Minimum Information About a Proteomics Experiment

MIAPE (Minimum Information About a Proteomics Experiment) is a minimum information standard created by the HUPO Proteomics Standards Initiative for reporting proteomics experiments^[1]. It is intended to specify all the information necessary to interpret the results of the experiment unambiguously and to potentially reproduce the experiment. While the standard defines the content required for compliant reports, it does not specify the format in which this data should be presented (which is left to the corresponding *ML format, also defined by PSI^[2]), nor does it define how to perform experiments^[3].

Working groups

Several working groups work on several documents covering the different areas of proteomics^[4]:

Gel electrophoresis

The Gel electrophoresis working group defined reporting requirements for Gel electrophoresis experiments. The document is at the stage of a recommandation and has been published.^[5] The corresponding data exchange format is called GelML and a stable version was released in late 2007.^[6]

The Gel electrophoresis working group also focuses on image analysis with the Gel image informatics recommendation that is currently in public review phase while the corresponding exchange format is only a draft (as of April 2009).^[6]

Sample Processing

The Sample Processing working group defines requirements concerning all the sample pre-processing steps that are carried out before Gel electrophoresis or Mass spectrometry is applied. Two documents concerning Column chromatography and Capillary electrophoresis are in the early draft stages and the Sample preparation and handling is still a project (as of April 2009). The data exchange format (spML) is also under development.^[7]

Mass Spectrometry

Both Mass spectrometry^[8] and Mass spectrometry informatics^[9] documents have been published as recommandations by the Mass Spectrometry working group.

The working group has released several data exchange format: the mzML, which is a merge of the previous competitors mzData (developed by PSI) and mzXML (developed at the Seattle Proteome Center at the Institute for Systems Biology); AnalysisXML, for Mass spectra informatics analysis; and TraML, for Selected reaction monitoring. Finally they develop MS CV, a controlled vocabulary to use with the previous file formats.^[10]

Molecular Interactions

The Molecular interactions working group of PSI only works on PSI MI XML, a data exchange format, and on its corresponding ontologies. They have published the MIMIx guidelines (Minimum Information about a Molecular Interaction experiment)

General

Study design and sample generation and Statistical analysis of data MIAPE recommendations are also being planned or drafted^[4].

Standard-compliant proteomics repositories

Several standard-compliant proteomics repositories exist, allowing researchers to publish their data while enforcing MIAPE guidelines. For example:

• MIAPEGelDB^[11] (for gel electrophoresis data)
• PRIDE^[12] (for mass spectrometry data)
• ProteoRed MIAPE Generator tool^[13] (for gel electrophoresis and mass spectrometry data)

It is expected that journal editors will eventually request authors to publish all their data to such repositories before publication^{[citation needed]}.

Similar initiatives

There are similar initiatives that try to define minimal requirements. For microarrays the MGED Society defined the Minimum Information About a Microarray Experiment (MIAME).^[14] The Standards for Reporting of Diagnostic Accuracy (STARD) is available for studies reporting medical diagnosis accuracies.^[15]

