Lucilia cuprina

Lucilia cuprina
Scientific classification
Kingdom:	Animalia
Phylum:	Arthropoda
Class:	Insecta
Order:	Diptera
Superfamily:	Oestroidea
Family:	Calliphoridae
Genus:	Lucilia
Species:	L. cuprina
Binomial name
Lucilia cuprina (Wiedemann, 1830)
Synonyms
Phaenicia cuprina (Wiedemann, 1830) Chloromelas gorgonea Lindner, 1949 Chloromelas heteroneura f. gorgonea Lindner, 1949 Odontomyia heteronevra Macquart, 1838 Stratiomyia heteroneura Walker, 1854 Stratiomys cuprina Wiedemann, 1830

The species Lucilia cuprina, formerly named Phaenicia cuprina, is more commonly known as the Australian sheep blowfly. It belongs to the blow-fly family Calliphoridae, and while some authorities combine the blow flies (Calliphoridae) and the flesh flies (Sarcophagidae) together into the Metopiidae family, distinguishable characteristics allow for the separation. Lucilia cuprina is a member of the order Diptera, has a pair of short but very strong membranous wings and a pair of hindwings reduced and modified to clublike balancers known as halteres. This adaptation distinguishes the true flies (Diptera) from the other orders with “fly” in their name, and serves as a sensory aid that acts as tiny gyroscopes or inertial navigation system. The mesothorax is much enlarged while the prothorax and metathorax are reduced.^[1] Blow flies are about the size of a house fly or a little larger, and though considered to be nuisances indoors are essential agents in the breakdown of organic matter. Most are metallic blue or green, and oftentimes have black gena, mostly white calypteres and orange yellow anterior thoracic spiracles.

Lucilia cuprina also causes the condition known as 'sheep strike'. The female fly locates a sheep with an open wound in which she lays her eggs. The emerging larvae cause large lesions on the sheep, which may prove to be fatal.

Anatomy

Lucilia cuprina is a species of blow fly characterized by a metallic outer appearance and reddish eyes. They usually have a shiny green or greenish/blue abdomen with bronze/coppery reflections. Because of this, Lucilia species are known as the bronze bottle flies.^[2] Their body shape is round to oval and their length varies from 4.5–10 millimeters. They have two pairs of wings, the first pair being membranous wings and the second pair being reduced wings known as halteres which are used for flight stabilization.^[3] Adults are easy to distinguish due to bristles on the meron, in addition to the arista, the prominent hair on the terminal antennal segment being plumose, or feathery. L. cuprina are most easily identified by their strong dorsal setae and their black thoracic spiracle.

Habitats/diet

Although known as the Australian sheep blowfly, Lucilia cuprina can be found in other parts of the world including Africa and North America. These blow flies like warmer weather with their optimal temperature being around 85 °F (29 °C). Adapted perfectly to the southern region of the United States, this fly is well known because of its importance in forensic entomology. L. cuprina can fly up to ten miles looking for food, and can be found on anything ranging from carrion to decaying fruit. Larvae are often found in shaded regions of carrion, while the adults prefer bright, open areas. ^[4]

Stages of life

Lucilia cuprina, like all flies are holometabolous, meaning they go through a complete metamorphosis. ^[4]

Flies have four stages of growth: egg, larvae, pupa, and adult. Adult L. cuprina arrive early on carrion, appearing hours or even minutes after death. There, on the fresh body, they lay their eggs. The eggs then hatch into larvae which begin to feed and grow. After about five days, larvae enter the pupal stage. This is said to be an inactive stage, although many changes occur during this part of the flies’ life cycle. The pupa does not feed, but rather uses the time inside the casing to change from rice-like larvae into an adult fly with wings and six legs. The whole process can take anywhere from eleven to twenty-one days depending on environmental conditions including temperature and nutritional availability. In most cases warmer temperatures and better nutrition lead to a faster life cycle. L. cuprina can have between four and eight generations per year depending mostly on temperature.

