NT5C3

5'-nucleotidase, cytosolic III
PDB rendering based on 2bdu.
Available structures PDB 2CN1, 2JGA, 2VKQ
Identifiers
Symbols	NT5C3; MGC27337; MGC87109; MGC87828; P5'N-1; PN-I; PSN1; UMPH; UMPH1; cN-III
External IDs	OMIM: 606224 MGI: 1927186 HomoloGene: 9534 GeneCards: NT5C3 Gene
EC number	3.1.3.5
Gene Ontology Molecular function • nucleotide binding • magnesium ion binding • 5'-nucleotidase activity • 5'-nucleotidase activity • 5'-nucleotidase activity • 2'-phosphotransferase activity • 2'-phosphotransferase activity • hydrolase activity Cellular component • cytoplasm • mitochondrion • endoplasmic reticulum • cytosol Biological process • pyrimidine base metabolic process • pyrimidine nucleoside metabolic process • nucleotide metabolic process • pyrimidine nucleoside catabolic process • nucleobase, nucleoside and nucleotide metabolic process Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	51251	107569
Ensembl	ENSG00000122643	ENSMUSG00000029780
UniProt	Q9H0P0	Q9D020
RefSeq (mRNA)	NM_001002010.1	NM_026004.3
RefSeq (protein)	NP_001002010.1	NP_080280.3
Location (UCSC)	Chr 7: 33.05 – 33.1 Mb	Chr 6: 56.83 – 56.87 Mb
PubMed search	[1]	[2]

Cytosolic 5'-nucleotidase 3 is an enzyme that in humans is encoded by the NT5C3 gene.^[1][2][3]

Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.[supplied by OMIM]^[3]

References

1. ^ Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (Nov 2000). "Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells". Genome Res 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310934. 
2. ^ Amici A, Emanuelli M, Raffaelli N, Ruggieri S, Saccucci F, Magni G (Sep 2000). "Human erythrocyte pyrimidine 5-nucleotidase, PN-I, is identical to p36, a protein associated to lupus inclusion formation in response to alpha-interferon". Blood 96 (4): 1596–8. PMID 10942414. 
3. ^ ^{a b} "Entrez Gene: NT5C3 5'-nucleotidase, cytosolic III". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51251. 

Further reading

• Amici A, Emanuelli M, Ferretti E, et al. (1995). "Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties". Biochem. J. 304 ( Pt 3) (Pt 3): 987–92. PMC 1137429. PMID 7818506. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1137429. 
• Rich SA, Bose M, Tempst P, Rudofsky UH (1996). "Purification, microsequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions". J. Biol. Chem. 271 (2): 1118–26. doi:10.1074/jbc.271.2.1118. PMID 8557639. 
• Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549. 
• Amici A, Emanuelli M, Magni G, et al. (1998). "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides". FEBS Lett. 419 (2–3): 263–7. doi:10.1016/S0014-5793(97)01464-6. PMID 9428647. 
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948. 
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072. 
• Marinaki AM, Escuredo E, Duley JA, et al. (2001). "Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency". Blood 97 (11): 3327–32. doi:10.1182/blood.V97.11.3327. PMID 11369620. 
• Amici A, Magni G (2002). "Human erythrocyte pyrimidine 5'-nucleotidase, PN-I". Arch. Biochem. Biophys. 397 (2): 184–90. doi:10.1006/abbi.2001.2676. PMID 11795870. 
• Amici A, Emanuelli M, Ruggieri S, et al. (2003). "Kinetic evidence for covalent phosphoryl-enzyme intermediate in phosphotransferase activity of human red cell pyrimidine nucleotidases". Meth. Enzymol.. Methods in Enzymology 354: 149–59. doi:10.1016/S0076-6879(02)54011-8. ISBN 9780121822576. PMID 12418222. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Balta G, Gumruk F, Akarsu N, et al. (2003). "Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency". Blood 102 (5): 1900–3. doi:10.1182/blood-2003-02-0628. PMID 12714505. 
• Bianchi P, Fermo E, Alfinito F, et al. (2003). "Molecular characterization of six unrelated Italian patients affected by pyrimidine 5'-nucleotidase deficiency". Br. J. Haematol. 122 (5): 847–51. doi:10.1046/j.1365-2141.2003.04532.x. PMID 12930399. 
• Kanno H, Takizawa T, Miwa S, Fujii H (2004). "Molecular basis of Japanese variants of pyrimidine 5'-nucleotidase deficiency". Br. J. Haematol. 126 (2): 265–71. doi:10.1111/j.1365-2141.2004.05029.x. PMID 15238149. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930. 
• Chiarelli LR, Bianchi P, Fermo E, et al. (2005). "Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia". Blood 105 (8): 3340–5. doi:10.1182/blood-2004-10-3895. PMID 15604219. 
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347501.