Trypan blue

Trypan blue
Trypan Blue
Identifiers
CAS number 72-57-1 YesY, (tetrasodium salt)
PubChem 5904246
ChemSpider 10482308 YesY
UNII I2ZWO3LS3M YesY
KEGG C19307 YesY
ChEMBL CHEMBL1640
Jmol-3D images Image 1
Properties
Molecular formula C34H28N6O14S4
Molar mass 872.88 g mol−1
 YesY blue (verify) (what is: YesY/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Trypan blue is a vital stain used to selectively colour dead tissues or cells blue. It is a diazo dye.

Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method. This dye may be a cause of certain birth defects such as encephalocele.

Contents

Background & chemistry

Trypan blue is derived from toluidine, that is, any of several isomeric bases, C14H16N2, derived from toluene. Trypan blue is so-called because it can kill trypanosomes, the parasites that cause sleeping sickness. An analog of trypan blue, suramin is used pharmacologically against trypanosomiasis. Trypan blue is also known as diamine blue and Niagara blue.

Trypan red and Trypan blue were first synthesized by the German scientist Paul Ehrlich in 1904.

Uses of trypan blue

Observation with an optical microscope of Hyaloperonospora parasitica within a leaf of Arabidopsis thaliana by using the trypan blue staining.

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells.

It may also be used to observe fungal hyphae and Stramenopiles.

References

Synonyms

  • Azidine Blue 3B
  • Benzamine Blue 3B
  • Benzo Blue 3B
  • Chlorazol Blue 3B
  • Diamine Blue 3B
  • Dianil Blue H3G
  • Direct Blue 14
  • Niagara Blue 3B

External links


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Look at other dictionaries:

  • trypan blue — trypan blue. См. трипановый синий. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Trypan blue — Biological stain used to determine cell viability. Trypan blue is unable to cross intact plasma membranes, and so only labels dead cells …   Dictionary of molecular biology

  • trypan blue — ˈtripən, ˈtriˌpan , trə̇ˈpan noun Etymology: trypan International Scientific Vocabulary trypan ; from its being trypanocidal : a disazo dye derived from tolidine and H acid that has limited use as an intravitam stain and especially formerly in… …   Useful english dictionary

  • trypan blue — noun A blue azo dye that was once used as a trypanocide …   Wiktionary

  • trypan blue — An acid azo dye, used for vital staining of the reticuloendothelial system, uriniferous tubules, and cells in tissue culture, and as an experimental teratogen; formerly used as a trypanocide. * * * tryp·an blue trip ən …   Medical dictionary

  • trypan red — trypan as in trypan blue noun Etymology: trypan International Scientific Vocabulary trypan : a disazo dye derived from benzidine and beta naphthylamine and formerly used as a remedy in trypanosomiasis …   Useful english dictionary

  • blue — Synonyms and related words: Alice blue, Amytal, Amytal pill, Brunswick blue, Caelus, Capri blue, Chinese blue, Copenhagen blue, Demerol, Dolophine, Dresden blue, Fescennine, French blue, Gobelin blue, H, Luminal, Luminal pill, M, Mickey Finn,… …   Moby Thesaurus

  • trypan — noun The dye (anf former drug) trypan blue …   Wiktionary

  • Bleu de trypan — Général Nom IUPAC 3,3 [(3,3 diméthyl[1,1 biphényl] 4,4 diyl)bis(azo)]bis[5 amino 4 hydroxynaphtalène 2,7 disulfonate] de tétrasodium …   Wikipédia en Français

  • Coloration Au Bleu De Trypan — La coloration au bleu de trypan est une méthode de coloration des cellules mortes. Principe Molécule du bleu de trypan Le colorant utilisé, le bleu de trypan, a tendance à entrer dans les cellules qu il rencontre. Une fois dans la cellule, la… …   Wikipédia en Français

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