Phosphatidic acid

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OtherNames = 1,2-Diacylglycerol 3-phosphate, phosphatidic acid
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PubChem = 5460104
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MeSHName = Phosphatidic+Acids
Section2 = Chembox Properties
Formula = C5H9O8P
MolarMass = 228.094
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Phosphatidic acid (PA) is the acid form of phosphatidate, a common phospholipid that is a major constituent of cell membranes. Phosphatidate acid is the smallest of the phospholipids.

tructure

Phosphatidic acid consists of a glycerol backbone, with, in general, a saturated fatty acid bonded to carbon-1, an unsaturated fatty acid bonded to carbon-2, and a phosphate group bonded to carbon-3.

CH₂-OP(O)(OH)₂ with R₁ and R₂ two acyl chains.
R₂-CO-O-CH
R₁-CO-O-CH₂

Formation and degradation

Besides de novo synthesis, PA can be formed in three ways:
* By phospholipase D (PLD), via the hydrolysis of the P-O bond of phosphatidylcholine (PC) to produce PA and choline.cite journal |author=Liscovitch M, Czarny M, Fiucci G, Tang X |title=Phospholipase D: molecular and cell biology of a novel gene family |journal=Biochem. J. |volume=345 Pt 3 |issue= |pages=401–15 |year=2000 |month=February |pmid=10642495 |doi= |url=http://www.biochemj.org/bj/345/0401/bj3450401.htm]
* By the phosphorylation of diacylglycerol (DAG) by DAG kinase (DAGK)
* By the acylation of lysoPA by lysoPA-acyltransferase (LPAAT); this is the most common pathway:Devlin, T. M. 2004. "Bioquímica", 4ª edición. Reverté, Barcelona. ISBN 84-291-7208-4]

PA is degraded by conversion into DAG by lipid phosphate phosphohydrolases (LPPs)cite journal |author=Brindley DN, Waggoner DW |title=Phosphatidate phosphohydrolase and signal transduction |journal=Chem. Phys. Lipids |volume=80 |issue=1-2 |pages=45–57 |year=1996 |month=May |pmid=8681429 |doi= |url=] cite journal |author=Brindley DN, Waggoner DW |title=Mammalian lipid phosphate phosphohydrolases |journal=J. Biol. Chem. |volume=273 |issue=38 |pages=24281–4 |year=1998 |month=September |pmid=9733709 |doi= |url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=9733709] or into lyso-PA by phospholipase A (PLA).

The role of PA in the cell

The role of PA in the cell can be divided into three categories:
* PA is the precursor for the biosynthesis of many other lipids.
* The physical properties of PA influence membrane curvature.
* PA acts as a signaling lipid, recruiting cytosolic proteins to appropriate membranes (e.g., sphingosine kinase 1cite journal |author=Delon C, Manifava M, Wood E, "et al" |title=Sphingosine kinase 1 is an intracellular effector of phosphatidic acid |journal=J. Biol. Chem. |volume=279 |issue=43 |pages=44763–74 |year=2004 |month=October |pmid=15310762 |doi=10.1074/jbc.M405771200 |url=] ).These three roles are not mutually exclusive. For example, PA may be involved in vesicle formation by promoting membrane curvature and by recruiting the proteins to carry out the much more energetically unfavourable task of neck formation and pinching.

PA as a biosynthetic precursor

PA is a vital cell lipid that acts as a biosynthetic precursor for the formation (directly or indirectly) of all acylglycerol lipids in the cell.cite journal |author=Athenstaedt K, Daum G |title=Phosphatidic acid, a key intermediate in lipid metabolism |journal=Eur. J. Biochem. |volume=266 |issue=1 |pages=1–16 |year=1999 |month=November |pmid=10542045 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1999&volume=266&issue=1&spage=1]

In mammalian and yeast cells, two different pathways are known for the de novo synthesis of PA, the glycerol 3-phosphate pathway or the dihydroxyacetone phosphate pathway. In bacteria, only the former pathway is present, and mutations that block this pathway are lethal, demonstrating the importance of PA. In mammalian and yeast cells, where the enzymes in these pathways are redundant, mutation of any one enzyme is not lethal. However, it is worth noting that "in vitro", the various acyltransferases exhibit different substrate specificities with respect to the acyl-CoAs that are incorporated into PA. Different acyltransferases also have different intracellular distributions, such as the endoplasmic reticulum (ER), the mitochondria or peroxisomes, and local concentrations of activated fatty acids. This suggests that the various acyltransferases present in mammalian and yeast cells may be responsible for producing different pools of PA.cite journal |author=Athenstaedt K, Daum G |title=Phosphatidic acid, a key intermediate in lipid metabolism |journal=Eur. J. Biochem. |volume=266 |issue=1 |pages=1–16 |year=1999 |month=November |pmid=10542045 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1999&volume=266&issue=1&spage=1]

