PCBP2

Poly(rC) binding protein 2
PDB rendering based on 1ztg.
Available structures PDB 2AXY, 2JZX, 2P2R, 2PQU, 2PY9
Identifiers
Symbols	PCBP2; HNRPE2; MGC110998; hnRNP-E2
External IDs	OMIM: 601210 MGI: 108202 HomoloGene: 74536 GeneCards: PCBP2 Gene
Gene Ontology Molecular function • DNA binding • RNA binding • protein binding • enzyme binding • ubiquitin protein ligase binding Cellular component • nucleus • nucleoplasm • cytoplasm • cytoplasm • cytosol • ribonucleoprotein complex Biological process • nuclear mRNA splicing, via spliceosome • RNA splicing • response to virus • gene expression • mRNA metabolic process • negative regulation of type I interferon production • proteasomal ubiquitin-dependent protein catabolic process • innate immune response • negative regulation of defense response to virus Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5094	18521
Ensembl	ENSG00000197111	ENSMUSG00000056851
UniProt	Q15366	Q3TF69
RefSeq (mRNA)	NM_001098620.2	NM_011042
RefSeq (protein)	NP_001092090.1	NP_035172
Location (UCSC)	Chr 12: 53.84 – 53.87 Mb	Chr 15: 102.3 – 102.33 Mb
PubMed search	[1]	[2]

Poly(rC)-binding protein 2 is a protein that in humans is encoded by the PCBP2 gene.^[1]

The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene.^[2]

In humans, the PCBP2 gene overlaps with TUC338, a transcribed ultra-conserved element implicated in Hepatocellular carcinoma.^[3]

Interactions

PCBP2 has been shown to interact with HNRPK,^[4] PTBP1^[4] and HNRNPL.^[4]

References

1. ^ Tommerup N, Leffers H (May 1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A". Genomics 32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID 8833161. 
2. ^ "Entrez Gene: PCBP2 poly(rC) binding protein 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5094. 
3. ^ Braconi, C; Valeri, N, Kogure, T, Gasparini, P, Huang, N, Nuovo, GJ, Terracciano, L, Croce, CM, Patel, T (2011 Jan 11). "Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma.". Proceedings of the National Academy of Sciences of the United States of America 108 (2): 786–91. doi:10.1073/pnas.1011098108. PMC 3021052. PMID 21187392. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3021052. Retrieved 17 August 2011. 
4. ^ ^{a b c} Kim, J H; Hahm B, Kim Y K, Choi M, Jang S K (May. 2000). "Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm". J. Mol. Biol. (ENGLAND) 298 (3): 395–405. doi:10.1006/jmbi.2000.3687. ISSN 0022-2836. PMID 10772858. 

Further reading

• Kiledjian M, Wang X, Liebhaber SA (1995). "Identification of two KH domain proteins in the alpha-globin mRNP stability complex.". EMBO J. 14 (17): 4357–64. PMC 394520. PMID 7556077. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=394520. 
• Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.". Eur. J. Biochem. 230 (2): 447–53. doi:10.1111/j.1432-1033.1995.tb20581.x. PMID 7607214. 
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Blyn LB, Swiderek KM, Richards O, et al. (1996). "Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 11115–20. doi:10.1073/pnas.93.20.11115. PMC 38293. PMID 8855318. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=38293. 
• Funke B, Zuleger B, Benavente R, et al. (1996). "The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins". Nucleic Acids Res. 24 (19): 3821–8. doi:10.1093/nar/24.19.3821. PMC 146158. PMID 8871564. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=146158. 
• Blyn LB, Towner JS, Semler BL, Ehrenfeld E (1997). "Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA". J. Virol. 71 (8): 6243–6. PMC 191892. PMID 9223526. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=191892. 
• Kiledjian M, DeMaria CT, Brewer G, Novick K (1997). "Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex". Mol. Cell. Biol. 17 (8): 4870–6. PMC 232339. PMID 9234743. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=232339. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Czyzyk-Krzeska MF, Bendixen AC (1999). "Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA". Blood 93 (6): 2111–20. PMID 10068686. 
• Wang Z, Day N, Trifillis P, Kiledjian M (1999). "An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro". Mol. Cell. Biol. 19 (7): 4552–60. PMC 84253. PMID 10373504. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84253. 
• Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog". J. Biol. Chem. 274 (35): 24849–57. doi:10.1074/jbc.274.35.24849. PMID 10455157. 
• Kim JH, Hahm B, Kim YK, et al. (2000). "Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm". J. Mol. Biol. 298 (3): 395–405. doi:10.1006/jmbi.2000.3687. PMID 10772858. 
• Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins". Genomics 67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID 10936052. 
• Chang CJ, Luh HW, Wang SH, et al. (2002). "The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein". DNA Cell Biol. 20 (9): 569–77. doi:10.1089/104454901317094981. PMID 11747608. 
• Yeap BB, Voon DC, Vivian JP, et al. (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA". J. Biol. Chem. 277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID 12011088. 
• Walter BL, Parsley TB, Ehrenfeld E, Semler BL (2002). "Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication". J. Virol. 76 (23): 12008–22. doi:10.1128/JVI.76.23.12008-12022.2002. PMC 136902. PMID 12414943. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=136902. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Abraham RS, Geyer SM, Price-Troska TL, et al. (2003). "Immunoglobulin light chain variable (V) region genes influence clinical presentation and outcome in light chain-associated amyloidosis (AL)". Blood 101 (10): 3801–8. doi:10.1182/blood-2002-09-2707. PMID 12515719. 
• Pickering BM, Mitchell SA, Evans JR, Willis AE (2003). "Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo". Nucleic Acids Res. 31 (2): 639–46. doi:10.1093/nar/gkg146. PMC 140511. PMID 12527772. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=140511.