NONO

Non-POU domain containing, octamer-binding
PDB rendering based on 2cpj.
Available structures PDB 2cpj
Identifiers
Symbols	NONO; NMT55; NRB54; P54; P54NRB
External IDs	OMIM: 300084 MGI: 1855692 HomoloGene: 7212 GeneCards: NONO Gene
Gene Ontology Molecular function • nucleotide binding • DNA binding • RNA binding • protein binding • identical protein binding Cellular component • nucleus • nuclear matrix • paraspeckles Biological process • DNA repair • DNA recombination • regulation of transcription, DNA-dependent • mRNA processing • RNA splicing Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4841	53610
Ensembl	ENSG00000147140	ENSMUSG00000031311
UniProt	Q15233	Q3TF40
RefSeq (mRNA)	NM_001145408.1	NM_023144.2
RefSeq (protein)	NP_001138880.1	NP_075633.2
Location (UCSC)	Chr X: 70.5 – 70.52 Mb	Chr X: 98.62 – 98.64 Mb
PubMed search	[1]	[2]

Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene.^[1][2][3]

Interactions

NONO has been shown to interact with SFPQ,^[4] SPI1^[5] and Androgen receptor.^[6]

References

1. ^ Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res 21 (17): 4085–92. doi:10.1093/nar/21.17.4085. PMC 310009. PMID 8371983. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310009. 
2. ^ Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn Mol Pathol 6 (4): 209–21. doi:10.1097/00019606-199708000-00005. PMID 9360842. 
3. ^ "Entrez Gene: NONO Non-POU domain containing, octamer-binding". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4841. 
4. ^ Peng, Rui; Dye Billy T, Pérez Ismael, Barnard Daron C, Thompson Amanda B, Patton James G (Oct. 2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA (United States) 8 (10): 1334–47. doi:10.1017/S1355838202022070. ISSN 1355-8382. PMC 1370341. PMID 12403470. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370341. 
5. ^ Hallier, M; Tavitian A, Moreau-Gachelin F (May. 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. (UNITED STATES) 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. ISSN 0021-9258. PMID 8626664. 
6. ^ Ishitani, Ken; Yoshida Tasuku, Kitagawa Hirochika, Ohta Hiroaki, Nozawa Shiro, Kato Shigeaki (Jul. 2003). "p54nrb acts as a transcriptional coactivator for activation function 1 of the human androgen receptor". Biochem. Biophys. Res. Commun. (United States) 306 (3): 660–5. doi:10.1016/S0006-291X(03)01021-0. ISSN 0006-291X. PMID 12810069. 

Further reading

• Shav-Tal Y, Zipori D (2002). "PSF and p54(nrb)/NonO--multi-functional nuclear proteins". FEBS Lett. 531 (2): 109–14. doi:10.1016/S0014-5793(02)03447-6. PMID 12417296. 
• Zhang WW, Zhang LX, Busch RK, et al. (1993). "Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells". Biochem. J. 290 ( Pt 1) (Pt 1): 267–72. PMC 1132410. PMID 8439294. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1132410. 
• Hallier M, Tavitian A, Moreau-Gachelin F (1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664. 
• Peters U, Haberhausen G, Kostrzewa M, et al. (1997). "AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism". Hum. Genet. 100 (5–6): 569–72. doi:10.1007/s004390050553. PMID 9341872. 
• Clark J, Lu YJ, Sidhar SK, et al. (1997). "Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma". Oncogene 15 (18): 2233–9. doi:10.1038/sj.onc.1201394. PMID 9393982. 
• Straub T, Grue P, Uhse A, et al. (1998). "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction". J. Biol. Chem. 273 (41): 26261–4. doi:10.1074/jbc.273.41.26261. PMID 9756848. 
• Karhumaa P, Parkkila S, Waheed A, et al. (2000). "Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase". J. Biol. Chem. 275 (21): 16044–9. doi:10.1074/jbc.275.21.16044. PMID 10821857. 
• Straub T, Knudsen BR, Boege F (2000). "PSF/p54(nrb) stimulates "jumping" of DNA topoisomerase I between separate DNA helices". Biochemistry 39 (25): 7552–8. doi:10.1021/bi992898e. PMID 10858305. 
• Mathur M, Tucker PW, Samuels HH (2001). "PSF Is a Novel Corepressor That Mediates Its Effect through Sin3A and the DNA Binding Domain of Nuclear Hormone Receptors". Mol. Cell. Biol. 21 (7): 2298–311. doi:10.1128/MCB.21.7.2298-2311.2001. PMC 86864. PMID 11259580. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86864. 
• Zhang Z, Carmichael GG (2001). "The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs". Cell 106 (4): 465–75. doi:10.1016/S0092-8674(01)00466-4. PMID 11525732. 
• Pavao M, Huang YH, Hafer LJ, et al. (2002). "Immunodetection of nmt55/p54nrb isoforms in human breast cancer". BMC Cancer 1: 15. doi:10.1186/1471-2407-1-15. PMC 59838. PMID 11710964. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=59838. 
• Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. 
• Sewer MB, Nguyen VQ, Huang CJ, et al. (2002). "Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription". Endocrinology 143 (4): 1280–90. doi:10.1210/en.143.4.1280. PMID 11897684. 
• Ohta S, Shiomi Y, Sugimoto K, et al. (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929. 
• Emili A, Shales M, McCracken S, et al. (2002). "Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD". RNA 8 (9): 1102–11. doi:10.1017/S1355838202025037. PMC 1370324. PMID 12358429. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370324. 
• Peng R, Dye BT, Pérez I, et al. (2002). "PSF and p54nrb bind a conserved stem in U5 snRNA". RNA 8 (10): 1334–47. doi:10.1017/S1355838202022070. PMC 1370341. PMID 12403470. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370341. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.