The conversion of PA into diacylglycerol (DAG) by LPPs is the commitment step for the production of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS). In addition, DAG is also converted into CDP-DAG, which is a precursor for phosphatidylglycerol (PG) and phosphoinositides.cite journal |author=Athenstaedt K, Daum G |title=Phosphatidic acid, a key intermediate in lipid metabolism |journal=Eur. J. Biochem. |volume=266 |issue=1 |pages=1–16 |year=1999 |month=November |pmid=10542045 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1999&volume=266&issue=1&spage=1]

PA concentrations are maintained at extremely low levels in the cell by the activity of potent LPPs.cite journal |author=Brindley DN, Waggoner DW |title=Phosphatidate phosphohydrolase and signal transduction |journal=Chem. Phys. Lipids |volume=80 |issue=1-2 |pages=45–57 |year=1996 |month=May |pmid=8681429 |doi= |url=] These convert PA into DAG very rapidly and, because DAG is the precursor for so many other lipids, it too is soon metabolised into other membrane lipids. This means that any upregulation in PA production can be matched, over time, with a corresponding upregulation in LPPs and in DAG metabolising enzymes.

PA is, therefore, essential for lipid synthesis and cell survival, yet, under normal conditions, is maintained at very low levels in the cell.

Biophysical properties of PA

PA is a unique phospholipid in that it has a small highly-charged head group that is very close to the glycerol backbone. PA is known to play roles in both vesicle fissioncite journal |author=Weigert R, Silletta MG, Spanò S, "et al" |title=CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid |journal=Nature |volume=402 |issue=6760 |pages=429–33 |year=1999 |month=November |pmid=10586885 |doi=10.1038/46587 |url=] and fusioncite journal |author=Blackwood RA, Smolen JE, Transue A, "et al" |title=Phospholipase D activity facilitates Ca2+-induced aggregation and fusion of complex liposomes |journal=Am. J. Physiol. |volume=272 |issue=4 Pt 1 |pages=C1279–85 |year=1997 |month=April |pmid=9142853 |doi= |url=http://ajpcell.physiology.org/cgi/pmidlookup?view=reprint&pmid=9142853] , and these roles may relate to the biophysical properties of PA.

At sites of membrane budding or fusion, the membrane becomes or is highly curved. A major event in the budding of vesicles, such as transport carriers from the Golgi, is the creation and subsequent narrowing of the membrane neck. Studies have suggested that this process may be lipid-driven, and have postulated a central role for DAG due to its, likewise, unique molecular shape. The presence of two acyl chains but no headgroup results in a large negative curvature in membranes.cite journal |author=Shemesh T, Luini A, Malhotra V, Burger KN, Kozlov MM |title=Prefission constriction of Golgi tubular carriers driven by local lipid metabolism: a theoretical model |journal=Biophys. J. |volume=85 |issue=6 |pages=3813–27 |year=2003 |month=December |pmid=14645071 |doi= |url=http://www.biophysj.org/cgi/pmidlookup?view=long&pmid=14645071]

The LPAAT BARS-50 has also been implicated in budding from the Golgi.cite journal |author=Weigert R, Silletta MG, Spanò S, "et al" |title=CtBP/BARS induces fission of Golgi membranes by acylating lysophosphatidic acid |journal=Nature |volume=402 |issue=6760 |pages=429–33 |year=1999 |month=November |pmid=10586885 |doi=10.1038/46587 |url=] This suggests that the conversion of lysoPA into PA might affect membrane curvature. LPAAT activity doubles the number of acyl chains, greatly increasing the cross-sectional area of the lipid that lies ‘within’ the membrane while the surface headgroup remains unchanged. This can result in a more negative membrane curvature. Researchers from Utrecht University have looked at the effect of lysoPA versus PA on membrane curvature by measuring the effect these have on the transition temperature of PE from lipid bilayers to nonlamellar phases using ³¹P-NMR.cite journal |author=Kooijman EE, Chupin V, de Kruijff B, Burger KN |title=Modulation of membrane curvature by phosphatidic acid and lysophosphatidic acid |journal=Traffic |volume=4 |issue=3 |pages=162–74 |year=2003 |month=March |pmid=12656989 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=1398-9219&date=2003&volume=4&issue=3&spage=162] The curvature induced by these lipids was shown to be dependent not only on the structure of lsyoPA versus PA but also on dynamic properties, such as the hydration of head groups and inter- and intramolecular interactions. For instance, Ca²⁺ may interact with two PAs to form a neutral but highly-curved complex. The neutralisation of the otherwise repulsive charges of the headgroups and the absence of any stearic hindrance enables strong intermolecular interactions between the acyl chains, resulting in PA-rich microdomains. Thus "in vitro", physiological changes in pH, temperature, and cation concentrations have strong effects on the membrane curvature induced by PA and lysoPA.cite journal |author=Kooijman EE, Chupin V, de Kruijff B, Burger KN |title=Modulation of membrane curvature by phosphatidic acid and lysophosphatidic acid |journal=Traffic |volume=4 |issue=3 |pages=162–74 |year=2003 |month=March |pmid=12656989 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=1398-9219&date=2003&volume=4&issue=3&spage=162] The interconversion of lysoPA, PA, and DAG - and changes in pH and cation concentration - can cause membrane bending and destabilisation, playing a direct role in membrane fission simply by virtue of their biophysical properties. However, though PA and lysoPA have been shown to affect membrane curvature "in vitro"; their role "in vivo" is unclear.

