- PAS diastase
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PAS diastase is a histological stain often used by pathologists as an ancillary study in making a diagnosis on paraffin-embedded tissue samples. Diastase is an enzyme that breaks down glycogen and PAS is a stain that typically gives a magenta color in the presence of glycogen. When the two are used together a light pink color replaces the deep magenta. Differences in the intensities of the two stains (PAS and PAS-D) can be attributed to different glycogen concentrations and can be used to semiquantify glycogen in samples. In practice, the tissue is deparaffinized, the diastase incubates, and then the PAS stain is applied.
Examples
In histology, PAS diastase is a stain used to show gastric duodenal metaplasia in duodenal adenomas.[1]
Contents
Additional images
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Histoplasma in a granuloma. PAS diastase stain.
See also
References
- ^ Rubio CA (2007). "Gastric duodenal metaplasia in duodenal adenomas". J. Clin. Pathol. 60 (6): 661–3. doi:10.1136/jcp.2006.039388. PMC 1955048. PMID 16837629. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1955048.
External links
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