Creatininase

Creatininase
Crystallographic structure of a creatinine amidohydrolase from Nostoc pruniforme.[1]
Identifiers
EC number	3.5.2.10
CAS number	9025-13-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

Creatininase
creatininase-product complex
Identifiers
Symbol	Creatininase
Pfam	PF02633
InterPro	IPR003785
SCOP	1v7z
Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary

In enzymology, a creatininase (EC 3.5.2.10) is an enzyme that catalyses the hydrolysis of creatinine to creatine, which can then be metabolised to urea and sarcosine by creatinase.

creatinine + H₂O $\rightleftharpoons$ creatine

Thus, the two substrates of this enzyme are creatinine and H₂O, whereas its product is creatine.

Creatininase is a member of the urease-related amidohydrolases ^[2], the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is creatinine amidohydrolase. This enzyme is also called creatinine hydrolase. This enzyme participates in arginine and proline metabolism.

Structural studies

Creatininase from Pseudomonas putida has a core structure consisting of 3-layers, alpha/beta/alpha.^[3]

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1J2T, 1J2U, 1Q3K, and 1V7Z.

References

1. ^ PDB 3NO4; Joint Center for Structural Genomics (2010). Crystal structure of a creatinine amidohydrolase (Npun_F1913) from Nostoc punctiforme PCC 73102 at 2.00 A resolution. doi:10.2210/pdb3no4/pdb. 
2. ^ Yamamoto K, Oka M, Kikuchi T, Emi S (1995). "Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7". Biosci. Biotechnol. Biochem. 59 (7): 1331–1332. doi:10.1271/bbb.59.1331. PMID 7670196. 
3. ^ Yoshimoto T, Tanaka N, Kanada N, Inoue T, Nakajima Y, Haratake M, Nakamura KT, Xu Y, Ito K (March 2004). "Crystal structures of creatininase reveal the substrate binding site and provide an insight into the catalytic mechanism". J. Mol. Biol. 337 (2): 399–416. doi:10.1016/j.jmb.2004.01.022. PMID 15003455. 

• Tsuru, D, Oka I and Yoshimoto T (1976). "Creatinine decomposing enzymes in Pseudomonas putida". Agric. Biol. Chem. 40: 1011–1018. 

This hydrolase article is a stub. You can help Wikipedia by expanding it.v · Categories: Hydrolase stubs EC 3.5.2 Enzymes of known structure Wikimedia Foundation. 2010. Игры ⚽ Поможем написать реферат Gaston Bonnier Mauritania Airways Look at other dictionaries: creatininase — An amidohydrolase catalyzing the conversion of creatine to creatinine. * * * cre·a·ti·ni·nase (kre atґĭ nin ās) [EC 3.5.2.10] an amidohydrolase that catalyzes the conversion of creatinine to creatine …   Medical dictionary List of EC numbers (EC 3) — This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.EC 3.1: Acting on Ester BondsEC… …   Wikipedia Hydrolase — Cristal de lysozyme Les hydrolases constituent une classe d enzymes qui catalysent les réactions d hydrolyse de molécules suivant la réaction générale : R R + H2O ⇌ R OH + R H On y trouve par exemple les estérases, qui hydrolysent les …   Wikipédia en Français EC 3.5 — Le groupe EC3.5 rassemble des enzymes appartenant à la famille des hydrolases. Elles ont pour particularité de catalyser des réactions d hydrolyse dans des molécules possédant un carbone et un azote liés par liaison simple, double ou triple. À la …   Wikipédia en Français 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”