The roles of lysoPA, PA, and DAG in promoting membrane curvature do not preclude a role in recruiting proteins to the membrane. For instance, the Ca²⁺ requirement for the fusion of complex liposomes is not greatly affected by the addition of annexin I, though it is reduced by PLD. However, with annexin I "and" PLD, the extent of fusion is greatly enhanced, and the Ca²⁺ requirement is reduced almost 1000-fold to near physiological levels.cite journal |author=Blackwood RA, Smolen JE, Transue A, "et al" |title=Phospholipase D activity facilitates Ca2+-induced aggregation and fusion of complex liposomes |journal=Am. J. Physiol. |volume=272 |issue=4 Pt 1 |pages=C1279–85 |year=1997 |month=April |pmid=9142853 |doi= |url=http://ajpcell.physiology.org/cgi/pmidlookup?view=reprint&pmid=9142853]

Thus the metabolic, biophysical, recruitment, and signaling roles of PA may be interrelated.

Measurement of PA production

As PA is rapidly converted to DAG, it is very short-lived in the cell. This means that it is difficult to measure PA production and therefore to study the role of PA in the cell. However, PLD activity can be measured by the addition of primary alcohols to the cell. cite journal |author=Morris AJ, Frohman MA, Engebrecht J |title=Measurement of phospholipase D activity |journal=Anal. Biochem. |volume=252 |issue=1 |pages=1–9 |year=1997 |month=October |pmid=9324933 |doi=10.1006/abio.1997.2299 |url=] PLD then carries out a transphosphatidylation reaction, instead of hydrolysis, producing phosphatidyl alcohols in place of PA. The phosphatidyl alcohols are metabolic dead-ends, and can be readily extracted and measured. Thus PLD activity and PA production (if not PA itself) can be measured, and, by blocking the formation of PA, the involvement of PA in cellular processes can be inferred.

PA as a signalling lipid

As described above, PLD hydrolyses PC to form PA and choline. Because choline is very abundant in the cell, PLD activity does not significantly affect choline levels; and choline is unlikely to play any role in signaling.

The role of PLD activation in numerous signaling contexts, combined with the lack of a role for choline, suggests that PA is important in signaling. However, PA is rapidly converted to DAG, and DAG is also known to be a signaling molecule. This raises the question as to whether PA has any direct role in signaling or whether it simply acts as a precursor for DAG production.cite journal |author=Hodgkin MN, Pettitt TR, Martin A, Michell RH, Pemberton AJ, Wakelam MJ |title=Diacylglycerols and phosphatidates: which molecular species are intracellular messengers? |journal=Trends Biochem. Sci. |volume=23 |issue=6 |pages=200–4 |year=1998 |month=June |pmid=9644971 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/S0968-0004(98)01200-6] cite journal |author=Wakelam MJ |title=Diacylglycerol--when is it an intracellular messenger? |journal=Biochim. Biophys. Acta |volume=1436 |issue=1-2 |pages=117–26 |year=1998 |month=December |pmid=9838074 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/S0005-2760(98)00123-4] If it is found that PA acts only as a DAG precursor, then one can raise the question as to why cells should produce DAG using two enzymes when they contain the PLC that could produce DAG in a single step.

