DAP3

Death associated protein 3
Identifiers
Symbols	DAP3; DAP-3; DKFZp686G12159; FLJ12817; MGC126058; MGC126059; MRP-S29; MRPS29; bMRP-10
External IDs	OMIM: 602074 MGI: 1929538 HomoloGene: 3404 GeneCards: DAP3 Gene
Gene Ontology Molecular function • protein binding Cellular component • nucleus • nucleolus • nucleolus • mitochondrion • mitochondrial ribosome • small ribosomal subunit Biological process • induction of apoptosis by extracellular signals • apoptotic mitochondrial changes Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7818	65111
Ensembl	ENSG00000132676	ENSMUSG00000068921
UniProt	P51398	Q9ER88
RefSeq (mRNA)	NM_001199849.1	NM_022994
RefSeq (protein)	NP_001186778.1	NP_075370
Location (UCSC)	Chr 1: 155.66 – 155.71 Mb	Chr 3: 88.72 – 88.75 Mb
PubMed search	[1]	[2]

28S ribosomal protein S29, mitochondrial is a protein that in humans is encoded by the DAP3 gene.^[1][2][3]

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.^[3]

Interactions

DAP3 has been shown to interact with FADD,^[4] TNFRSF10A,^[4] Glucocorticoid receptor^[5] and Heat shock protein 90kDa alpha (cytosolic), member A1.^[5]

References

1. ^ Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A (Jan 1996). "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death". J Biol Chem 270 (46): 27932–6. doi:10.1074/jbc.270.46.27932. PMID 7499268. 
2. ^ Kissil JL, Kimchi A (Sep 1997). "Assignment of death associated protein 3 (DAP3) to human chromosome 1q21 by in situ hybridization". Cytogenet Cell Genet 77 (3–4): 252. doi:10.1159/000134587. PMID 9284927. 
3. ^ ^{a b} "Entrez Gene: DAP3 death associated protein 3". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7818. 
4. ^ ^{a b} Miyazaki, T; Reed J C (Jun. 2001). "A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins". Nat. Immunol. (United States) 2 (6): 493–500. doi:10.1038/88684. ISSN 1529-2908. PMID 11376335. 
5. ^ ^{a b} Hulkko, S M; Wakui H, Zilliacus J (Aug. 2000). "The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function". Biochem. J. (ENGLAND) 349 Pt 3 (Pt 3): 885–93. ISSN 0264-6021. PMC 1221218. PMID 10903152. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221218. 

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Hulkko SM, Wakui H, Zilliacus J (2001). "The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function". Biochem. J. 349 Pt 3 (Pt 3): 885–93. PMC 1221218. PMID 10903152. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221218. 
• Morgan CJ, Jacques C, Savagner F, et al. (2001). "A conserved N-terminal sequence targets human DAP3 to mitochondria". Biochem. Biophys. Res. Commun. 280 (1): 177–81. doi:10.1006/bbrc.2000.4119. PMID 11162496. 
• Cavdar Koc E, Ranasinghe A, Burkhart W, et al. (2001). "A new face on apoptosis: death-associated protein 3 and PDCD9 are mitochondrial ribosomal proteins". FEBS Lett. 492 (1–2): 166–70. doi:10.1016/S0014-5793(01)02250-5. PMID 11248257. 
• Cavdar Koc E, Burkhart W, Blackburn K, et al. (2001). "The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present". J. Biol. Chem. 276 (22): 19363–74. doi:10.1074/jbc.M100727200. PMID 11279123. 
• Miyazaki T, Reed JC (2001). "A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins". Nat. Immunol. 2 (6): 493–500. doi:10.1038/88684. PMID 11376335. 
• Suzuki T, Terasaki M, Takemoto-Hori C, et al. (2001). "Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28 S small subunit". J. Biol. Chem. 276 (35): 33181–95. doi:10.1074/jbc.M103236200. PMID 11402041. 
• Kenmochi N, Suzuki T, Uechi T, et al. (2001). "The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders". Genomics 77 (1–2): 65–70. doi:10.1006/geno.2001.6622. PMID 11543634. 
• Berger T, Kretzler M (2002). "Interaction of DAP3 and FADD only after cellular disruption". Nat. Immunol. 3 (1): 3–5. doi:10.1038/ni0102-3b. PMID 11753396. 
• Hulkko SM, Zilliacus J (2002). "Functional interaction between the pro-apoptotic DAP3 and the glucocorticoid receptor". Biochem. Biophys. Res. Commun. 295 (3): 749–55. doi:10.1016/S0006-291X(02)00713-1. PMID 12099703. 
• Berger T, Kretzler M (2002). "TRAIL-induced apoptosis is independent of the mitochondrial apoptosis mediator DAP3". Biochem. Biophys. Res. Commun. 297 (4): 880–4. doi:10.1016/S0006-291X(02)02310-0. PMID 12359235. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Zhang Z, Gerstein M (2003). "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome". Genomics 81 (5): 468–80. doi:10.1016/S0888-7543(03)00004-1. PMID 12706105. 
• Mukamel Z, Kimchi A (2004). "Death-associated protein 3 localizes to the mitochondria and is involved in the process of mitochondrial fragmentation during cell death". J. Biol. Chem. 279 (35): 36732–8. doi:10.1074/jbc.M400041200. PMID 15175341. 
• Hirota T, Obara K, Matsuda A, et al. (2004). "Association between genetic variation in the gene for death-associated protein-3 (DAP3) and adult asthma". J. Hum. Genet. 49 (7): 370–5. doi:10.1007/s10038-004-0161-4. PMID 15179560. 
• Miyazaki T, Shen M, Fujikura D, et al. (2004). "Functional role of death-associated protein 3 (DAP3) in anoikis". J. Biol. Chem. 279 (43): 44667–72. doi:10.1074/jbc.M408101200. PMID 15302871. 
• Sasaki H, Ide N, Yukiue H, et al. (2005). "Arg and DAP3 expression was correlated with human thymoma stage". Clin. Exp. Metastasis 21 (6): 507–13. doi:10.1007/s10585-004-2153-3. PMID 15679048.