NEDD4

Neural precursor cell expressed, developmentally down-regulated 4
Rendering based on PDB 2KPZ.
Available structures PDB 2KPZ, 2KQ0, 3B7Y
Identifiers
Symbols	NEDD4; KIAA0093; MGC176705; NEDD4-1; RPF1
External IDs	OMIM: 602278 MGI: 97297 HomoloGene: 88807 GeneCards: NEDD4 Gene
EC number	6.3.2.-
Gene Ontology Molecular function • ubiquitin-protein ligase activity • protein binding • ligase activity • acid-amino acid ligase activity • sodium channel inhibitor activity • protein domain specific binding • beta-2 adrenergic receptor binding • ubiquitin binding • phosphoserine binding • phosphothreonine binding • RNA polymerase binding • proline-rich region binding • proline-rich region binding Cellular component • ubiquitin ligase complex • chromatin • intracellular • membrane fraction • cytoplasm • cytosol • plasma membrane • cell cortex • apicolateral plasma membrane • perinuclear region of cytoplasm Biological process • protein modification process • protein targeting to lysosome • lysosomal transport • nervous system development • negative regulation of sodium ion transport • negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage • positive regulation of phosphatidylinositol 3-kinase cascade • protein ubiquitination • transmission of virus • negative regulation of vascular endothelial growth factor receptor signaling pathway • neuron projection development • receptor internalization • receptor catabolic process • cellular response to UV • protein ubiquitination involved in ubiquitin-dependent protein catabolic process • glucocorticoid receptor signaling pathway • development involved in symbiotic interaction • interspecies interaction between organisms • positive regulation of protein catabolic process • positive regulation of nucleocytoplasmic transport • regulation of dendrite morphogenesis • progesterone receptor signaling pathway • response to calcium ion • protein K63-linked ubiquitination Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4734	17999
Ensembl	ENSG00000069869	ENSMUSG00000032216
UniProt	P46934	P46935
RefSeq (mRNA)	NM_006154.2	NM_010890.3
RefSeq (protein)	NP_006145.2	NP_035020.2
Location (UCSC)	Chr 15: 56.12 – 56.29 Mb	Chr 9: 72.51 – 72.6 Mb
PubMed search	[1]	[2]

E3 ubiquitin-protein ligase NEDD4 also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD-4) is an enzyme that in humans is encoded by the NEDD4 gene.^[1][2]

References

1. ^ Kumar S, Harvey KF, Kinoshita M, Copeland NG, Noda M, Jenkins NA (May 1997). "cDNA cloning, expression analysis, and mapping of the mouse Nedd4 gene". Genomics 40 (3): 435–43. doi:10.1006/geno.1996.4582. PMID 9073511. 
2. ^ Imhof MO, McDonnell DP (Jul 1996). "Yeast RSP5 and its human homolog hRPF1 potentiate hormone-dependent activation of transcription by human progesterone and glucocorticoid receptors". Mol Cell Biol 16 (6): 2594–605. PMC 231250. PMID 8649367. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=231250. 

Further reading

• Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527. 
• Hatakeyama S, Jensen JP, Weissman AM (1997). "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases.". J. Biol. Chem. 272 (24): 15085–92. doi:10.1074/jbc.272.24.15085. PMID 9182527. 
• Gavva NR, Gavva R, Ermekova K, et al. (1997). "Interaction of WW domains with hematopoietic transcription factor p45/NF-E2 and RNA polymerase II.". J. Biol. Chem. 272 (39): 24105–8. doi:10.1074/jbc.272.39.24105. PMID 9305852. 
• Staub O, Gautschi I, Ishikawa T, et al. (1998). "Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination.". EMBO J. 16 (21): 6325–36. doi:10.1093/emboj/16.21.6325. PMC 1170239. PMID 9351815. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170239. 
• Plant PJ, Yeger H, Staub O, et al. (1998). "The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization.". J. Biol. Chem. 272 (51): 32329–36. doi:10.1074/jbc.272.51.32329. PMID 9405440. 
• Ott DE, Coren LV, Copeland TD, et al. (1998). "Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus.". J. Virol. 72 (4): 2962–8. PMC 109742. PMID 9525617. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=109742. 
• Harvey KF, Harvey NL, Michael JM, et al. (1998). "Caspase-mediated cleavage of the ubiquitin-protein ligase Nedd4 during apoptosis.". J. Biol. Chem. 273 (22): 13524–30. doi:10.1074/jbc.273.22.13524. PMID 9593687. 
• Anan T, Nagata Y, Koga H, et al. (1999). "Human ubiquitin-protein ligase Nedd4: expression, subcellular localization and selective interaction with ubiquitin-conjugating enzymes.". Genes Cells 3 (11): 751–63. doi:10.1046/j.1365-2443.1998.00227.x. PMID 9990509. 
• Lu PJ, Zhou XZ, Shen M, Lu KP (1999). "Function of WW domains as phosphoserine- or phosphothreonine-binding modules.". Science 283 (5406): 1325–8. doi:10.1126/science.283.5406.1325. PMID 10037602. 
• Harvey KF, Dinudom A, Komwatana P, et al. (1999). "All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+.". J. Biol. Chem. 274 (18): 12525–30. doi:10.1074/jbc.274.18.12525. PMID 10212229. 
• Morrione A, Plant P, Valentinis B, et al. (1999). "mGrb10 interacts with Nedd4.". J. Biol. Chem. 274 (34): 24094–9. doi:10.1074/jbc.274.34.24094. PMID 10446181. 
• Farr TJ, Coddington-Lawson SJ, Snyder PM, McDonald FJ (2000). "Human Nedd4 interacts with the human epithelial Na+ channel: WW3 but not WW1 binds to Na+-channel subunits.". Biochem. J.. 345 Pt 3 (3): 503–9. doi:10.1042/0264-6021:3450503. PMC 1220784. PMID 10642508. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1220784. 
• Plant PJ, Lafont F, Lecat S, et al. (2000). "Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb.". J. Cell Biol. 149 (7): 1473–84. doi:10.1083/jcb.149.7.1473. PMC 2175132. PMID 10871286. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2175132. 
• Jolliffe CN, Harvey KF, Haines BP, et al. (2001). "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4.". Biochem. J.. 351 Pt 3: 557–65. PMC 1221394. PMID 11042109. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221394. 
• Winberg G, Matskova L, Chen F, et al. (2000). "Latent membrane protein 2A of Epstein-Barr virus binds WW domain E3 protein-ubiquitin ligases that ubiquitinate B-cell tyrosine kinases.". Mol. Cell. Biol. 20 (22): 8526–35. doi:10.1128/MCB.20.22.8526-8535.2000. PMC 102158. PMID 11046148. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=102158. 
• Schubert U, Ott DE, Chertova EN, et al. (2001). "Proteasome inhibition interferes with gag polyprotein processing, release, and maturation of HIV-1 and HIV-2.". Proc. Natl. Acad. Sci. U.S.A. 97 (24): 13057–62. doi:10.1073/pnas.97.24.13057. PMC 27177. PMID 11087859. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27177. 
• Strack B, Calistri A, Accola MA, et al. (2001). "A role for ubiquitin ligase recruitment in retrovirus release.". Proc. Natl. Acad. Sci. U.S.A. 97 (24): 13063–8. doi:10.1073/pnas.97.24.13063. PMC 27178. PMID 11087860. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=27178. 
• Ott DE, Coren LV, Chertova EN, et al. (2001). "Ubiquitination of HIV-1 and MuLV Gag.". Virology 278 (1): 111–21. doi:10.1006/viro.2000.0648. PMID 11112487.