PA produced by PLD or by DAGK can be distinguished by the addition of [γ-³²P] ATP. This will show whether the phosphate group is newly derived from the kinase activity or whether it originates from the PC.cite journal |author=Cockcroft S, Baldwin JM, Allan D |title=The Ca2+-activated polyphosphoinositide phosphodiesterase of human and rabbit neutrophil membranes |journal=Biochem. J. |volume=221 |issue=2 |pages=477–82 |year=1984 |month=July |pmid=6089740 |doi= |url=]

Although PA and DAG are interconvertible, they do not act in the same pathways. Stimuli that activate PLD do not activate enzymes downstream of DAG, and vice versa. For example it was shown that addition of PLD to membranes results in the production of [³²P] -labeled PA and [³²P] -labeled phosphoinositides.cite journal |author=Moritz A, De Graan PN, Gispen WH, Wirtz KW |title=Phosphatidic acid is a specific activator of phosphatidylinositol-4-phosphate kinase |journal=J. Biol. Chem. |volume=267 |issue=11 |pages=7207–10 |year=1992 |month=April |pmid=1313792 |doi= |url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=1313792] The addition of DAGK inhibitors eliminates the production of [³²P] -labeled PA but not the PLD-stimulated production of phosphoinositides.

It is possible that, though PA and DAG are interconvertible, separate pools of signaling and non-signaling lipids may be maintained. Studies have suggested that DAG signaling is mediated by polyunsaturated DAG, whereas PLD-derived PA is monounsaturated or saturated. Thus functional saturated/monounsaturated PA can be degraded by hydrolysing it to form non-functional saturated/monounsaturated DAG, whereas functional polyunsaturated DAG can be degraded by converting it into non-functional polyunsaturated PA.cite journal |author=Bocckino SB, Blackmore PF, Wilson PB, Exton JH |title=Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism |journal=J. Biol. Chem. |volume=262 |issue=31 |pages=15309–15 |year=1987 |month=November |pmid=3117799 |doi= |url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=3117799] cite journal |author=Hodgkin MN, Pettitt TR, Martin A, Michell RH, Pemberton AJ, Wakelam MJ |title=Diacylglycerols and phosphatidates: which molecular species are intracellular messengers? |journal=Trends Biochem. Sci. |volume=23 |issue=6 |pages=200–4 |year=1998 |month=June |pmid=9644971 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/S0968-0004(98)01200-6]

This model suggests that PA and DAG effectors should be able to distinguish lipids with the same headgroups but with differing acyl chains. Although some lipid-binding proteins are able to insert themselves into membranes and could hypothetically recognise the type of acyl chain or the resulting properties of the membrane, many lipid-binding proteins are cytosolic and localise to the membrane by binding only the headgroups of lipids. Perhaps the different acyl chains can affect the angle of the head-group in the membrane. If this is the case, it suggests that a PA-binding domain must not only be able to bind PA specifically but must also be able to identify those head-groups that are at the correct angle. Whatever the mechanism is, such specificity is possible. It is seen in the pig testes DAGK that is specific for polyunsaturated DAGcite journal |author=Hodgkin MN, Gardner SD, Rose S, Paterson A, Martin A, Wakelam MJ |title=Purification and characterization of sn-1-stearoyl-2-arachidonoylglycerol kinase from pig testes |journal=Biochem. J. |volume=322 ( Pt 2) |issue= |pages=529–34 |year=1997 |month=March |pmid=9065773 |doi= |url=http://www.biochemj.org/bj/322/0529/bj3220529.htm] and in two rat hepatocyte LPPs that dephosphorylate different PA species with different activities.cite journal |author=Fleming IN, Yeaman SJ |title=Purification and characterization of N-ethylmaleimide-insensitive phosphatidic acid phosphohydrolase (PAP2) from rat liver |journal=Biochem. J. |volume=308 ( Pt 3) |issue= |pages=983–9 |year=1995 |month=June |pmid=8948459 |doi= |url=] Moreover, the stimulation of SK1 activity by PS "in vitro" was shown to vary greatly depending on whether dioleoyl (C18:1), distearoyl (C18:0), or 1-stearoyl, 2-oleoyl species of PS were used.cite journal |author=Olivera A, Rosenthal J, Spiegel S |title=Effect of acidic phospholipids on sphingosine kinase |journal=J. Cell. Biochem. |volume=60 |issue=4 |pages=529–37 |year=1996 |month=March |pmid=8707892 |doi=10.1002/(SICI)1097-4644(19960315)60:4<529::AID-JCB9>3.0.CO;2-U |url= |doi_brokendate=2008-06-22] Thus it seems that, though PA and DAG are interconvertible, the different species of lipid can have different biological activities; and this may enable the two lipids to maintain separate signaling pathways.

Proteins known to interact with PA

*SK1
*PDE4A1
*Raf1
*mTOR
*PP1
*SHP1
*Spo20p
*p47phox
*PKCε
* PLCβ
*PIP5K.

References

External links