Alpha-enolase

Enolase 1, (alpha)
Rendering based on PDB 2PSN.
Available structures PDB 2PSN, 3B97
Identifiers
Symbols	ENO1; ENO1L1; MPB1; NNE; PPH
External IDs	OMIM: 172430 MGI: 95393 HomoloGene: 68183 GeneCards: ENO1 Gene
EC number	4.2.1.11
Gene Ontology Molecular function • magnesium ion binding • DNA binding • sequence-specific DNA binding transcription factor activity • transcription corepressor activity • phosphopyruvate hydratase activity • protein binding • lyase activity Cellular component • phosphopyruvate hydratase complex • nucleus • cytoplasm • cytosol • plasma membrane • sarcomere Biological process • negative regulation of transcription from RNA polymerase II promoter • carbohydrate metabolic process • glucose metabolic process • gluconeogenesis • glycolysis • transcription, DNA-dependent • response to virus • negative regulation of cell growth • negative regulation of transcription, DNA-dependent Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2023	13806
Ensembl	ENSG00000074800	n/a
UniProt	P06733	n/a
RefSeq (mRNA)	NM_001201483.1	NM_023119.2
RefSeq (protein)	NP_001188412.1	NP_075608.2
Location (UCSC)	Chr 1: 8.92 – 8.94 Mb	n/a
PubMed search	[1]	[2]

Enolase 1, more commonly known as alpha-enolase, is a glycolytic enzyme expressed in most tissues, one of the isozymes of enolase. It is a homodimer composed of 2 alpha subunits.

Relationship to Myc-binding protein-1

Its gene, the ENO1, also encodes the Myc-binding protein-1(MBP1), which downregulates the activity of c-myc protooncogene.^[1] Alpha-enolase is the longer form (48 kDa) localized in both cytoplasm and nuclei, while MBP1 is shorter (37 kDa) and is found mostly in the nuclei.

Clinical significance

Alpha-enolase has been identified as an autoantigen in Hashimoto's encephalopathy.^[2] Single studies have also identified it as an autoantigen associated with severe asthma^[3] and a putative target antigen of anti-endothelial cell antibody in Behçet's disease.^[4] Reduced expression of the enzyme has been found in the corneal epithelium of people suffering from keratoconus.^[5][6]

Interactions

Alpha-enolase has been shown to interact with TRAPPC2.^[7]

See also

• Enolase

External links

• Alpha-Enolase Linked to Severe Asthma - medscape news report, 25 aug 2006.

References

1. ^ Subramanian A, Miller DM. (2000) Structural analysis of alpha-enolase. Mapping the functional domains involved in down-regulation of the c-myc protooncogene. J Biol Chem. 2000 Feb 25;275(8):5958-65. PMID 10681589 free full text
2. ^ Yoneda M, Fujii A, Ito A, Yokoyama H, Nakagawa H, Kuriyama M. High prevalence of serum autoantibodies against the amino terminal of alpha-enolase in Hashimoto's encephalopathy. J Neuroimmunol. 2007 Apr;185(1-2):195-200. Epub 2007 Mar 1. PMID 17335908
3. ^ Nahm DH, Lee KH, Shin JY, Ye YM, Kang Y, Park HS. (2006) Identification of alpha-enolase as an autoantigen associated with severe asthma. J Allergy Clin Immunol. 2006 Aug;118(2):376-81. Epub 2006 Jun 9. PMID 16890761 doi:10.1016/j.jaci.2006.04.002
4. ^ Lee KH, Chung HS, Kim HS, Oh SH, Ha MK, Baik JH, Lee S, Bang D (2003) Human alpha-enolase from endothelial cells as a target antigen of anti-endothelial cell antibody in Behcet's disease. Arthritis Rheum 2003, 48:2025-2035. PMID 12847697 free full text
5. ^ Srivastava OP, Chandrasekaran D, Pfister RR. Molecular changes in selected epithelial proteins in human keratoconus corneas compared to normal corneas. Mol Vis. 2006 Dec 20;12:1615-25. PMID 17200661 free full text
6. ^ Nielsen K, Vorum H, Fagerholm P, Birkenkamp-Demtroder K, Honore B, Ehlers N, Orntoft TF. Proteome profiling of corneal epithelium and identification of marker proteins for keratoconus, a pilot study. Exp Eye Res 2006; 82:201-9. PMID 16083875
7. ^ Ghosh, A K; Majumder M, Steele R, White R A, Ray R B (Jan. 2001). "A novel 16-kilodalton cellular protein physically interacts with and antagonizes the functional activity of c-myc promoter-binding protein 1". Mol. Cell. Biol. (UNITED STATES) 21 (2): 655–62. doi:10.1128/MCB.21.2.655-662.2001. ISSN 0270-7306. PMC 86643. PMID 11134351. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86643.