- MicA RNA
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SraD RNA 
Predicted secondary structure and sequence conservation of SraD Identifiers Symbol SraD Alt. Symbols sraD Rfam RF00078 Other data RNA type Gene; sRNA Domain(s) Bacteria SO 0000655 The MicA RNA (also known as SraD) is a small non-coding RNA that was discovered in E. coli during a large scale screen.[1] Expression of SraD is highly abundant in stationary phase, but low levels could be detected in exponentially growing cells as well.
Contents
Function
This RNA binds the Hfq protein and regulates levels of gene expression by an antisense mechanism. It is known to target the OmpA gene in E. coli and occludes the ribosome binding site.[2] Under conditions of envelope stress, micA transcription is induced. MicA and RybB RNA transcription is under the control of the sigma factor sigma(E).[3][4]
The PhoPQ two-component system is repressed by MicA. The RNA presumably pairs with the ribosomal binding site of phoP mRNA, thereby inhibiting translation. This links micA to cellular processes such as Mg(2+) transport, virulence, LPS modifications and resistance to antimicrobial peptides.[5]
In S. typhimurium MicA has been shown to be involved in biofilm formation.[6]
References
- ^ Argaman, L; Hershberg R, Vogel J, Bejerano G, Wagner EG, Margalit H, Altuvia S (2001). "Novel small RNA-encoding genes in the intergenic regions of Escherichia coli". Curr Biol 11 (12): 941–950. doi:10.1016/S0960-9822(01)00270-6. PMID 11448770.
- ^ Udekwu KI, Darfeuille F, Vogel J, Reimegård J, Holmqvist E, Wagner EG (2005). "Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA". Genes Dev. 19 (19): 2355–66. doi:10.1101/gad.354405. PMC 1240044. PMID 16204185. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1240044.
- ^ Udekwu KI, Wagner EG (2007). "Sigma E controls biogenesis of the antisense RNA MicA". Nucleic Acids Res. 35 (4): 1279–88. doi:10.1093/nar/gkl1154. PMC 1851643. PMID 17267407. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1851643.
- ^ Johansen J, Rasmussen AA, Overgaard M, Valentin-Hansen P (2006). "Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins". J. Mol. Biol. 364 (1): 1–8. doi:10.1016/j.jmb.2006.09.004. PMID 17007876.
- ^ Coornaert A, Lu A, Mandin P, Springer M, Gottesman S, Guillier M (2010). "MicA sRNA links the PhoP regulon to cell envelope stress.". Mol Microbiol 76 (2): 467–79. doi:10.1111/j.1365-2958.2010.07115.x. PMC 2925231. PMID 20345657. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2925231.
- ^ Kint G, De Coster D, Marchal K, Vanderleyden J, De Keersmaecker SC (2010). "The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation". BMC Microbiol. 10: 276. doi:10.1186/1471-2180-10-276. PMC 2987988. PMID 21044338. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2987988.
Further reading
- Karavolos MH, Bulmer DM, Spencer H, Rampioni G, Schmalen I, Baker S, Pickard D, Gray J, Fookes M, Winzer K, Ivens A, Dougan G, Williams P, Anjam Khan CM (2011). "Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E.". EMBO Rep 12 (3): 252–8. doi:10.1038/embor.2011.4. PMID 21331094.
External links
Categories:- Non-coding RNA
- Molecular and cellular biology stubs
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