Direct fluorescent antibody

Direct FA stained mouse brain impression smear reveals the presence of the bacterium Chlamydia psittaci. 400X.

Direct fluorescent antibody (DFA or dFA) (also known as "Direct immunofluorescence"^[1]) is a laboratory test that uses antibodies tagged with fluorescent dye that can be used to detect the presence of microorganisms. This method offers straightforward detection of antigens using fluorescently labeled antigen-specific antibodies. Because detection of the antigen in a substrate of patient sample (cellular smear, fluid or patient- inoculated culture medium) is the goal, DFA is seldom quantitative.

This is the main test used to detect rabies in animals and requires the examination of brain tissue.

See also

• Lupus band test
• Immunofluorescence

References

1. ^ Pober, Jordan S.; Abbas, Abul K.; Lichtman, Andrew H. (2000). Cellular and molecular immunology. Philadelphia: Saunders. ISBN 0-7216-8233-2. 

External links

• MeSH Direct+Fluorescent+Antibody+Technique

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