MicX sRNA

MicX Vibrio cholerae sRNA
Secondary structure of MicX sRNA.
Identifiers
Symbol	MicX
Rfam	RF01808
Other data
RNA type	sRNA
Domain(s)	Vibrio cholerae

MicX sRNA (formerly known as A10)^[1] is a small non-coding RNA found in Vibrio cholerae.^[2] It was given the name MicX as it has a similar function to MicA, MicC and MicF in E. coli.^[3] MicX sRNA negatively regulates an outer membrane protein (coded for by VC0972) and also a component of an ABC transporter (gene VC0620).^[4] These interactions were predicted and then confirmed using a DNA microarray.^[2]

MicX was identified through a bioinformatics screen of V. cholerae having been previously predicted.^[1] Levels of transcription of this sRNA were compared under several conditions: it was found to be expressed on all tested mediums; richer mediums slightly reduced transcription; repression of certain sigma factors (δ^S and δ^E) did not change transcription but it was dramatically reduced in the absence of Hfq protein.^[2] This observation is in accordance with other sRNA expression patterns.^[5][6]

The MicX RNA gene overlaps with VCA0943 - a gene coding for a maltose transporter permease - but the ribonuclease RNAase E processes the MicX transcript to create an active and stable form containing only the VCA0943 3' UTR.^[2]

See also

• Hfq protein

References

1. ^ ^{a b} Livny, Jonathan; Fogel, Michael A.; Davis, Brigid M.; Waldor, Matthew K. (2005). "sRNAPredict: an integrative computational approach to identify sRNAs in bacterial genomes". Nucleic Acids Research 33 (13): 4096–105. doi:10.1093/nar/gki715. PMC 1180744. PMID 16049021. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1180744. 
2. ^ ^{a b c d} Davis, Brigid M.; Waldor, Matthew K. (2007). "RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA". Molecular Microbiology 65 (2): 373–85. doi:10.1111/j.1365-2958.2007.05796.x. PMC 1976385. PMID 17590231. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1976385. 
3. ^ Mizuno, T.; Chou, M. Y.; Inouye, M. (1984). "A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)". Proceedings of the National Academy of Sciences 81 (7): 1966–70. doi:10.1073/pnas.81.7.1966. PMC 345417. PMID 6201848. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=345417. 
4. ^ Song, Tianyan; Mika, Franziska; Lindmark, Barbro; Liu, Zhi; Schild, Stefan; Bishop, Anne; Zhu, Jun; Camilli, Andrew et al. (2008). "A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles". Molecular Microbiology 70 (1): 100–11. doi:10.1111/j.1365-2958.2008.06392.x. PMC 2628432. PMID 18681937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2628432. 
5. ^ Sledjeski, Darren D.; Whitman, Christina; Zhang, Aixia (2001). "Hfq Is Necessary for Regulation by the Untranslated RNA DsrA". Journal of Bacteriology 183 (6): 1997–2005. doi:10.1128/JB.183.6.1997-2005.2001. PMC 95095. PMID 11222598. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=95095. 
6. ^ Massé, Eric; Escorcia, Freddy E.; Gottesman, Susan (2003). "Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli". Genes & Development 17 (19): 2374–83. doi:10.1101/gad.1127103. PMC 218075. PMID 12975324. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=218075. 

Further reading

• Gottesman, Susan (2004). "The small RNA regulators of Escherichia coli: roles and mechanisms". Annual Review of Microbiology 58: 303–28. doi:10.1146/annurev.micro.58.030603.123841. PMID 15487940. 
• Schlüter, Jan-Philip; Reinkensmeier, Jan; Daschkey, Svenja; Evguenieva-Hackenberg, Elena; Janssen, Stefan; Jänicke, Sebastian; Becker, Jörg D.; Giegerich, Robert et al. (2010). "A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti". BMC Genomics 11: 245. doi:10.1186/1471-2164-11-245. PMC 2873474. PMID 20398411. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2873474. 
• Sittka, Alexandra; Sharma, Cynthia M.; Rolle, Katarzyna; Vogel, Jörg (July 2009). "Deep sequencing of Salmonella RNA associated with heterologous Hfq proteins in vivo reveals small RNAs as a major target class and identifies RNA processing phenotypes". RNA Biology 6 (3): 266–75. doi:10.4161/rna.6.3.8332. PMID 19333007. 

External links

• Rfam page for MicX Vibrio cholerae sRNA