Effects on sheep

Blowfly strike, or flystrike is a serious welfare problem in the animal industry^[5]. This cutaneous myiasis or infestation not only causes severe discomfort or stress to the animal, but will also cause death when left untreated.^[6] Ewe lambs and female sheep are primarily affected and are struck predominately in the rear quadrant of the animal due to fecal staining. Due to the difficulty in controlling these flies, there are considerable losses in the sheep industry every year. Also, there is an increasing concern of the insecticidal use and the surgical procedures done to control L. cuprina, making this not only an animal welfare issue but also an economical one. The maggots of L. cuprina rapidly grow while eating the living flesh of the sheep while secreting ammonia, thus, poisoning the sheep. Sheep show signs of skin irritation by rubbing and biting the affected areas during the first few days after the eggs have been laid. This causes an inflammatory response in the sheep resulting in severe irritation and pyrexia. Once a flystrike has started other flies are attracted to the site. Although treatment is available, the delayed response time due to symptoms allows wool breakage in the affected area and fleece to be tender overall. There are many predispositions to the flystrike that make a host more favorable, including an infection with dermatophilosis and footrot, both of which can be treated and prevented. In some animals a weak resistance can develop, but this immune response is often associated with a decrease in productivity which is an undesirable trait.

Prevention

There are many options available to prevent infestation. Many of the precursors drawing the flies initially are sanitary problems which is where control measures are directed. Drenching, shearing or crutching are some basic procedures that can be done. Crutching is the trimming of excess wool from the breech area, and the timing of both shearing and crutching is critical in reducing the amount of flystrike.

Surgical procedures are also performed in the sheep industry to help prevention, one of which is controversial due to the invasive nature. Tail docking to the correct length will reduce the amount of staining in the breech area due to urine and fecal matter. Pizzle dropping is a procedure that severs the connective tissue between the penis and the body. Not only does it reduce the incidence of relentless pizzle rot in sheep, but it decreases the amount of urine staining on the belly of the sheep. This procedure can greatly reduce the occurrence of flystrike on the belly area. Mulesing is an animal husbandry procedure that has currently faced much opposition throughout its practice. It increases the amount of bare skin around the vulva during the healing process, reducing urine staining and amount of flystrike. This procedure does cause pain, but since it is considered the most effective method to prevent breech strike, it is seemingly justifiable.

Insecticides have also been used often in prevention, but with improper application and heavy reliance throughout the years, insecticide resistance and residues within the wool have caused much concern. The primary reason for failures in using insecticides is attributed to poor application. Jetting, dipping, and backlining are the three most commonly used methods for insecticide application, and most of the chemicals used belong to three types of chemicals: synthetic pyrethroids, organophosphates, and insect growth regulators. Insect growth regulators can provide the long term protection against flies and when applied correctly provide protection during the susceptible times of the year. Many government agencies mandate that the wool be free of insecticidal residues forcing withholding periods by farmers before shearing. During this time, the flock can become extremely sensitive to flystrike.

Baited traps are an alternative to invasive procedures. Unfortunately, baiting is time consuming and must release odors over an extended period of time.^[7] The most common type of bait are offal traps, there have been many attempts to find an alternative.^[8] Vaccinations are currently being developed to help, but none has yet proved effective in the prevention of flystrike.

Demographics

Today Lucilia cuprina can be found throughout the world in various warm locations. Australia is one of the many places L. cuprina are found, and the place where it has been known to cause the most havoc. Their wide distribution is due to movement patterns and the traveling of humans and livestock within the last century. Although they can now be found worldwide, the flies’ origins are linked to afrotropical and oriental regions of the world.

L. cuprina cuprina is distributed in Neotropical, Oriental and Southern Neartic Regions, while L. cuprina dorsalis is found in Australasian, East and Sub-Saharan Afro tropical Regions.^[9].

Similar species

Lucilia cuprina is one of many species of the family Calliphoridae. Though many of its species have similar characteristics, L. cuprina’s closest relative is its sister species, Lucilia sericata. These flies are very similar in appearance and morphological characteristics, which can sometimes cause errors when trying to differentiate between them. They each exhibit specific genetic variations which can be distinguished by using random amplified polymorphic DNA and/or mitochondrial DNA sequences, and are known to cause myiasis (flystrike) in sheep.^[9] They are both one of the first blow flies to arrive at a corpse and each have smooth larva. Unlike L. cuprina, L. sericata does not usually infest live sheep. L. cuprina is a worldwide sheep pest though it is usually found in dry climates. L. sericata has a coastal distribution.^[10]

Forensic importance

Lucilia cuprina is often used as a very helpful tool to aid medical and forensic professionals. It is known to be one of the first flies to occupy a corpse upon its death. Once it lands on a corpse, it continues in the formation of its next generation by laying its eggs. The eggs are followed by its larva, pupa and finally the adult. Forensic professionals may form a postmortem interval by the life stage found on the corpse. L. cuprina, although it is a worldwide pest, is very climate specific. It occupies dryer climates. A forensic investigator may conclude that a corpse has been relocated from its original location if it is found in a moist climate with L. cuprina on it.

