CNKSR1

Connector enhancer of kinase suppressor of Ras 1
PDB rendering based on 1wwv.
Available structures PDB 1wwv
Identifiers
Symbols	CNKSR1; CNK; CNK1; KSR
External IDs	OMIM: 603272 MGI: 2670958 HomoloGene: 4604 GeneCards: CNKSR1 Gene
Gene Ontology Molecular function • protein binding • protein binding, bridging Cellular component • cytoplasm • plasma membrane • cell-cell junction • cell cortex Biological process • transmembrane receptor protein tyrosine kinase signaling pathway • Ras protein signal transduction • Rho protein signal transduction Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10256	194231
Ensembl	ENSG00000142675	ENSMUSG00000028841
UniProt	Q969H4	n/a
RefSeq (mRNA)	NM_006314.2	NM_001081047.1
RefSeq (protein)	NP_006305	NP_001074516.1
Location (UCSC)	Chr 1: 26.5 – 26.52 Mb	Chr 4: 133.78 – 133.79 Mb
PubMed search	[1]	[2]

Connector enhancer of kinase suppressor of ras 1 is an enzyme that in humans is encoded by the CNKSR1 gene.^[1][2]

This gene is a necessary element in receptor tyrosine kinase pathways, possibly as a tyrosine phosphorylation target. It is involved in regulation of RAF in the MAPK pathway and may also play a role in a MAPK-independent pathway.^[2]

Interactions

CNKSR1 has been shown to interact with RhoD^[3] and RASSF1.^[4]

References

1. ^ Therrien M, Wong AM, Rubin GM (Dec 1998). "CNK, a RAF-binding multidomain protein required for RAS signaling". Cell 95 (3): 343–53. doi:10.1016/S0092-8674(00)81766-3. PMID 9814705. 
2. ^ ^{a b} "Entrez Gene: CNKSR1 connector enhancer of kinase suppressor of Ras 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10256. 
3. ^ Jaffe, Aron B; Aspenström Pontus, Hall Alan (Feb. 2004). "Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways". Mol. Cell. Biol. (United States) 24 (4): 1736–46. doi:10.1128/MCB.24.4.1736-1746.2004. ISSN 0270-7306. PMC 344169. PMID 14749388. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=344169. 
4. ^ Rabizadeh, Shahrooz; Xavier Ramnik J, Ishiguro Kazuhiro, Bernabeortiz Juliocesar, Lopez-Ilasaca Marco, Khokhlatchev Andrei, Mollahan Pamela, Pfeifer Gerd P, Avruch Joseph, Seed Brian (Jul. 2004). "The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death". J. Biol. Chem. (United States) 279 (28): 29247–54. doi:10.1074/jbc.M401699200. ISSN 0021-9258. PMID 15075335. 

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Therrien M, Wong AM, Kwan E, Rubin GM (1999). "Functional analysis of CNK in RAS signaling". Proc. Natl. Acad. Sci. U.S.A. 96 (23): 13259–63. doi:10.1073/pnas.96.23.13259. PMC 23935. PMID 10557308. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=23935. 
• Hartsough MT, Morrison DK, Salerno M et al. (2002). "Nm23-H1 metastasis suppressor phosphorylation of kinase suppressor of Ras via a histidine protein kinase pathway". J. Biol. Chem. 277 (35): 32389–99. doi:10.1074/jbc.M203115200. PMID 12105213. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Matheny SA, Chen C, Kortum RL et al. (2004). "Ras regulates assembly of mitogenic signalling complexes through the effector protein IMP". Nature 427 (6971): 256–60. doi:10.1038/nature02237. PMID 14724641. 
• Jaffe AB, Aspenström P, Hall A (2004). "Human CNK1 acts as a scaffold protein, linking Rho and Ras signal transduction pathways". Mol. Cell. Biol. 24 (4): 1736–46. doi:10.1128/MCB.24.4.1736-1746.2004. PMC 344169. PMID 14749388. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=344169. 
• Rabizadeh S, Xavier RJ, Ishiguro K et al. (2004). "The scaffold protein CNK1 interacts with the tumor suppressor RASSF1A and augments RASSF1A-induced cell death". J. Biol. Chem. 279 (28): 29247–54. doi:10.1074/jbc.M401699200. PMID 15075335. 
• Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Jaffe AB, Hall A, Schmidt A (2005). "Association of CNK1 with Rho guanine nucleotide exchange factors controls signaling specificity downstream of Rho". Curr. Biol. 15 (5): 405–12. doi:10.1016/j.cub.2004.12.082. PMID 15753034. 
• Ziogas A, Moelling K, Radziwill G (2005). "CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation". J. Biol. Chem. 280 (25): 24205–11. doi:10.1074/jbc.M413327200. PMID 15845549. 
• Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
• Fritz RD, Radziwill G (2006). "The scaffold protein CNK1 interacts with the angiotensin II type 2 receptor". Biochem. Biophys. Res. Commun. 338 (4): 1906–12. doi:10.1016/j.bbrc.2005.10.168. PMID 16289034. 
• Beausoleil SA, Villén J, Gerber SA et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.