References

1. ^ "MIAPE home page". HUPO Proteomics Standards Initiative. http://www.psidev.info/index.php?q=node/91. Retrieved 2008-12-05. 
2. ^ Hermjakob, H (2006). "The HUPO Proteomics Standards Initiative - Overcoming the fragmentation of Proteomics Data". Practical Proteomics 6 (S2): 34–38. doi:10.1002/pmic.200600537. PMID 17031794. 
3. ^ Taylor, Chris (2006). "Minimum Reporting Requirements for Proteomics: A MIAPE Primer". Practical Proteomics 6 (S2): 39–44. doi:10.1002/pmic.200600549. PMID 17031795. 
4. ^ ^{a b} "MIAPE home page". HUPO Proteomics Standards Initiative. http://www.psidev.info/. Retrieved 2009-04-23. 
5. ^ Gibson, Frank; Leigh Anderson, Gyorgy Babnigg, Mark Baker, Matthias Berth, Pierre-Alain Binz, Andy Borthwick, Phil Cash, Billy W Day, David B Friedman, Donita Garland, Howard B Gutstein, Christine Hoogland, Neil A Jones, Alamgir Khan, Joachim Klose, Angus I Lamond, Peter F Lemkin, Kathryn S Lilley, Jonathan Minden, Nicholas J Morris, Norman W Paton, Michael R Pisano, John E Prime, Thierry Rabilloud, David A Stead, Chris F Taylor, Hans Voshol, Anil Wipat, Andrew R Jones (2008). "Guidelines for reporting the use of gel electrophoresis in proteomics". Nat Biotech 26 (8): 863–864. doi:10.1038/nbt0808-863. ISSN 1087-0156. PMID 18688234. 
6. ^ ^{a b} "MIAPE Gel Electrophoresis working group page". HUPO Proteomics Standards Initiative. http://psidev.info/index.php?q=node/83. Retrieved 2009-04-23. 
7. ^ "MIAPE Sample Processing working group page". HUPO Proteomics Standards Initiative. http://psidev.info/index.php?q=node/90. Retrieved 2009-04-23. 
8. ^ Taylor, Chris F; Pierre-Alain Binz, Ruedi Aebersold, Michel Affolter, Robert Barkovich, Eric W Deutsch, David M Horn, Andreas Huhmer, Martin Kussmann, Kathryn Lilley, Marcus Macht, Matthias Mann, Dieter Muller, Thomas A Neubert, Janice Nickson, Scott D Patterson, Roberto Raso, Kathryn Resing, Sean L Seymour, Akira Tsugita, Ioannis Xenarios, Rong Zeng, Randall K Julian (2008). "Guidelines for reporting the use of mass spectrometry in proteomics". Nat Biotech 26 (8): 860–861. doi:10.1038/nbt0808-860. ISSN 1087-0156. PMID 18688232. 
9. ^ Binz, Pierre-Alain; Robert Barkovich, Ronald C Beavis, David Creasy, David M Horn, Randall K Julian, Sean L Seymour, Chris F Taylor, Yves Vandenbrouck (2008). "Guidelines for reporting the use of mass spectrometry informatics in proteomics". Nat Biotech 26 (8): 862. doi:10.1038/nbt0808-862. ISSN 1087-0156. PMID 18688233. 
10. ^ "MIAPE Mass Spectrometry working group page". HUPO Proteomics Standards Initiative. http://psidev.info/index.php?q=wiki/Mass_Spectrometry. Retrieved 2009-04-23. 
11. ^ "MIAPEGelDB home page". ExPASy. http://miapegeldb.expasy.org/. Retrieved 2008-12-07. 
12. ^ "PRIDE PRoteomics IDEntifications database home page". European Bioinformatics Institute. http://www.ebi.ac.uk/pride/. Retrieved 2008-12-07. 
13. ^ "MIAPE Generator tool". ProteoRed. http://www.proteored.org/MIAPEtool.asp. Retrieved 2009-03-06. 
14. ^ Brazma, Alvis; Pascal Hingamp, John Quackenbush, Gavin Sherlock, Paul Spellman, Chris Stoeckert, John Aach, Wilhelm Ansorge, Catherine A. Ball, Helen C. Causton, Terry Gaasterland, Patrick Glenisson, Frank C.P. Holstege, Irene F. Kim, Victor Markowitz, John C. Matese, Helen Parkinson, Alan Robinson, Ugis Sarkans, Steffen Schulze-Kremer, Jason Stewart, Ronald Taylor, Jaak Vilo, Martin Vingron (2001-12). "Minimum information about a microarray experiment (MIAME)—toward standards for microarray data". Nat Genet 29 (4): 365–371. doi:10.1038/ng1201-365. ISSN 1061-4036. PMID 11726920. 
15. ^ Bossuyt, Patrick M.; Johannes B. Reitsma, David E. Bruns, Constantine A. Gatsonis, Paul P. Glasziou, Les M. Irwig, David Moher, Drummond Rennie, Henrica C.W. de Vet, Jeroen G. Lijmer (2003-01-01). "The STARD Statement for Reporting Studies of Diagnostic Accuracy: Explanation and Elaboration". Clin Chem 49 (1): 7–18. doi:10.1373/49.1.7. PMID 12507954. http://www.clinchem.org/cgi/content/abstract/49/1/7. Retrieved 2009-01-13.