A lymphocyte is a type of
white blood cellin the vertebrate immune system. By their appearance under the light microscope, there are two broad categories of lymphocytes, namely the large granular lymphocytes and the small lymphocytes. Functionally distinct subsets of lymphocytes correlate with their appearance. Most, but not all large granular lymphocytes are more commonly known as the natural killer cells (NK cells). The small lymphocytes are the T cells and B cells. Lymphocytes play an important and integral role in the body's defenses.
An average human body contains about 1012 lymphoid cells, and the lymphoid tissue as a whole represents about 2% of the total body weight. It is 20-40% Semester 4 medical lectures at Uppsala University 2008 by Leif Jansson] of all
reference range= (1-5)x109 cells per L of blood.
Types of lymphocytes
The three major types of lymphocyte are
T cells, B cellsand natural killer (NK) cells
Natural killer cells
NK cells are a part of
innate immune systemand play a major role in defending the host from both tumours and virally infected cells. NK cells distinguish infected cells and tumours from normal and uninfected cells by recognizing alterations in levels of a surface molecule called MHC ( major histocompatibility complex) class I. NK cells are activated in response to a family of cytokinescalled interferons. Activated NK cells release cytotoxic(cell-killing) granules which then destroy the altered cells.cite book | last = Janeway | first = Charles | authorlink = Charles Janeway | coauthors = Paul Travers, Mark Walport, and Mark Shlomchik | title = Immunobiology; Fifth Edition | publisher = Garland Science | date= 2001 | location = New York and London| pages = | url = http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=imm.TOC&depth=10| doi = | id = ISBN 0-8153-4101-6.] They were named "natural killer" because of the initial notion that they do not require prior activation in order to kill cells which are missing MHC class I.
T cells and B-cells
T cells and B-cells are the major cellular components of the
adaptive immune response. T cells are involved in cell-mediated immunitywhereas B cells are primarily responsible for humoral immunity(relating to antibodies). The function of T cells and B cells is to recognize specific “non-self” antigens, during a process known as antigen presentation. Once they have identified an invader, the cells generate specific responses that are tailored to maximally eliminate specific pathogens or pathogen infected cells. B cells respond to pathogens by producing large quantities of antibodies which then neutralize foreign objects like bacteriaand viruses. In response to pathogens some T cells, called " helper T cells" produce cytokines that direct the immune response while other T cells, called " cytotoxic T cells", produce toxic granulesthat induce the death of pathogen infected cells. Following activation, B cells and T cells leave a lasting legacy of the antigens they have encountered, in the form of "memory cell"s. Throughout the lifetime of an animal these memory cells will “remember” each specific pathogen encountered, and are able to mount a strong response if the pathogen is detected again.
stem cells differentiate into several kinds of blood cell within the bone marrow.cite book | author = Abbas AK and Lichtman AH | title = Cellular and Molecular Immunology | edition = 5th ed. | publisher = Saunders, Philadelphia |year = 2003 | id = ISBN 0-7216-0008-5] This process is called haematopoiesis. All lymphocytes originate, during this process, from a common lymphoid progenitor before differentiating into their distinct lymphocyte types. The differentiation of lymphocytes follows various pathways in a hierarchical fashion as well as in a more plastic fashion. The formation of lymphocytes is known as lymphopoiesis. B cells migrate to the spleen and mature into B lymphocytes, while T cells migrate to and mature in a distinct organ, called the thymus. Following maturation, the lymphocytes enter the circulation and peripheral lymphoid organs (e.g the spleenand lymph nodes) where they survey for invading pathogens and/or tumour cells.
The lymphocytes involved in adaptive immunity (i.e. B and T cells) differentiate further after exposure to an
antigen; they form effector and memory lymphocytes. Effector lymphocytes function to eliminate the antigen, either by releasing antibodies (in the case of B cells), cytotoxic granules ( cytotoxic T cells) or by signaling to other cells of the immune system ( helper T cells). Memory cells remain in the peripheral tissues and circulation for an extended time ready to respond to the same antigen upon future exposure.
They live weeks to several years, which is very long compared to other leukocytes.
Microscopically, in a
Wright's stained peripheral blood smear, a normal lymphocyte has a large, dark-staining nucleus with little to no basophiliccytoplasm. In normal situations, the coarse, dense nucleus of a lymphocyte is approximately the size of a red blood cell (about 7 micrometres in diameter). Some lymphocytes show a clear perinuclear zone (or halo) around the nucleus or could exhibit a small clear zone to one side of the nucleus. Polyribosomesare a prominent feature in the lymphocytes and can be viewed with an electron microscope. The ribosomesare involved in protein synthesis allowing the generation of large quantities of cytokines and immunoglobulins by these cells.
It is impossible to distinguish between T cells and B cells in a peripheral blood smear. Normally,
flow cytometrytesting is used for specific lymphocyte population counts. This can be used to specifically determine the percentage of lymphocytes that contain a particular combination of specific cell surface proteins, such as immunoglobulins or cluster of differentiation(CD) markers or that produce particular proteins (for example, cytokines using intracellular cytokine staining (ICCS)). In order to study the function of a lymphocyte by virtue of the proteins it generates, other scientific techniques like the ELISPOTor secretion assaytechniques can be used.
circulatory systemthey move from lymph nodeto lymph node. This contrasts with macrophages, which are rather stationary in the nodes.
Lymphocytes and disease
A lymphocyte count is usually part of a peripheral
complete blood cell countand is expressed as percentage of lymphocytes to total white blood cells counted. An increase in lymphocytes is usually a sign of a viral infection (in some rare cases, leukemias are found through an abnormally raised lymphocyte count in an otherwise normal person). A general increase in the number of lymphocytes is known as lymphocytosiswhereas a decrease is lymphocytopenia.
A decrease in lymphocytes occurs when the
human immunodeficiency virus(HIV) hijacks and destroys T cells (specifically, the CD4+ subgroup of T lymphocytes). Without the key defense that these T cells provide, the body becomes susceptible to opportunistic infections that otherwise would not affect healthy people. The extent of HIV progression is typically determined by measuring the percentage of CD4+ T cells in the patient's blood. The effects of other virusesor lymphocyte disorders can also often be estimated by counting the numbers of lymphocytes present in the blood.
Complete blood count
Human leukocyte antigen
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