.google.com/books?id=8xCRO8ORO0EC&lpg=PA201&dq=Latin%20racemus&pg=PA201. 
13. ^ ^{a b c d} Wood M, Stevens F. "Collybia racemosa". California Fungi. http://www.mykoweb.com/CAF/species/Dendocollybia_racemosa.html. Retrieved 2010-10-22. 
14. ^ ^{a b c} Smith AH. (1937). "Notes on agarics from the Western United States". Bulletin of the Torrey Botanical Club 64 (7): 477–87. doi:10.2307/2481096. JSTOR 2481096. 
15. ^ ^{a b} Bas C, Kuyper Th W, Noordeloos ME, Vellinga EC, van Os J. (1995). Flora Agaricina Neerlandica. 3. Boca Raton, Florida: CRC Press. pp. 109–10. ISBN 90-5410-616-6. 
16. ^ Castellano MA, Cazares E, Fondrick B, Dreisbach T. (2003) (PDF). Handbook to additional fungal species of special concern in the Northwest Forest Plan (Gen. Tech Rep. PNW-GTR-572). Portland, Oregon: U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station. p. S3–S51. http://www.fs.fed.us/pnw/publications/pnw_gtr572/pnw_gtr572c.pdf. Retrieved 2010-05-12. 
17. ^ ^{a b c d} Smith AH. (1975). A Field Guide to Western Mushrooms. Ann Arbor, Michigan: University of Michigan Press. pp. 108–09. ISBN 0-472-85599-9. 
18. ^ ^{a b} Komorovska H. (2000). "A new diagnostic character for the genus Collybia (Agaricales)". Mycotaxon 75: 343–46. http://www.cybertruffle.org.uk/cyberliber/59575/0075/0343.htm. 
19. ^ Povah AHW. (1932). "New fungi from Isle Royale". Mycologia 24 (2): 240–44. doi:10.2307/3753686. JSTOR 3753686. 
20. ^ Watling R, Kendrick B. (1977). "Dimorphism in Collybia racemosa". Michigan Botanist 16 (2): 65–72. 
21. ^ Stalpers JA, Seifert KA, Samson RA. (1991). "A revision of the genera Antromycopsis, Sclerostilbum, and Tilachlidiopsis (Hyphomycetes)". Canadian Journal of Botany 69 (1): 6–15. doi:10.1139/b91-002. http://rparticle.web-p.cisti.nrc.ca/rparticle/AbstractTemplateServlet?calyLang=eng&journal=cjb&volume=69&year=0&issue=1&msno=b91-002. 
22. ^ Machniki N, Wright LL, Allen A, Robertson CP, Meyer C, Birkebak JM, Ammirati JF. (2006). "Russula crassotunicata identified as a host for Dendrocollybia racemosa" (PDF). Pacific Northwest Fungi 1 (9): 1–7. doi:10.2509/pnwf.2006.001.009. http://www.pnwfungi.org/pdf_files/manuscripts_volume_1/pnwf20069.pdf. 
23. ^ Bills GF, Mueller GM, Foster MS. (2004). Biodiversity of Fungi: Inventory and Monitoring Methods. Amsterdam: Elsevier Academic Press. p. 371. ISBN 0-12-509551-1. http://books.google.com/?id=Du2bi4tgcVcC&lpg=PA371. 
24. ^ Kirk PM, Cannon PF, Minter DW, Stalpers JA. (2008). Dictionary of the Fungi (10th ed.). Wallingford: CABI. p. 198. ISBN 978-0-85199-826-8. 
25. ^ Noordeloos ME. (1995). "Collybia". In Bas C, Kuyper TW, Noordells ME, Vellinga EC. Flora Agaricina Neerlandica: Vol. 3 (Tricholomataceae II). Rotterdam, The Netherlands: A.A. Balkema. pp. 106–23. ISBN 978-90-5410-617-3. 
26. ^ Kuo M. (2005). "Dendrocollybia racemosa". MushroomExpert.Com. http://www.mushroomexpert.com/dendrocollybia_racemosa.html. Retrieved 2010-05-12. 
27. ^ Oregon Biodiversity Information Center. (2010) (PDF). Rare, Threatened and Endangered Species of Oregon (Report). Portland, Oregon: Institute for Natural Resources, Portland State University. p. 91. http://orbic.pdx.edu/documents/2010-rte-book.pdf. 
28. ^ Heilmann-Clausen J. "NERI - The Danish Red Data Book - Dendrocollybia racemosa (Pers.) R.H. Petersen & Redhead". National Environmental Research Institute, Denmark. http://www2.dmu.dk/1_Om_DMU/2_Tvaer-funk/3_fdc_bio/projekter/redlist/data_en.asp?ID=5656&gruppeID=90. Retrieved 2010-05-12. 
29. ^ "Red List of Threatened Fungi in Norway". Norsk Rødliste 2006. The Herbarium, The Natural History Museums and Botanical Garden, University of Oslo. http://www.nhm.uio.no/botanisk/sopp/redgroup.htm. Retrieved 2010-10-26. 
30. ^ Evans S. (2009) (PDF). The Red Data List of Threatened British Fungi (Report). British Mycological Society. http://www.fieldmycology.net/Download/RDL_of_Threatened_British_Fungi.pdf. 

External links

• Dendrocollybia in Index Fungorum.
• Fungi of Poland Several photographs
• National Botanic Garden of Belgium Photo of herbarium specimen