MicX sRNA

MicX sRNA
MicX Vibrio cholerae sRNA
MicX SScons.png
Secondary structure of MicX sRNA.
Identifiers
Symbol MicX
Rfam RF01808
Other data
RNA type sRNA
Domain(s) Vibrio cholerae

MicX sRNA (formerly known as A10)[1] is a small non-coding RNA found in Vibrio cholerae.[2] It was given the name MicX as it has a similar function to MicA, MicC and MicF in E. coli.[3] MicX sRNA negatively regulates an outer membrane protein (coded for by VC0972) and also a component of an ABC transporter (gene VC0620).[4] These interactions were predicted and then confirmed using a DNA microarray.[2]

MicX was identified through a bioinformatics screen of V. cholerae having been previously predicted.[1] Levels of transcription of this sRNA were compared under several conditions: it was found to be expressed on all tested mediums; richer mediums slightly reduced transcription; repression of certain sigma factors (δS and δE) did not change transcription but it was dramatically reduced in the absence of Hfq protein.[2] This observation is in accordance with other sRNA expression patterns.[5][6]

The MicX RNA gene overlaps with VCA0943 - a gene coding for a maltose transporter permease - but the ribonuclease RNAase E processes the MicX transcript to create an active and stable form containing only the VCA0943 3' UTR.[2]

Contents

See also

References

  1. ^ a b Livny, Jonathan; Fogel, Michael A.; Davis, Brigid M.; Waldor, Matthew K. (2005). "sRNAPredict: an integrative computational approach to identify sRNAs in bacterial genomes". Nucleic Acids Research 33 (13): 4096–105. doi:10.1093/nar/gki715. PMC 1180744. PMID 16049021. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1180744. 
  2. ^ a b c d Davis, Brigid M.; Waldor, Matthew K. (2007). "RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA". Molecular Microbiology 65 (2): 373–85. doi:10.1111/j.1365-2958.2007.05796.x. PMC 1976385. PMID 17590231. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1976385. 
  3. ^ Mizuno, T.; Chou, M. Y.; Inouye, M. (1984). "A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)". Proceedings of the National Academy of Sciences 81 (7): 1966–70. doi:10.1073/pnas.81.7.1966. PMC 345417. PMID 6201848. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=345417. 
  4. ^ Song, Tianyan; Mika, Franziska; Lindmark, Barbro; Liu, Zhi; Schild, Stefan; Bishop, Anne; Zhu, Jun; Camilli, Andrew et al. (2008). "A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles". Molecular Microbiology 70 (1): 100–11. doi:10.1111/j.1365-2958.2008.06392.x. PMC 2628432. PMID 18681937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2628432. 
  5. ^ Sledjeski, Darren D.; Whitman, Christina; Zhang, Aixia (2001). "Hfq Is Necessary for Regulation by the Untranslated RNA DsrA". Journal of Bacteriology 183 (6): 1997–2005. doi:10.1128/JB.183.6.1997-2005.2001. PMC 95095. PMID 11222598. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=95095. 
  6. ^ Massé, Eric; Escorcia, Freddy E.; Gottesman, Susan (2003). "Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli". Genes & Development 17 (19): 2374–83. doi:10.1101/gad.1127103. PMC 218075. PMID 12975324. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=218075. 

Further reading

External links


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