Multifocal multiphoton microscopy

Multifocal multiphoton microscopy

Multifocal multiphoton microscopy is a microscopy technique for generating 3D images. By exploiting specific properties of pulsed-mode multiphoton excitation the conflict between the density of the foci, i.e. the degree of parallelization, and the axial sectioning has been resolved.

The basic idea is that the laser pulses of neighboring foci are temporally separated by at least one pulse duration, so that interference is avoided. This method is referred to as time-multiplexing (TMX). Moreover, with a high degree of time multiplicity, the interfocal distance can be reduced to such an extent that lateral scanning becomes obsolete. In this case axial scanning is sufficient to record a 3D-image.

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v · Microscope · Optical microscopy
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Fluorescence methods
Sub-diffraction
limit techniques
Diffraction limit · Stimulated emission depletion (STED) · Photo-activated localization microscopy (PALM) · Near-field (NSOM/SNOM)



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  • Multifocal Multiphoton Microscopy — By exploiting specific properties of pulsed mode multiphoton excitation the conflict between the density of the foci, i.e. the degree of parallelization, and the axial sectioning has been resolved. The basic idea is that the laser pulses of… …   Wikipedia

  • MMM — may have one of the following meanings: The number 3000, in Roman numerals Marketing mix modeling, a method of statistical analysis The Master of Management and Manufacturing program, a 2 year graduate level dual degree MBA + MEM program offered… …   Wikipedia

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