The maggots of L. cuprina have been used by medical doctors for Debridement therapy for patients who suffer from wounds that are healing slowly.^[11] The maggots cleanse the wound by eating the dead and infectious skin and preventing gangrene and further infection.

Ongoing research

Current research involving Lucilia cuprina and other Lucilia species range from identifying genetic variation between the different species to the ultrastructure of flies’ eggs. Egg ultrastructure has recently become exceedingly important in the field of forensic science. It is used to distinguish L. cuprina eggs from other Lucilia species, such as Lucilia illustris and Lucilia sericata. This defining feature becomes relevant when determining the post-mortem interval because it will vary with each species.^[12] Other ongoing research includes bacteria and fungi associated with the insect. Numerous studies have been conducted to determine if the fly is a mechanical vector of bacteria. So far, many have been found only to be carriers and cannot transmit disease.^[13] There have also been studies on the taxonomic grouping of Lucilia based on geography. The use of RAPD (Random amplified polymorphic DNA analysis) and mitochondrial DNA sequencing has been used to investigate genetic variation within the species.^[9]

Lucilia cuprina is a commonly used species in the field of forensic entomology. The information known about the species designates L. cuprina as a helpful resource at crime scenes as well as a nuisance to sheep. Ongoing research is constantly finding more effective ways to put the species to use.

References

1. ^ O'Toole, C. (Ed.). (2002). Flies. In Firefly encyclopedia of insects and spiders (pp. 130-145). Firefly Books.
2. ^ Drees, B. M. & Jackman, J. A. (1998). Blow flies. In a field guide to common Texas insects (p. 219). Houston, Texas: Gulf Publishing Company.
3. ^ Durden, C. (1999). Two-wing flies. In G. Zappler (Ed.), Texas insects (pp. 46-49). Austin, Texas: Texas Parks and Wildlife Press.
4. ^ ^{a b} Byrd, J. H., & Castner, J. L. (Eds.). (2001). Insects of forensic importance. In Forensic entomologist: The utility of arthropods in legal investigations (Phaenicia cuprina). Florida: CRC Press.
5. ^ A. C. Heath & D. M. Bishop (2006). "Flystrike in New Zealand: An overview based on a 16-year study, following the introduction and dispersal of the Australian sheep blowfly, Lucilia cuprina Wiedemann (Dipteran: Calliphoridae)". Veterinary Parasitology 137 (3-4): 333–344. doi:10.1016/j.vetpar.2006.01.006. PMID 16464534. 
6. ^ J. W. Plant (2006). "Sheep ectoparasite control and animal welfare". Small Ruminant Research 62: 109–112. doi:10.1016/j.smallrumres.2005.08.003. 
7. ^ Morris, Michael C. “Tests on new bait for flies (Dipteran: Calliphoridae) causing cutaneous myiasis (flystrike) in sheep.” New Zealand Journal of Agricultural Research 48 (2005):151-156
8. ^ Tellman, R.L. Eisemann, C.H. “Inhibition of growth of Lucilia cuprina using serum from sheep vaccinated with first-instar larval antigens.” International Journal of Parasitology 28 (1998):439-450
9. ^ ^{a b c} Jamie Stevens & Richard Wall (1997). "Genetic variation in populations of the blowflies Lucilia cuprina and Lucilia sericata (Diptera: Calliphoridae). Random amplified polymorphic DNA analysis and mitochondrial DNA sequences". Biochemical Systematics and Ecology 25 (2): 81–87. doi:10.1016/S0305-1978(96)00038-5. 
10. ^ "Decomposition: Australian Sheep Blowfly." Australian Sheep Blowfly Lucilia cuprina. 14 Apr. 2009 <http://www.deathonline.net/decomposition/corpse_fauna/flies/l_cuprina.htm>.
11. ^ Mohd Marsi, S., W.A. Nazni. "Sterilisation of Lucilia cuprina Wiedemann Maggot Used in Therapy of Intractable Wounds." Tropical Biomedicine. 22 (2005):185-89. available online: www.msptm.org/files/185_-_189_sterilisation_of_lucilia.pdf
12. ^ Sukontason, K.L. Bunchu, N. Chaiwong, T. Kuntalue, B. Sukontason, K. “Fine structure of the eggshell of the blow fly, Lucilia cuprina.” 8pp. Journal of Insect Science 7:09 (2007), available online: insectscience.org/7.09
13. ^ Banjo, A.D. Lawal, O.A. and Akintola, O.I. “Bacteria and Fungi Associated with Lucilia cuprina (Sheep Blowfly) Larvae.” Research Journal of Agriculture and Biological Sciences 2.6 (2006): 358-364